Microbiología
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Item Estudios de metagenómica en Colombia para la identificación de bacterias en muestras ambientales: Revisión sistemática(Universidad Santiago de Cali, 2022) Mercado Montilla, Anggie Melissa; Cardona Tobar, Karen MelissaItem Evaluación de la actividad antibacteriana de ampicilina encapsulada dentro de nanoliposomas funcionalizados frente a Staphylococcus aureus(Universidad Santiago de Cali, 2022) Llanten Llanten, Mónica Gineth; Usma Montiel, Alejandra; Oñate Garzón, José Fernando; Salamanca Mejía, ConstainAntibiotic resistance is a major public health problem, as antibiotics are the main tool for treating infectious diseases. This project aims to prepare a carrier for an antibiotic such as ampicillin that has been used in medicine for its broad antimicrobial spectrum against gram-positive bacteria. This carrier will be able to improve its permeability and antimicrobial activity against sensitive and resistant S. aureus. Functionalized liposomes with low molecular weight chitosan were used, which were prepared by the ethanol injection technique and physicochemical characteristics were measured. In addition, antimicrobial activity tests were performed. The results of this work were stable but not acceptable in terms of the elaboration and stability of the nanosystems, but the antibacterial test was not as expected, since the nanosystems did not allow ampicillin to be effective against S. aureus strains by His compositionItem Evaluación de la capacidad antibacteriana y/o sanitizante de extractos de plantas de sambucus spp y salvia spp frente a acinetobacter baumannii y pseudomonas aeruginosa(Universidad Santiago de Cali, 2022) Nasamuez Narváez, Anderson Román; Del Socorro Correa, Adriana María; Hernández Carvajal, Jorge EnriqueThe extracts obtained from the different plants have shown to be an alternative to obtain new compounds with antibacterial or sanitizing capacity, which could be used as a treatment for different diseases caused by bacteria, substituting certain known antibiotics, implemented against some multi-drug resistant microorganisms that affect the population. The purpose of this work is to evaluate the antibacterial potential of natural extracts from Sambucus spp and Salvia spp , plants traditionally used by native communities, against Acinetobacter baumannii and Pseudomonas aeruginosa. For this, the plants were collected in Villagarzón - Putumayo, which were subjected to ultrasonic and Soxhlet extraction processes, with subsequent measurement of antimicrobial activity by the agar diffusion method. No antimicrobial activity was found with the extracts evaluated against any of the bacteria analyzedItem Determinación de la concentración mínima inhibitoria en hongos de suelo expuestos a Clorpirifos(Universidad Santiago de Cali, 2022) López Gil, Angie; González Vargas, Iván Andrés; Corredor Sáenz, Viviana CatalinaIn Colombia one of the pests present in crops, of agronomic importance is the ant of the genus Atta spp, for its control organophosphorus insecticides composed of Chlorpyrifos are used, for this reason in the present study was carried out the isolation and identification of filamentous fungi present in soils associated with anthills. Morphological identification was carried out following the taxonomic keys described by (Samsong & Reeneen-Hoekstra), where the previously isolated fungi were classified according to genus and species. The minimum inhibitory concentration of Chlorpyrifos in these fungi was evaluated. For the determination of this concentration, sowing was carried out in liquid medium (Peptone broth) and solid medium (PDA) with the addition of the commercial insecticide Lorsban®. Four different concentrations were evaluated on the third and sixth day of growth. Forty filamentous fungi were isolated, Penicillium spp being the most prevalent with (13/40) isolates. Tolerance to Chlorpyrifos was presented in different degrees; the genus Trichoderma spp did not show changes at the sixth day in the four dilutions, which indicates that this genus can be used in studies on resistance to ChlorpyrifosItem Identificación de bacterias productoras de betalactamasas de espectro extendido en fertilizantes orgánicos(Universidad Santiago de Cali, 2023) Espinosa Santa, Gabriela; Montero Castrillón, Paola Andrea; Correa Bermúdez, Adriana María (Directora); Falco, Aura Dayana (Directora)Organic fertilizers have played a fundamental role nowadays due to their multiple benefits on soils and on the environment by not producing harmful chemicals for it, without leaving behind certain key factors such as their components to make them more natural, such as the waste produced by livestock. Thus, a