Química Farmacéutica
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Item Potencial farmacológico in silico de péptidos obtenidos por hidrolisis enzimática de la proteína de quinua(Universidad Santiago de Cali, 2024) Ruiz Beltrán, Daniela; Uzuriaga Perlaza, Laura Mercedes; Liscano, Yamil; Oñate, JosePeptides, organic compounds formed by the union of amino acids, are highly valued in the pharmaceutical field due to their high specificity and low toxicity. However, in vivo research with peptides can be costly and slow. In contrast, in silico research offers significant savings in time and costs, allowing a broader search and quick analysis of possible biological activities. The enzymatic hydrolysis of proteins, a process that breaks down proteins into amino acids to reach peptides, is fundamental in this field. Quinoa, a pseudocereal native to the Andes with a high protein content, has been the subject of research for the development of new drugs. Bioinformatics tools, which predict and analyze the breakdown of chemical bonds in biological molecules, are increasingly used to design and select peptides with biological activities. Using the NCBI biology tool, the sequence of the quinoa protein was obtained and bioactive predictions were made in 5119 peptidic sequences obtained by hydrolysis of the actinidin enzyme. The bioactive peptides found can be the basis for developing new drugs to prevent and treat microbial diseases and cancer, thus improving the quality of life of peopleItem Desarrollo de un método alterno comprendido en la farmacopea estadounidense (USP) para la determinación cualitativa de impurezas de monoestearil maleato y alcohol estearílico presentes en el estearil fumarato sódico por cromatografía de capa fina (CCF)(Universidad Santiago de Cali, 2024) Rojas Daza, Andrés Felipe; Castro Bonilla, Duván Andrés; Flórez López, Edwin; Gutiérrez, HiulderIn the pharmaceutical industry, analytical procedures play a crucial role in ensuring the quality, safety and efficacy of materials/products, where reproducible and reliable results are obtained. In the present study, an alternative methodology to that presented by the United States Pharmacopoeia (USP) was developed in the limit test of stearyl alcohol (AE) and monostearyl maleate (MM) of the raw material Sodium stearyl fumarate (SEF), through the thin layer chromatography technique with the in order to improve the separation and resolution (hue/intensity) of the spots of the compounds of interest because in the current methodology these parameters generate a confusing result for the analyst. Different tests were carried out, experimentally studying the stationary phase, modifying the proportions and solvents of the mobile phase and testing different revealing agents. Finally, an alternate procedure was proposed, where the separation of the spots of interest, their intensity and tonality were improved with RF values for sodium stearyl fumarate, stearyl alcohol and monostearyl maleate of 0,35; 0,65; 0,05; compared to what was obtained in the version of the pharmacopoeial methodology of 0,47; 0,48 and 0,40 respectively, which ensures the reliability of the resultsItem Fabricación de una barra energética a base de Cannabidiol, Sacha Inchi y Quinua(Universidad Santiago de Cali, 2024) Pantoja Saenz, Luis Alberto; Cardona Velásquez, Héctor Fabio; Ciro, Yhors Alexander (Director); Campo Misas, Rafael EnriqueNowadays, consumers of food products are looking for healthier and more nutritious food options to complement their diet and meet their energy needs. For this reason, the energy bar emerges as an alternative for a population with little time available for their required healthy diet, with momentary cravings and for people who perform physical activities, since they are for quick consumption. However, current energy bars have certain disadvantages, such as high content of added sugars, low nutritional content, increased caloric content, the use of artificial ingredients and the absence of dietary fibers. By identifying these problems, it is relevant to look for alternatives of ingredients with high nutritional content to include in energy bars. In this study, an energy bar based on Sacha inchi, and Quinoa was made, adding an added value of Cannabidiol (CBD) for its potential use as an anxiolytic and analgesic. Three energy bar formulations were proposed, and the selected formulation was tested for protein determination using the Kjeldahl method (Soxhlet Extraction), lipid profile by gas chromatography (Flame Ionization Detector), quantification of CBD and THC by liquid chromatography (ultraviolet-visible detector) and microbiological analysis of molds, yeasts, Escherichia Coli and Salmonella. Finally, an affective sensory test was included on 376 people (men and women), from the university community of the Santiago de Cali University based in Cali, aged between 18 and 65 years. A product of 30g per unit was obtained, with a good source of protein (16.2%), excellent source of alpha-linolenic acid (3.3%). In addition, it contains 9.9 mg of CBD (THC-free) and the affective sensory test resulted in a taste acceptance of 86.5%, smell 93.1%, texture 86.7% and color 92.8%, which was quite satisfactoryItem Validación de un método analítico para la cuantificación de impurezas orgánicas de Lisinopril en el producto Lisinopril 10 mg tabletas, por cromatografía líquida de alta resolución (HPLC) y detector UV-PDA(Universidad Santiago de Cali, 2024) Zapata Salcedo, Gina