Facultad de Ciencias Básicas
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Item Activación foto- catalítica de nano partículas de MnO2 obtenidas a partir de ablación laser, aplicada a superficies auto-limpiantes(Universidad Santiago de Cali, 2021) Villegas Díaz, Claudia Lorena; Ávila Torres, Yenny Patricia; Ríos Acevedo, John JairoThe nano-particles of metallic materials have many applications, a field of special interest is the hospital, since there are many infections associated with health care (HAI) these infections must be related to hospitalization or procedures performed in the hospital. Mainly originated by Staphylococcus aureus and Escherichia coli, for this reason materials are required that are capable of eliminating these bacteria attached to it, and thus prevent the high number of infections (self-cleaning surfaces). A new material with these characteristics is manganese oxide (MnO2), used in the manufacture of steel, with which hospital equipment is manufactured. In this study, different factors affecting bactericidal photocatalysis were tested, showing that the attack is discriminated for gram positive and gram negative bacteria, the action mechanism is governed via hydroxyl radicals and to a lesser degree via superficial voids. Finally, a material with little recombination is obtained, which represents a success in the synthesis of metal oxide.Item Actividad antifúngica de alpinia zerumbet frente a mohos y levaduras de importancia clínica(Universidad Santiago de Cali, 2020) Cruz Alemán, Lady Stephanie; Benavides Gálvez, Laura Isabel; Caicedo Bejarano, Luz Dary; Castro Narváez, Sandra PatriciaInvasive fungal diseases (EFI) diseases to susceptible hosts, cause high impact morbidity and mortality, associated with the high resistance to antimycotics of the agents etiological associated with this pathology. The antifungal activity of extracts of Alpinia zerumbet, vs. C. albicans, C. parapsilosis, C. krusei, Aspergillus fumigatus, A. terreus and Fusarium oxysporum was determined. The minimum inhibitory concentration (MIC) method and minimum inhibitory fungal concentration (CFI) based on NTC 2455 was used. As control sample, Fluconazole standard is considered and 96% Ethanol as a control. The alcoholic and hydroalcoholic extracts of the leaves and flowers affected good fungistatic and fungicidal activity when compared to the control and standard sample. It is concluded that the leaves compounds and flowers of A. zerumbet are promising for future research looking for compounds with antimicrobial activity for use in agriculture, food, and treatment of systemic mycosis.Item Actividad antimicrobiana de Fitocannabinoides como principios activos en plantas medicinales: una revisión sistemática(Universidad Santiago de Cali, 2024) Rivera Vargas, María Yuliel; Suarez Osorio, Liseth; Falco Restrepo, Aura DayanaPhytochemical compounds have long been an alternative to traditional medicine, which is why the industrial sector is dedicated to the manufacture of products such as drugs, cosmetics and veterinary products, and have considered innovating their lines from raw materials derived from the extraction of active compounds from plants. In this way, plant material with various attributes or substances that are allowed and regulated by government entities for use and marketing has been sought. Due to the above, it was proposed to carry out this systematic review that aims to identify medicinal plants that contain phytocannabinoids and that have antimicrobial activity. The consulted databases corresponding to 83, of which documents dating from the years 2010 to 2022 were found, however, 24 were discarded, since they were related to clinical aspects of veterinary use and others due to the years of publication, the which are outside the range of the minimum 10 years to be taken into account for the development of the systemic reviewItem Actividad enzimática de levaduras recolectadas de diferentes ambientes del Valle del Cauca(Universidad Santiago de Cali, 2020) Olaya Hurtado, Dora Stefany; Ossa Cabra, Liseth Tatiana; Martínez Garay, Carlos Andrés; Ramírez Castrillón, MauricioAgro-industrial waste affects the environment, which makes it a current problem. A sustainable strategy is the use of microorganisms, such as yeast, to metabolize and to degrade these compounds, which will allow the development of bioremediation