search for enzymes encoded by third generation cephalosporin resistance genes was carried out by means of susceptibility tests, synergy tests and PCR from industrial and artisanal fertilizers, which are available for free sale to the public. The results indicated 100% presence of genes related to antibiotic resistance in the 19 isolates analyzed, 68.42% of which were identified as E. coli and 31.58% as K. pneumoniae, with the bla CTX-M gene standing out among them, being present in 84.21% of the isolates. In conclusion, it was confirmed that organic fertilizers can act as a possible source of dissemination of multiresistances.Item Evaluación del cumplimiento de buenas prácticas de manufactura (BPM) en la empresa de lácteos Nasalac de Tacueyó Cauca(Universidad Santiago de Cali, 2023) Ramírez Jaramillo, Margarita; Zúñiga Franco, Edna Rocío; Correa, Adriana del socorro (Directora); Rivera, Sandra Patricia (Directora)Good Manufacturing Practices (GMP) are a set of principles that guarantee the safety of products and reduce risks in the production and marketing chain. The Nasalac company belonging to the indigenous community of Tacueyó in the Department of Cauca, has as its corporate purpose the processing of milk, for the production and marketing of dairy derivatives such as yogurt, however, the quality management system has not been implemented. in accordance with the Current Colombian Regulations and does not comply with the requirements stipulated in the Good Manufacturing Practices program. For this reason, the main objective of this investigation was to evaluate the implementation and compliance of Good Manufacturing Practices in said company through a diagnosis of the current process in which a visual audit of the plant was carried out, guided by resolution 2374 of the 2013, in which it was determined that the company does not comply with the requirements established by the regulations, obtaining a score of 47.77% compliance, showing that the main shortcomings are the poor state of the infrastructure and the lack of implementation of a system quality; Likewise, through the services of the external laboratory, the microbiological quality of the yogurt production process was verified, in which it was possible to demonstrate counts outside the limits established for raw milk, with the presence of mesophilic aerobes, total coliforms, E. coli, and Salmonella spp, the process waters with reports of aerobic Mesophiles and total Coliforms, according to the deficiencies found, an action plan is created so that the company can correct and improve the quality of the final product. According to the above, we can conclude that the implementation of Good Hygienic Practices to companies that manufacture, process and transport food is of utmost importance, since it allows us to guarantee the safety of the food, and allows the company to improve its production chain and expand your marketing.Item Efecto de extractos polares de borojó y cascarilla de cacao sobre la viabilidad de aislados de Enterobacteriaceae presentes en cacao y sobre su producción de biopelículas.(Universidad Santiago de Cali, 2024) Rodríguez Rentería, Luisa Valentina; Fernández Daza, Fabián Felipe (Director); Delgado Ospina, Johannes (Director)In recent years, interest has been aroused by the antimicrobial and biofilm removal properties that some bioactive compounds present in plants may present, including polyphenols. Borojó (Borojoa patinoi) and cocoa shell (Theobroma cacao L.) (byproduct generated after the bean roasting process) are rich in polyphenols and antioxidant compounds. In this work, the effect of polar extracts of Borojó pulp and cocoa shell on the viability of bacterial isolates of Enterobacteriaceae obtained from cocoa fermentation and on their biofilm production was studied. The extracts were obtained by solid-liquid extraction with ethanol: water (1:1) and ultrasound for 80 minutes at 25°C. The Folin-Ciocalteu method was used for the total quantification of phenolic compounds. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the extracts were determined against 15 bacterial isolates belonging to the genera Klebsiella, Enterobacter, and Escherichia and 3 strains of Salmonella, (ATCC) by broth microdilution. The effect of the extracts on biofilm formation was evaluated on a polystyrene surface. The borojó extract presented antimicrobial activity against all the isolates evaluated at concentrations that varied between 66 to 90 mg/mL and a reduction in biofilm formation by more than 88.6%. The cocoa shell extract did not present antimicrobial activity and reduced biofilm formation by 27.7%. Borojó extracts should be considered potential sources of antimicrobial compounds.Item Evaluación de