Microbiología
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Browsing Microbiología by Author "Caicedo Bejarano, Luz Dary"
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Item “Actividad antifúngica de alpinia zerumbet frente a mohos y levaduras de importancia clínica”(Universidad Santiago de Cali, 2020) Cruz Alemán, Lady Stephanie; Benavides Gálvez, Laura Isabel; Caicedo Bejarano, Luz Dary; Castro Narváez, Sandra PatriciaInvasive fungal diseases (EFI) diseases to susceptible hosts, cause high impact morbidity and mortality, associated with the high resistance to antimycotics of the agents etiological associated with this pathology. The antifungal activity of extracts of Alpinia zerumbet, vs. C. albicans, C. parapsilosis, C. krusei, Aspergillus fumigatus, A. terreus and Fusarium oxysporum was determined. The minimum inhibitory concentration (MIC) method and minimum inhibitory fungal concentration (CFI) based on NTC 2455 was used. As control sample, Fluconazole standard is considered and 96% Ethanol as a control. The alcoholic and hydroalcoholic extracts of the leaves and flowers affected good fungistatic and fungicidal activity when compared to the control and standard sample. It is concluded that the leaves compounds and flowers of A. zerumbet are promising for future research looking for compounds with antimicrobial activity for use in agriculture, food, and treatment of systemic mycosis.Item Biota fúngica en fosas nasales de trabajadores de tres edificios de la universidad Santiago de Cali y su relación con síntomas de alergias respiratorias(Universidad Santiago de Cali, 2019) Castillo Tellez, Beatriz Angélica; Caicedo Bejarano, Luz DaryAllergenic fungi enter through the air to nostrils and may be responsible for some respiratory allergies. The objective of the present study was to determine the relationship between allergies diagnosed and undiagnosed according to the results obtained from the adapted survey of ISAAC (International Study of Asthma and Allergies in Childhood) and the fungal load of the nostrils of workers of three buildings of the Santiago de Cali University. The samples taken from the nostrils were made with sterile swabs and the subsequent sowing was done on Sabouraud dextrose agar, sunflower seed agar and Candida CHROMagar. In 100% of the workers (126) who participated in the study, allergenic fungi were isolated. The highest prevalence of allergenic fungi in the workers of the different buildings was obtained by the workers of block 1, finding significant statistical differences, for the genus Fusarium sp, (p=0,0018 Kruskal Wallis) and Penicillum sp, (p=0,0080 Kruskal Wallis), but not for Cladosporium sp, Alternaria sp and Aspergillus sp; likewise, the biggest fungal load was presented by the workers of this building. When analyzing the fungal 6load found in the nasal passages and its relation with the presence of symptoms of respiratory allergies, it was established that there was significant statistical association with the genus Fusarium sp (p=0,0430 Wilcoxon Mann Whitney) and not for Cladosporium sp (p=0,1918, Wilcoxon Mann Whitney) which was the most frequently isolated genus.Item Comparación de diferentes medios de cultivo para el aislamiento de hongos alergénicos a partir de ambientes y fosas nasales(Universidad Santiago de Cali, 2019) Concha Alarcón, Héctor Raúl; Caicedo Bejarano, Luz Dary; Chávez Vivas, MónicaDifferent culture media were evaluated for the collection of environmental samples (Agar Dicloran Rosa de Bengala Cloranfenicol, CHROMagar Candida, Avena Agar and , Sabouraud Dextrosa Agar + 5% NaCl) and nasal fossa samples (Sunflower seed agar, SDA and CHROMagar Candida) of workers from three University buildings Santiago from cali. It was found that there were statistical differences, p>0.05, when CHROMagar Candida and Agar Dicloran Rosa de Bengala Cloranfenicol were compared with the volumetric method. , Sabouraud Dextrosa Agar + 5% NaCl recovered the lowest number of UFC / m3 , p <0.0001 when compared with the other media used with the gravimetric method and in the study of nostrils it was found that SDA was the culture medium where the higher counts P<0.0001. In this study, the Agar Dicloran Rosa de Bengala Cloranfenicol culture medium demonstrated that it is the medium of choice for the isolation of fungi from environmental samples and Sabouraud agar for clinical samplesItem Comparación de dos técnicas para la captación de esporas fúngicas ambientales en un campus universitario(Universidad Santiago de Cali, 2019) Delgado Ñañez, Cristhian; Caicedo Bejarano, Luz DaryThe concordance of two fungal spore uptake techniques in the environments of three buildings of the Santiago de Cali University campus was evaluated. The gravimetric method of spore uptake was compared with the Omeliansky formula and the volumetric method (Air Ideal 3P). The environmental sampling was carried out quarterly for a period of 12 months using Dicloran Rose Bengal Chloramphenicol agar (DRBC). 