strategies. In this research, we tested yeasts from the mycoteca of Universidad Santiago de Cali, collected from different environments of Valle del Cauca, to determine their enzymatic activity. For this, they were grown in media with different substrates, to evaluate their ability to degrade them through the determination of an enzymatic coefficient (PZ), which takes into account the degradation halos formed. A total of 95 yeast strains were evaluated, of which 37 strains presented some enzymatic activity. Several strains showed potential in this field; the LN6A, P4, LC071A, YAM1, and YAM3 strains had the highest enzymatic activity; while some had the ability to degrade or metabolize two different substrates (LN4A, MRC33, LC112, P4, LN7C) or up to three types of substrate (MRC39). In addition to this, the LN4A strain, from the Navarro lagoon and identified as Candida tropicalis, presented a higher enzymatic activity in two types of substrate.Item Aislamiento de bacteriófagos provenientes de una muestra de origen ambiental con capacidad de infectar salmonella spp.(Universidad Santiago de Cali, 2020) Álvarez Pérez, Juan Martín; Falco Restrepo, Aura DayanaSalmonellosis is a Foodborne Disease (FDB) that occurs due to contamination by bacteria of the Salmonella genus in edibles such as chicken and pork, as well as in products from poultry farms. Because the indiscriminate use of antibiotics in chickens has contributed to the selection of resistant bacteria, it is necessary to implement natural alternatives that allow the control of bacterial populations in poultry farms, bacteriophages being a possible option due to their high specificity to infect bacteria. Using the double layer method, bacteriophages were found in an environmental sample of chicken manure from a poultry farm located in the municipality of Palmira, Valle del Cauca, capable of infecting a strain of Salmonella enterica subsp. enterica ATCC 14028.Item Aislamiento, identificación y caracterización de bacterias ácido acéticas (BAA) para la producción de vinagre a partir de vino tinto(Universidad Santiago de Cali, 2021) Portilla Sinisterra, Andrés Fernando; Vera Lizcano, OmairaStudies of the microorganisms associated with the fermentation processes, their identification and the standardization of their biotechnological use, are of great interest for the economic and technological growth of a country. In this study, a winemaking protocol was obtained to later produce vinegar for human consumption through acetic fermentation, which is carried out mainly by acetic bacteria under aerobic conditions. 10 batches of wine were produced from Isabella grapes, obtained in the municipality of Ginebra-Valle del Cauca, an alcohol percentage between 9,8 – 11,2% was reached, to later obtain acetic acid. The Orleans method was used to make vinegar, which consists of stationary oxidation of ethanol in acetic acid, under the conditions required for the growth of acetic bacteria, from which a concentration of acetic acid between 47,4 - 60 g / L. Subsequently, the bacteria of the Acetobacteraceae family were isolated by means of cutives and identified by means of biochemical techniques. Later, with the Vitek 2 team, the Acetobacter acetti species was identified as the acetic bacteria found in vinegar, it was cryopreserved. This microorganism can be used to produce balsamic vinegar, which is of great commercial interest.Item Análisis estructural y supramolecular del compuesto (e)-3',6'-dihidroxi-2-((pyridina-2-yl metileno) amino) spiro[isoidolina-1,9'-xanteno]-3-ona(Universidad Santiago de Cali, 2019) Cardona Restrepo, Camila; Fernando D¨vries, RichardThe crystalline structure of the compound (E) -3 ',6'-dihydroxy-2-((pyridine-2-and methylene) amino) spiro [iso-idoline-1,9'-xanthene] -3-one was solved and refined, using single crystal X-ray diffraction data. It was found that the compound crystallized in the triclinic P-1 space group. its unit cell present two molecules per asymmetric unit, which correspond to molecular conformers. Moreover, an analysis to determine the intermolecular interactions from the crystalline system was performed. It was observed that the crystal packing is formed mainly by hydrogen interactions type O-H••• N, and C-H•••O. Finally, the supramolecular interactions of the dimer were quantified using fingerprint plots, obtaining values of 41.4%, 