la actividad antimicrobiana contra salmonella serovariedad typhimurium y B. Cereus en extractos de curuba y moléculas bioactivas(Universidad Santiago de Cali, 2020) Guevara Restrepo, Angélica María; Aranaga Arias, Carlos Andrés (Director)The excessive and indiscriminate use of antibiotics in the main human activities has caused in recent years a serious problem of resistance in pathogenic bacterial strains that cause infectious diseases worldwide. As a consequence, some of the main drugs against these diseases are ineffective, generating alerts in the main control entities. This problem has aroused the interest of the scientific community. Some natural compounds extracted from plants have been proposed as viable options for the creation of new drugs with antimicrobial capacity. The objective of this work was to determine the possible in vitro antimicrobial activity of curuba extract and bioactive molecules on the growth of Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028 and Bacillus cereus ATCC 11778, some of the main pathogens that cause gastrointestinal diseases in man. Using the disc diffusion methodology, the antimicrobial susceptibility of 8 previously described bioactive molecules with antiparasitic activity, in concentrations of 10µM and 100 µM, was evaluated. The ethanolic, hexane and methanolic extracts were also evaluated from curuba peel and pulp ( Passiflora tarminiana) at concentrations of 0,5 mg / ml and 1 mg / ml, in addition the agar dilution methodology was used to evaluate the minimum inhibitory concentration (MIC) of those molecules or extracts that showed some type of microbial inhibition. It was observed that both the bioactive molecules and the hexanic extract from the curuba peel and pulp did not show any type of inhibition against the studied bacterial strains, on the contrary, it was evidenced that the methanolic and ethanolic extracts from the curuba pulp they inhibited the growth of Bacillus cereus, being the methanolic extract the one with the highest antimicrobial potential.Item Validación secundaria para la prueba de recuento microbiano y la prueba de microorganismos específicos para nitazoxanida por 500 mg(Universidad Santiago de Cali, 2020) Reyes Moctezuma, Nataly Alexandra; Rivera Sánchez, Sandra Patricia (Directora)The drug Nitazoxanide tablet, coated with 500 mg, is manufactured for certification and export to the main risk areas with protozoal infections, such as: Mexico, western South America, western Africa, South Africa and parts of the Middle East and India. The deep plate microbial count test and the test for specific microorganisms, allow to demonstrate the methodology of the methodology, and can be used as a quality control method, in the plant located in the industrial zone of the city of Cali. The present work was carried out at the pharmaceutical company Sanofi, Cali plant, where the challenge organisms used in each stage of the process were evaluated, respectively the strains ATCC (American Type Culture Collection) Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 65538, Pseudomonas aeruginosa ATCC 9027, Bacillus subtillis ATCC 6633, Aspergillus niger ATCC 16404 and Candida albicans ATCC 10231. The stages of the process were: a) standardization of inoculum, b) productivity and selectivity tests of the culture media, c) development of the technique of Secondary validation for the plate microbial count test and specific microorganism test. During the validation process, the comparison of the results evaluated by three observers is included, demonstrating that the reproducibility complies, since the data shows that the coefficient of variation between each repetition with the different microorganisms analyzed is less than 30%, being very homogeneous; Regarding the accuracy, it is evidenced with the correlation coefficient of agreement between the observer counts, it is very good, it shows results over 80% and a recovery greater than 70% of all the study microorganisms, when compared with the controls. positive, and used in the 1: 1000 dilution in the total count of aerobic bacteria and in the total count of molds and yeasts. The recovery of the validation of the method was demonstrated, for the count of microorganisms and the recovery of the specific microorganism (Escherichia coli).Item Evaluación del efecto inhibitorio de los pigmentos extraídos de Aureobasidium spp frente a Lactobacillus spp. aislados de la fermentación alcohólica(Universidad Santiago de Cali, 2022) Samboni Tenorio, Alejandro; Parrado, Darly Silvana; Aranaga Arias, Carlos AndrésMelanin is one of the most widely distributed pigments in nature and among other functions, it has been attributed to a great antibacterial activity. In the present