64497 CFU / m3 were accounted for, of which 34930 CFU / m3 were isolated with the volumetric method and 29567 CFU / m3 with the gravimetric method. No significant statistical differences were observed with the two techniques used (p = 0.0739), with a significance level α = 0.05. The predominant genera obtained with both methods were Cladosporium, Penicillium, Aspergillus and Fusarium, which suggests that although the gravimetric method is not as efficient as the volumetric method, it is reliable for estimating the degree of environmental fungal contamination in a similar way to the volumetric method.Item Estudio de la micota ambiental de tres edificios de la universidad Santiago de Cali, Colombia(Universidad Santiago de Cali, 2019) Lasso Ceballos, Ana María; Caicedo Bejarano, Luz DaryThe quantification of the environmental load in the indoors of the buildings (1, 3 and 4) of the University Santiago De Cali, Colombia and in their corresponding responses to the results to obtain a load value. A volumetric method and Dicloran Bengal chloramphenicol (DRBC) and CHROMagar Cándida culture media were used for sampling. The identification of the fungi was carried out based on macro and microscopic characteristics. Parallel to the sampling the relative humidity and temperature of each space studied was determined. This study was conducted for quarterly periods during twelve months (March 2018 to march 2019). In the study of environments of the three buildings was detected a total of 82393 colony forming units (CFU/m3) of which 60053 CFU/m3correspond to indoor environment. The results obtained showed that it was not possible to establish a correlation between the temperature and the CFU/m3 found inside the three buildings, but for the relative humidity in relation to building 4.The genera Cladosporium, Fusarium, Penicillium and Aspergillus were identified as the most prevalent allergenic fungi in this study. The results in the quantification revealed that the fungal burden exceeds the limits accepted for internal environments (500 CFU/m3) according to the World Health Organization (WHO).Item Sensibilidad cutánea (skin prick test) a hongos alergénicos de los trabajadores de tres edificios de la universidad Santiago de Cali y su relación con los síntomas de alergias respiratorias(Universidad Santiago de Cali, 2019) Calderón Quintero, Cristina; Caicedo Bejarano, Luz DarySe planteó un estudio epidemiológico observacional de corte transversal, se describe una relación a la exposición de hongos en ambientes intramurales y a la presencia de hongos alergénicos en fosas nasales. En el estudio participaron 126 trabajadores de la Universidad Santiago de Cali, quienes firmaron un consentimiento informado y contestaron la encuesta adaptada de ISAAC (The International Study of Asthma and Allergies in Childhood) para determinar la prevalencia de alergias diagnosticadas y no diagnosticadas. 71/126 (56,3%) trabajaban en el bloque 1; 24/126 (19,1%) en el bloque 3 y 31/126 (24,6%) en el bloque 4, La prueba “in vivo” Skin Prick Test (SPT) fue practicada a 75 trabajadores. Se encontró una prevalencia de SPT+ para Fusarium oxysporum de 10/75 (13,3%), Aspergillus fumigatus 5/75 (6,7%), Penicillium notatum 5/75 (6,7%), Alternaría alternata 5/75 (6,7%) y Cladosporium herbarum 1/75 (1,3%). Este estudio permitió establecer una posible asociación entre la presencia de hongos en los ambientes laborales y en las fosas nasales de los trabajadores con la respuesta positiva para la prueba SPT para Fusarium oxysporumItem Susceptibilidad a los antimicóticos de levaduras aisladas de sistemas de aguas de la ciudad de Santiago de Cali, Colombia(Universidad Santiago de Cali, 2019) Osorio Vanegas, Lizeth Stefania; Caicedo Bejarano, Luz DaryWater systems become a high capacity of microorganisms. The objective of this work was to evaluate the susceptibility to the antifungals of yeasts present in the wastewater and natural waters of the city of Cali. Samples were collected from two types of water: potable (Río Melendez, Puerto Mallarino) and residual (Canal Sur, Planta de Tratamiento Cañaveralejo-PTAR). Yeasts were isolated in sabouraud and CHROMagar Candida in the commercial identification with API 20 C AUX (BioMérieux) and in the sequence analysis of the ITS1- 5.8S-ITS2 region. The minimum inhibitory concentration (MIC) of fluconazole and amphotericin was determined by the microdilution method, and the influence of some physicochemical parameters and heavy metals was correlated with the possibility of yeasts and antifungals. With the phenotypic tests, the 15 yeast species are identified and the molecular biology techniques six species, the morphotype of the property that can belong to the new species of the genus Candida. 100% of the strains were sensitive to Amphotericin B, and 44.3% were sensitive, 19.23% were sensitive dose-dependent (DDS) and the remaining 25% were resistant to fluconazole. Finally, a correlation has been found between the minimum inhibitory concentration, some physicochemical parameters and the concentration of heavy metals on the antifungal sensitivity of yeasts associated with aquatic systems in the city of Cali.