12.9%, 11.1% and 10.4% for interactions type H•••H, C•••H, O•••H, H•••C respectively. The aforementioned diagrams were compared with the obtained for structurally similar molecules; their forms confirmed the differences at the supramolecular level of the compounds analyzed. With this project, it was possible to determine the structural and supramolecular characteristics of the compound. In addition, were acquired the basic concepts related to crystallography and supramolecular chemistry, both at a theoretical and experimental level.Item Análisis metataxonómico de suelos de cultivos de yuca (Manihot esculenta Crantz) con síntomas de cuero de sapo(Universidad Santiago de Cali, 2024) Díaz Marín, Valentina; Ocampo Ibáñez, Iván Darío“Frogskin disease” mainly affects the roots of cassava and generates symptoms that deteriorate large quantities of crops. The International Center for Tropical Agriculture (CIAT) in Colombia has been studying this disease in cassava crops for approximately 50 years and has proposed some associated pathogens, however, the causal agent is still unknown. Therefore, in the present study, a meta-taxonomic analysis of the bacteria and fungi that are present in soils of cassava crops with symptoms of the “Frogskin disease” disease was carried out to find the possible causal agents. For this, a metataxonomic analysis was carried out, using Illumina Miseq sequencing, to obtain fungal ITS and bacterial 16S sequences. Bacteria belonging to the phyla Proteobacteria and Firmicutes were obtained; mainly the genera Pantoea, Enterobacter, Bacillus, and Rhizobium. The phyla Ascomycota and Basidiomycota were the most abundant in the fungal ITS communities; represented by the genera Fusarium, Aspergillus, and Alternaria. The analysis of alpha and beta diversity using the Miseq sequencing platform revealed through a one-way ANOVA and a PERMANOVA that there were significant differences in composition at the genus level in soils with disease symptoms and control soils. This study provided useful information on the microbiome present in the rhizosphere and roots of resistant plants infected with “Frogskin disease” symptomsItem Aplicación de la ecuación de Schrödinger en el estudio del movimiento de moléculas diatómicas(Universidad Santiago de Cali, 2019) Bueno Barbosa, Douglas Andrés; Parada Valencia, AlexanderUnderstanding the physiochemical phenomenon at the subatomic level is very important in order to know the behaviour of the matter these energy scales. It is necessary to use the Schrödinger equation in the order to describe chemical and physically the molecules. Throughout of the development of this work, we addressed the study of the Schrödinger equation and applications in the movement of diatomic molecules. The interaction potential is described by this equation, as well as the translational, rotational and vibrational energies of each molecule. Likewise, the Hamiltonian is studied in a general way, and particularly for the hydrogen molecule. We learned the main knowledge relating with Quantum Chemical and after that we focus in the study of some diatomic molecules no studied in the literature until now. Then, a phenomenological study was carried out for the BrCl molecule.Item Aplicación de la espectroscopia de infrarrojo cercano (NIRS) en productos cárnicos(Universidad Santiago de Cali, 2024) Rosero Martínez, José Miguel; Castro Narváez, Sandra Patricia (Directora)An approximation is made to near infrared spectroscopy (NIRS) as a tool for evaluating the quality and authenticity of meat products. Given the growing demand for organic meat products and the need to guarantee their integrity, NIRS stands out as an effective alternative to traditional methods. The methodology employed included literature review, data analysis and method comparison, highlighting the importance of developing more robust calibration and standardization techniques. Quality parameters that NIRS can assess, such as moisture, fat, protein, color and pH, were identified, demonstrating its ability to provide rapid and non-destructive results. Comparison between NIRS and traditional techniques such as proximate analysis revealed that NIRS offers significant advantages in terms of speed and reduced need for sample preparation. However, limitations have been identified, including the need for specific calibrations for each type of meat and the dependence on the quality of the equipment used. Recommendations include advancing the development of calibration techniques and exploring the integration of NIRS technology in real-time computing environments.Item Aplicación del diseño de mezclas en la formulación de sidra de manzana(2020) RAMÍREZ NARANJO; NATHALIA, SINISTERRA AMÚ; DIANA; Ramirez Navas; Juan SebastianCider is a drink with very marked characteristics with respect to other spirits; It has abundant carbon dioxide, but produces less foam than a regular beer, acquires shades of yellow in transparent and bright appearance. (Drilleau, 2005). Cider. 