study, the production of the melanin-type pigment was determined in crude extracts obtained from four environmental isolates of yeast-like fungi of the genus Aureobasdium (LC 112, YAM1, YAM2 and YAM4), as potential inhibitors of bacteria of the genus Lactobacillus contaminating the alcoholic fermentation and the yeast of Saccharomyces cerevisiae alcohol producer. For the production of the pigment, the Aureobasdium isolates were grown in an inducing medium (KH2PO4 0.1%, (NH4)2 SO4 0.05%, MgCl2-6H2O 0.05% and glucose 5%), with constant agitation for 120 hours. To obtain the extract, the cells were harvested, lysed and acetone was used as extraction solvent. Once the extracts were obtained, the Minimum Inhibitory Concentration (MIC) was determined by microdilution assay in broth with resazurin. The results show a higher extraction efficiency in the isolate YAM2 (2.1 g/L) followed by YAM1 (1.66 g/L), YAM4 (1.06 g/L) and LC112 (1.03 g/L).), while the MIC for all tested microorganisms was greater than 2 mg/ml. Since no extract showed antimicrobial activity at the maximum concentration established in this work, it is proposed to quantify the concentration of melanin present in each extract. In this way, a theoretical concentration could be established to carry out microbial inhibition tests that allow establishing the real potential of the use of Aureobasdium extracts in the inhibition of contaminating microorganisms in the alcoholic industryItem Determinación de la actividad antifúngica de extractos vegetales de Piper aduncum y Thevetia peruviana(Universidad Santiago de Cali, 2022) Cossio Banguero, Alejandra; Aranaga Arias, Carlos AndrésThe yeast Candida albicans is an opportunistic pathogen capable of causing serious systemic infections in immunocompromised individuals. Several studies have demonstrated the antimicrobial properties of plant extracts, due to the ability of plants to synthesize a wide variety of bioactive compounds. In this research, the antifungal activity of dichloromethanolic, methanolic and ethyl acetate extracts of Piper aduncum and Thevetia peruviana plants on the growth of C. albicans strains ATCC 14053 and ATCC 10231 was evaluated by agar dilution and broth microdilution to determine the Minimum Inhibitory Concentration (MIC) and establish whether the extracts have fungistatic or fungicidal activity. It was evidenced that the dichloromethane extract of P. aduncum was the only one to present inhibitory activity against the strains at an extract concentration of 1000 µg/mL and presented MICs that were in a range between 256->1024 µg/mL, finally, it was established that the extract presented fungistatic activity as a result of presenting a MIC:MFC (Minimum Fungicidal Concentration) ratio higher than 4. Finally, the presence of medium polarity molecules in the P. aduncum plant with inhibitory activity against C. albicans was indirectly evidencedItem Sulfuro de Cadmio como material bactericida contra Escherichia coli y Staphylococcus aureus(Universidad Santiago de Cali, 2022) Castillo Gaviria, Karol Gabriela; Ávila Torres, Yenny Patricia; Ríos Acevedo, John JairoIn this preliminary research project, the antibacterial activity of the semiconductor Cadmium Sulfide (CdS), obtained from laboratory residues from conventional practices of analytical chemistry and inorganic chemistry, was evaluated. This compound has structural and electronic characteristics that allow its photoactivation under visible radiation at 480 nm, and therefore the generation of reactive oxygen species in the presence of oxygen and aqueous solution. The material was characterized by solid state UV-Vis spectroscopy and scanning electron microscopy (SEM). Due to its ability to interact with radiation, photoactivated CdS, has successfully degraded some organic molecules such as fluoroquinolones in previous work. However, within applications as an oxidant, its use as a disinfectant using the photocatalysis process had not been explored. Reason for which, in this project it was found that this residue can be used against gram positive and gram negative microorganisms, showing that the attack is selective. This mechanism was suggested by using selective scavengers of reactive oxygen species (super-oxo anion radical, hydroxyl radical and photoholes) generated in this environment and under laboratory conditionsItem Validación de la eficacia del programa de higiene y desinfección en tanques de fabricación de productos cosméticos en una empresa de la ciudad de Cali(Universidad Santiago de Cali, 2021) Bianchá Oliveros, Andrés Felipe; Arango Lara, Martha Alicia; Martínez Garay, Carlos AndrésMicrobial quality in cosmetic products that meets the standards for their commercialization, that do not