Marcela; Diaz Peláez, Juan Sebastián; Ciro Monsalve, Yhors Alexander (Director); Miller Peluffo, Jhon AlexanderThe purpose of the following work is to obtain a suitable and accessible chromatographic method for the quantification of known and unknown organic impurities in the Lisinopril 10mg Tablet product. For this, it was proposed to carry out the validation of an analytical method, by high performance liquid chromatography (HPLC) and UV-PDA detector. Evaluating 8 performance parameters in accordance with what is indicated by the ICH-Q2 and chapter 1225 of the United States Pharmacopeia – National Formulary (USP-NF): System suitability, specificity, selectivity, stability of the analyte in solution, linearity, accuracy, precision and range. Following the analytical parameters indicated in the USP-NF 2022 Edition Lisinopril monographItem Seguimiento farmacoterapéutico bajo la metodología CTCAE en la evaluación de las reacciones adversas de la Doxorrubicina en pacientes con cáncer de mama polimedicadas(Universidad Santiago de Cali, 2024) Villanueva Gallego, Isabella; Ciro Monsalve, Yhors Alexander (Director); Echeverry Martínez, John JairoThe study deals in depth with adverse drug reactions (ADR) in breast cancer patients treated with Doxorubicin and other drugs simultaneously. It is highlighted that polymedication increases the risk of ADR, presenting interactions in 24% of the drugs administered, especially in the gastrointestinal, hematological and neurological systems. According to Naranjo's algorithm, it was observed that 64% of the ADRs have a possible relationship, 44% a probable relationship and 7% a doubtful relationship. Likewise, using the Common Terminology Criteria for Adverse Events (CTCAE), 85% of the reactions were classified as mild, 9% as moderate and 6% as severe. A remarkably high incidence of 98% was observed in patients with breast cancer, who were also under treatment with Doxorubicin and polymedication, presenting adverse reactions. This finding suggests a significant likelihood that ADRs are related to Doxorubicin administration in this specific population. The study highlights the importance of comprehensive evaluation and monitoring to prevent interactions and ADRs, proposing strategies such as pharmacotherapeutic follow-up, pharmacovigilance and education for medical staff and patients, in order to optimize safety and improve patients' quality of lifeItem Potencial farmacológico In Silico de péptidos obtenidos por hidrolisis enzimática de la proteína de quinua(Universidad Santiago de Cali, 2024) Ruiz Beltrán, Daniela; Uzuriaga Perlaza, Laura Mercedes; Oñate Garzón, José Fernando (Director)Peptides, organic compounds formed by the union of amino acids, are highly valued in the pharmaceutical field due to their high specificity and low toxicity. However, in vivo research with peptides can be costly and slow. In contrast, in silico research offers significant savings in time and costs, allowing a broader search and quick analysis of possible biological activities. The enzymatic hydrolysis of proteins, a process that breaks down proteins into amino acids to reach peptides, is fundamental in this field. Quinoa, a pseudocereal native to the Andes with a high protein content, has been the subject of research for the development of new drugs. Bioinformatics tools, which predict and analyze the breakdown of chemical bonds in biological molecules, are increasingly used to design and select peptides with biological activities. Using the NCBI biology tool, the sequence of the quinoa protein was obtained and bioactive predictions were made in 5119 peptidic sequences obtained by hydrolysis of the actinidin enzyme. The bioactive peptides found can be the basis for developing new drugs to prevent and treat microbial diseases and cancer, thus improving the quality of life of peopleItem Desarrollo de un método alterno comprendido en la farmacopea estadounidense (USP) para la determinación cualitativa de impurezas de monoestearil maleato y alcohol estearílico presentes en el estearil fumarato sódico por cromatografía de capa fina (CCF)(Universidad Santiago de Cali, 2024) Rojas Daza, Andrés Felipe; Castro Bonilla, Duván Andrés; Flórez Flórez, Edwin (Director)In the pharmaceutical industry, analytical procedures play a crucial role in ensuring the quality, safety and efficacy of materials/products, where reproducible and reliable results are obtained. In the present study, an alternative methodology to that presented by the United States Pharmacopoeia (USP) was developed in the limit test of stearyl alcohol (AE) and monostearyl maleate (MM) of the raw material Sodium stearyl fumarate (SEF), through the thin layer chromatography technique with the in order to improve the separation and resolution (hue/intensity) of the spots of the compounds of interest because in the current methodology these parameters generate a confusing result for the analyst. Different tests were carried out, experimentally studying the stationary phase, modifying the proportions and solvents of the mobile phase and testing different revealing agents. Finally, an alternate procedure was proposed, where the separation of the spots of interest, their intensity and tonality were improved with RF values for sodium stearyl fumarate, stearyl alcohol and monostearyl maleate of 0,35; 0,65; 0,05; compared to what was obtained in the version of the pharmacopoeial methodology of 0,47; 0,48 and 0,40 respectively, which ensures the reliability of the results