2. The objective of this research was to evaluate the ratio of cider mixtures from three varieties of apple (Malus Domestica): Gala, Golden and Green.Item “Aplicación del diseño de mezclas en la formulación de sidra de manzana (malus domestica)”(Universidad Santiago de Cali, 2020) Ramírez Naranjo, Nathalia; Sinisterra Amú, Diana; Ramírez Navas, Juan SebastiánCider is a drink with very marked characteristics with respect to other spirits; It has abundant carbon dioxide, but produces less foam than a regular beer, acquires shades of yellow in transparent and bright appearance. (Drilleau, 2005). Cider. 2. The objective of this research was to evaluate the ratio of cider mixtures from three varieties of apple (Malus Domestica): Gala, Golden and Green. This mixing design consisted of applying a planning and analysis methodology which could ensure that we obtain the knowledge and solutions. The adoption of this methodology aims to assess the influence of each component of the formulation on the selected response varieties. For this purpose, different physicochemical tests were performed on each mixture obtained: (pH, Density, Volatile Acidity, alcoholic Strength and Sugar Percentage) and from which the expected results were obtained with an average of pH between 3.6 - 3.8; Density between 0.99-1.02 g/mL; Volatile Acidity between 0.04 -0.09 g/L; Alcohol content between 0.73 - 2.4 %V; Microbiological: (Count of aerobic bacteria and mesophylls, mold count, yeast count and total coliforms) whose result of each test was <10 UCF/mL and Sensory Analysis in which preference was found for the sample called B, corresponding to the mixture 0,1/2,1/2 being this the mixture between green apple and gallium apple. The results obtained in each test were expected indicating that the drink does meet the requirements established by the Colombian technical standard NTC 222 and NTC 708, concerning alcoholic beverages and their general definitions, alcoholic beverages (Fruit wines) respectivelyItem Aproximación metagenómica para la identificación de bacterias endófitas de la raíz de yuca (manihot esculenta crantz) bajo condiciones de estrés abiótico(Universidad Santiago de Cali, 2020) Fernández Gaviria, Alexandra; Herrera Arbeláez, Valentina; Corrales Ducuara, Alba RocíoCassava (M. esculenta) is the fourth most important crop in the world. It is considered a basic food in the diet of millions of people in tropical and sub-tropical areas. However, its productivity has been affected by adverse environmental conditions such as: low and high temperatures. In this regard, it is important to study new tolerant mechanisms to different types of abiotic stress. Knowing the endophyte microbial diversity of the root may influence positively or negatively in its development, productivity and crop heath under abiotic stress conditions. Cassava plants M. esculenta of the variety MCOL1734 were subjected to stress by hydric deficit for a 20-day period. In the metagenomic analysis of endophyte bacteria there were four Phylum; Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria, which are cataloged as endophytes in root of plants. Species like; Clostridium sp. Acinetobacter lwoffii and Acinetobacter sp. which are PGPR bacteria, are candidates to implement in improvement programs to increase tolerance to water deficit in cassava cultivation (M. esculenta).Item Bacterias potencialmente degradadoras de hidrocarburos aisladas en Santiago de Cali, Valle del Cauca(Universidad Santiago de Cali, 2020) Machacado Salas, Maryuris; Rache Arce, Diana Carolina; Rosero García, Doris AmandaOil hydrocarbon pollution is one of the main problems around the world since it generates negative ecological, economic, and social impacts. Colombia has been