generate sanitary complications to consumers or economic damage to cosmetic product companies, is achieved through rigorous controls during their production, taking certain measures to guarantee their innocuousness. These include the process of Hygiene and Disinfection (H&D) of equipment, an important tool within the microbiological control that guarantees the microbial quality of the product. In the cosmetic industry is necessary to verify this process, mainly in the manufacturing mixers, as these are where the products will be in direct contact with their surfaces until they are packaged. The objective of this work was verify the effectiveness of the H&D process applied to the manufacturing mixers of a cosmetic company in the city of Cali, by analyzing the microbial loads of bacteria and fungi on surfaces. After performing the verification throughout three H&D protocols carried out in the company on different dates, it was determined that in all cases a 100% reduction of the microorganism load was achievedItem Caracterización molecular de bacterias presentes en la planta de tratamiento de lixiviados del antiguo vertedero de navarro, Santiago de Cali, Valle del Cauca(Universidad Santiago de Cali, 2022) Pachón Soto, Ingrid Alejandra; Diazgranados Santos, Maryory Vanessa; Corrales Ducuara, Alba RocíoThe study of environmental ecology is important to understand the functions, applications and use of bacteria with metabolic capacities to degrade organic compounds; therefore it is important to characterize the bacteria that can be found in various contaminated environments, which through molecular identification more accurate information can be obtained on microbial biodiversity. Therefore, the present study identified cultivable bacteria of environmental importance in six lagoons and a pure leachate that are part of a Leachate Treatment Plant (LTP) located in the Old Navarro Sanitary Landfill (AVN) in the city of Santiago de Cali. Forty six morphotypes were isolated and evaluated from the amplification of the 16S gene (16S rRNA) with the universal primers U1/U2. Sequence analysis was performed using bioinformatics programs and 4 main Phylum Firmicutes, Proteobacteria, Bacteroidete and Actinobateria were recorded, of which Firmicutes was the most abundant. In addition, an association analysis was performed and it was shown that two morphotypes were present in all the sampled ponds: they were identified as Bacillus sp., and Bacillus mycoides. This study represents an advance in the recognition of bacterial biodiversity in the six lagoons that are part of the Treatment Plant (PTL) of the Old Navarro Sanitary Landfill (AVN); Obtaining and generating 27 16S rRNA sequences from bacteria isolated from AVN PTL leachate samples. Because there are few studies on the molecular identification of environmental bacteria in leachate samples, this work constitutes a first report of bacterial species present in the six lagoons and pure leachate that are part of the PTL, contributing to the knowledge of biodiversity. Of cultivable bacteria that are present in the study area for future researchItem Evaluación in silico de la interacción de un complejo de coordinación de meropenem con enzimas metalo-betalactamasa tipo VIM(Universidad Santiago de Cali, 2022) Castillo Arce, Sebastián; Ocampo Ibáñez, Iván Darío; Correa Bermúdez, AdrianaGram-negative bacteria are the most common bacterial type that can be found causing serious nosocomial infections. For the treatment of these infections conventionally, beta-lactam antibiotics can be used. However, some bacterial strains have developed mechanisms of resistance to these drugs, including the production of metallo-beta-lactamase enzymes of the VIM type, where they act by forming a covalent union with the carbonyl group of the beta-lactam ring leading to its rupture and subsequently to its hydrolyzation. Recently, coordination complexes have been considered as alternative methods to control these resistant bacteria, seeking that they exceed the enzymatic action and cause the death of the bacteria. However, it is necessary to carry out studies that allow these molecular mechanisms to be verified. In this sense, the present study aimed to evaluate the in-silico interaction of two coordination complexes composed of meropenem and nickel against 7 variants of the metallo-beta-lactamase enzymes type VIM: VIM-1, VIM-2, VIM-4, VIM-6, VIM-8, VIM-19, VIM-24) through predictive modeling of protein structures and molecular docking. One of the complexes evaluated, defined as inverted complex (IC)," showed the most favorable interaction against the VIM-4 enzyme variant with an affinity energy (-8.4 kcal/mol), establishing an interaction with the residues Asn210, Ser207, His116, Asp118, Phe62, Trp87, His179, His240 and Tyr67, in addition to forming types of interactions such as Van Der Waals, Akyll, Pi-akyll, Sulfur-X, Pi-sulfur and Pi-sigma, metal acceptor ions and hydrogen bondsItem Validación de la prueba de retención bacteriana para el producto inyectable Medroxiprogesterona acetato 150mg bajo los criterios del informe técnico N.26 de la PDA y la norma ASTM F838-15A.(Universidad Santiago de Cali, 2022) Bastidas Basante, Jhosed Yamid; González Vargas, Iván Andrés; Acevedo Estupiñán, José NicolasThe validation of the bacterial retention test is a test required by Colombian resolution 1160 of 2016, in order to demonstrate that the filters used in the sterilization of products are capable of retaining microbial contamination. For this reason, the described report 26 of the association of parenteral drugs and the ASTM F838-15A standard were used as a reference to validate the filters used in the sterilization of the product medroxyprogesterone acetate 150mg, in this way, the design of the bacterial retention system was carried out, followed by the standardization of the inoculum, continuing with the evaluation of the viability of the microorganism with respect to the product by direct inoculation and subsequently proceeded with the bacterial retention test. The microorganism proved to be viable in only one of the two solutions of the product and the evaluation of the retention resulted in obtaining a sterile effluent. Finally, it was shown that the filters have the ability to retain a concentration > to 1x107 CFU/cm2 of effective filtration areaItem Evaluación de la interacción de dos péptidos catiónicos sobre membranas de Klebsiella pneumoniae sensible mediante dinámica molecular(Universidad Santiago de Cali, 2022) Cortés Barbosa, Valeria Andrea; Rivera Sánchez, Sandra Patricia; Oñate Garzón, José FernandoThe study of the sensitivity of microorganisms to antimicrobials is one of the most important functions of clinical microbiology laboratories. One of the main factors why bacterial strains (including Klebsiella pneumoniae) have lost sensitivity is due to the continuous use and misuse of antibiotics. Bacteriological research over the last few years shows the rapid progression of bacterial resistance to antibiotics. in fact, each time a new antibiotic has been used, bacteria have adapted to it more rapidly. The World Health Organization (WHO) emphasizes the importance and relevance of this serious global health problem and the need to develop new antibiotics that exert their activity on targets other than conventional antibiotics, such as antimicrobial peptides (AMPs). In this study the objective was to evaluate the interaction of two peptides one ΔM2 derived from Cecropin D with 39 amino acids and the other buforin I in model membranes mimicking wild-type Klebsiella pneumoniae bacteria by in silico molecular dynamics using GROMACS software. The in silico study was performed to predict specific interaction between the amino acids of ΔM2 and buforin I and the lipids of the model membranes. The analysis revealed that the long peptide ΔM2 derived from Cecropin D with 39 amino acids and buforin I, presented a greater interaction with the phospholipid 1-Palmitoyl-2-oleoyl-sn-glycerol-3-phosphatidylethanolamine (POPE) forming hydrogen bridge type interactions, an important quality for the stability of the peptides and also allowing contributions to strengthen further studies which help in the development of new drugsItem Comparación de mecanismos de resistencia y virulencia de aislados ambientales de Pseudomonas putida como factores asociados a patogénesis en aislados clínicos(Universidad Santiago de Cali, 2022) Jiménez Mena, María Alejandra; Del socorro Correa, Adriana María; Falco Restrepo, AuraPseudomonas putida is a microorganism that inhabits terrestrial and aquatic environments, but has now been reported as an emerging pathogen in the hospital environment. In recent years there has been a growing increase in infections associated with health care (IAAS) by this species, especially in immunosuppressed patients, which has gained great relevance because it can be found widely distributed in inanimate hospital surfaces and in humid environments due to its tolerance to harsh environmental conditions. The aim of this work is to identify virulence factors and antimicrobial resistance mechanisms in environmental P. putida isolates that may be associated with the pathogenesis of clinical isolates.Therefore, through bioinformatics tools, a comparative analysis of resistance genes and virulence factors in complete genomes of clinical and environmental isolates of Pseudomonas putida reported in NCBI was performed (National Center for