affected by hydrocarbon spills that contaminate different environments such as water and soil. Bacteria have been reported with the ability to degrade hydrocarbons, becoming an excellent strategy for designing studies that contribute solutions to this problem. In the present study, a methodology was standardized to isolate hydrocarbon degrading bacteria in six biological oxidation lagoons and a pure leachate from a treatment plant located in the Old Navarro Landfill at Santiago de Cali city. Three tests were conducted: leachate contamination, disk test and tube test, and 17 bacterial morphotypes with degrading potential of gasoline, diesel and ACPM were found. The bacteria identified were Serratia marcescens (morphotype 97), Bacillus sp. (morphotypes 90, 117 and 263), Bacillus cereus (morphotype 120) and Exiguobacterium sp. (morphotypes 123 and 137). This work carried out to standardize a methodology to isolate bacterial morphotypes in the Old Navarro Landfill and allowed future studies to study the degradation mechanisms in bacteria, and solutions that contribute to the reduction of hydrocarbon contamination.Item Bacterias presentes en suelos contaminados con mercurio y su uso potencial en tecnologías de biorremediación: Una revisión sistemática(Universidad Santiago de Cali, 2022) Valencia Victoria, Isabela; Ramírez Mosquera, Katheryn Nohelia; Falco Restrepo, AuraItem Biota fúngica en fosas nasales de trabajadores de tres edificios de la universidad Santiago de Cali y su relación con síntomas de alergias respiratorias(Universidad Santiago de Cali, 2019) Castillo Tellez, Beatriz Angélica; Caicedo Bejarano, Luz DaryAllergenic fungi enter through the air to nostrils and may be responsible for some respiratory allergies. The objective of the present study was to determine the relationship between allergies diagnosed and undiagnosed according to the results obtained from the adapted survey of ISAAC (International Study of Asthma and Allergies in Childhood) and the fungal load of the nostrils of workers of three buildings of the Santiago de Cali University. The samples taken from the nostrils were made with sterile swabs and the subsequent sowing was done on Sabouraud dextrose agar, sunflower seed agar and Candida CHROMagar. In 100% of the workers (126) who participated in the study, allergenic fungi were isolated. The highest prevalence of allergenic fungi in the workers of the different buildings was obtained by the workers of block 1, finding significant statistical differences, for the genus Fusarium sp, (p=0,0018 Kruskal Wallis) and Penicillum sp, (p=0,0080 Kruskal Wallis), but not for Cladosporium sp, Alternaria sp and Aspergillus sp; likewise, the biggest fungal load was presented by the workers of this building. When analyzing the fungal 6load found in the nasal passages and its relation with the presence of symptoms of respiratory allergies, it was established that there was significant statistical association with the genus Fusarium sp (p=0,0430 Wilcoxon Mann Whitney) and not for Cladosporium sp (p=0,1918, Wilcoxon Mann Whitney) which was the most frequently isolated genus.Item Calificación de un dispositivo de inmersión para así realizar recubrimientos de TiO2/Hidroxiapatita sobre superficies metálicas(Universidad Santiago de Cali, 2019) Martínez Bellaiza, Daniela; Morales Morales, Jimmy Alexander; Castillo Ayala, Jorge EnriqueEl método de inmersión o “Dip-Coating” es utilizado para la obtención de películas delgadas sobre diferentes superficies y con un amplio rango de aplicación; este consiste en la inmersión y posterior extracción de un sustrato dentro de una solución precursora para la formación de la película. En este trabajo se realizó la calificación de un dispositivo de inmersión de bajo costo elaborado en la Universidad Santiago de Cali y se ejecutaron recubrimientos sobre sustratos de acero inoxidable con TiO2/Hidroxiapatita por la técnica de química húmeda. Para la calificación se evaluaron parámetros de velocidad y tiempo de inmersión, abordando la calificación de instalación, desempeño y diseño (IQ, PQ y QD respectivamente); la síntesis de Hidroxiapatita se realizó por precipitación controlada y se analizaron las muestras secas por IR/ATR y TGA-DSC obteniendo unos picos característicos del compuesto y un buen comportamiento térmico, además se analizaron los revestimientos realizados utilizando técnicas espectroscópicas obteniendo los depósitos esperados en los sustratos y obteniendo una relación de Ca/P de 1,57.Item Caracterización de bacterias asociadas a la rizosfera de plantas de yuca (manihot esculenta crantz) sometidas a estrés por déficit hídrico(Universidad Santiago de Cali, 2019) Galindo Espinal, Diana Marcela; Zapata Gualtero, TatianaCassava (Manihot esculenta Crantz) is one of the most important energy sources in the tropical and subtropical regions of the world. During the last years, its production has resulted in extreme environmental conditions. Plant growth promoting rhizobacteria (PGPR) are a type of bacteria that colonize the roots and a symbolic relationship with the plant, favoring their growth and resistance even in conditions of abiotic stress. In the present study, 124 bacterial components of cassava plants M. esculenta are characterized, of which 26 rhizobacteria are identified in plants over a period of 20 days. The analyzes of the abundance of the most predominant genera are: Bacillus, Achromobacter, Pseudomonas, Sphingomonas and Streptomyces. These results are important for bacterial communities such as cassava cultivation and its relationship with the response of plants to abiotic stress conditions.Item Caracterización de incidencias de reacciones adversas medicamentosas asociadas a anticoagulantes y diseño de guía de uso clínico para pacientes anticoagulados(Universidad Santiago de Cali, 2024) Vélez Hernández, Natalia; Rios Acevedo, Jhon Jairo; Echeverry Martínez, John JairoPathologies associated with poor blood coagulation such as DVT (deep vein thrombosis), stroke (cerebrovascular accident), venous thromboembolism, pulmonary embolism, among others; These require a drug that helps the clot that is produced inside a vein or artery not to occlude it, at this point is when anticoagulants play a very important role, being one of the most widely used groups of drugs in Colombia, for this reason a retrospective study was conducted by reviewing 150 clinical histories of anticoagulated patients in the health provider institution Hospital Universitario del Valle, in the period from 2018 to 2022, in order to identify what is the incidence of a patient to present or not an ADR (adverse drug reaction) or AE (adverse event). The age group between 40-90 years was identified, where the prevalence of presenting an undesired reaction during treatment varied according to the anticoagulant used: for Enoxaparin it was 74%, for Dalteparin it was 71%, for Apixaban it was 40% and for Heparin it was 89%. On the other hand, it was possible to identify that the most frequent adverse event was nausea with approximately 40%. Following this, the Chi-square test was performed, where correlation was observed between different variables such as the adverse event presented, the concentration of the anticoagulant and the duration of treatment; by means of these data, it was observed which of the hypotheses was fulfilled for each type of analysis, finding that there is no relationship of influence in terms of age vs. anticoagulant administered and age vs. frequency of presenting an undesired event, while there was a relationship between the other cases already mentioned.Item Caracterización de poblaciones bacterianas como potenciales optimizadores en el proceso de compostaje de la empresa Incauca S.A.S.(Universidad Santiago de Cali, 2021) Díaz Caicedo, Lizeth Natalia; Corrales Ducuara, Alba Rocío; Mercado Álvarez, Ana MaríaComposting is a totally aerobic bioremediation process, in which microorganisms that rapidly and effectively degrade organic matter act. One of the great challenges of the sugar sector is to optimize the degradation times of the organic waste produced by the processing of sugar cane and fuel alcohol. Currently, INCAUCA S.A.S has implemented a sustainable management for waste management, which requires efficient methodologies for the processing and degradation of sugar cane products. In this study, efficient bacteria were characterized in two composting plants of the company INCAUCA S.A.S, as a sustainable strategy to optimize the process of degradation of organic matter. Genera such as Pseudomonas, Bacillus and Aeromonas were identified with an identity greater than 97% indicating having an important role in the composting process, 13 isolates presented halos of cellulose degradation.