Biotechnology Information). The identification of resistance genes and virulence factors took into account the annotations made by the authors and the PATRIC server 3.6.12. The MAUVE programme was used to carry out the comparative genomic analysis. Eleven isolates were found, of which 18.2% were clinical, and 81% were environmental. In terms of resistance genes, a total of 44 genes were found, of which 44 (32%) belong to expulsion pumps, porins (9%), quinolones (11%), aminoglycosides (7%) among others. On the other hand, 30 virulence factors were found associated with motility (43%), biofilm formation (23%), metabolic factor associated with pyverdine synthesis (16%), among others. Comparative genomic analyses indicated 177 and 77 homologous regions among clinical and environmental isolates, which may favor infectious processes in humansItem Comparación de mecanismos de resistencia y virulencia de burkholderia cepacia complex entre aislados clínicos y ambientales(Universidad Santiago de Cali, 2022) Ortiz Vallecilla, César Giovanni; Correa Bermúdez, Adriana María Del Socorro; Ibáñez Ocampo, Iván DaríoBurkholderia cepacia complex (Bcc) is one of the most common microorganisms found in the environment; it has been widely used in agriculture as a biological controller and in bioremediation processes. These activities can lead to contamination of water and food and in turn cause infectious processes in humans. Infections by this microorganism are complex because this bacterium has virulence and resistance mechanisms that allow it to adapt to different environments including clinical. The objective of the study is to identify the virulence and resistance factors of environmental isolates of Burkholderia cepacia complex that may favor infectious processes in humans. This was carried out with the help of bioinformatics tools with which analyses were made relevant to the comparison of resistance genes and virulence factors in genomes of clinical and environmental strains of Bcc, which we found using the NCBI database (National, Center for Biotechnology Information); in this platform we also made a first manual review of those genes of interest for our project. For a second review of the genomes in the search for resistance genes and virulence factors we use the tool PATRIC 3.6.12. Finally, a comparative genomic analysis was performed using the MAUVE tool. Once this was done, it was possible to find 13 complete genomes, of which 12 belonged to the environment and 1 to the clinical environment. The results showed that the main problem of Bcc is that it is intrinsically resistant to multiple antibiotics where the main resistance mechanisms include the presence of ejection pumps, the inactivation of antibiotics by means of enzymes such as β-lactamases and the ability to form biofilms. On the other hand, ejection pumps, porins and B-lactamases are present, other mechanisms associated with their intrinsic resistance were also identified, which are very important at the clinical level, such as resistance to quinolones, aminoglycosides, phosphomycin and polymyxin. It should be mentioned that the genes found in the clinical strain belong to the group IMMUNE MODULATION, are considered antiphagocytosis and have the particularity of possessing a capsule. The comparative genetic analysis showed that there is a great homology between the cynical and environmental strains, giving rise to strains of Bcc clinics that can prevent from the environment giving the connotation of emerging microorganism and being very careful in hospital infectionsItem Gametocitos de plasmodium vivax como marcador de transmisión en tierralta, departamento de Córdoba-Colombia(Universidad Santiago de Cali, 2022) Valencia Salazar,Leidy Julieth; Rivas Asprilla, Yumary; Vera Lizcano, Omaira; Yasnot, Maria FernandaMalaria remains a public health problem in Colombia. In the country, endemic transmission is maintained in the areas that have historically presented the burden of this disease and little is known about the individual social determinants related to the disease. The study aimed to analyze factors associated with the transmission of the Plasmodium vivax parasite in malaria-endemic areas. 50 samples collected in 2016 in the Tierralta department of Cordoba were diagnosed with coarse drop. In gametocyte count it was associated with individual and parasitic socio economics determinants through wilcoxon tests, kruskal-wallis and Spearman correlation, at a significance level of 5%. Association of gametocytes with ethnicity, parasitemia and asexual stages was found. Knowledge about the biology of gametocytes could give guidelines for implementing control measures that reduce malaria figures in different endemic regions