Facultad de Ciencias Básicas

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Diseño, implementación y mejoramiento de un método para la disminución del desperdicio generado durante el empaque y embalaje de harina precocida de maíz en la empresa alimentos del Cauca S.A.
(Universidad Santiago de Cali, 2012) Valderrama González, Luis Alfonso; Garzón Garcia, Guillermo
Using statistical process control as a tool for improvement, improved packaging process, integrating teams and encouraging the participation of the same. The application of this method of analysis of a representative sample packs precooked cornmeal able to reduce wastage of the same. This involved the development of technical and administrative skills to solve problems. It also encouraged the participation of the company's operating personnel. Developing this project has managed to launch the appropriate methodology to the particular case, facilitating the organization of control instruments for proper resource management plan.
Caracterización fisicoquímica funcional del Almidón extraído de la papa china (Colocasia Esculenta) cultivada en el pacífico colombiano
(Universidad Santiago de Cali, 2019) Hurtado Herrera, Janer Guillermo; Castillo Ayala, Jorge Enrique
The evaluation of the physicochemical and functional properties of the starch extracted from the Chinese potato tubers (Colocasia esculenta) from the municipality of Timbiquí and López de micay – Cauca was carried out. The granules had particle size around (3.5-7.8 µm in diameter for sample 1 and 3.5-7.0 µm in diameter for sample 2); a high content of % amylose (67.6 and 70.7 respectively for M1 and M2) was found compared to other botanical sources, through the colorimetric method recommended by ISO 6647. The gelatinization temperatures were (78, 6 and 81.9 ° C, respectively) by heating a solution of the starch until a stable paste formed for a few seconds. The samples showed low water solubility (0.13 M1 and 0.23 M2 g / 100 mL) and a high water retention capacity (280.3 and 391.7% at 60 ° C) compared to 226% of the cassava and 162.8% for sago: which makes it a good quality starch. The gel pastes when subjected to a % processing of light at 650 nm in a Thermo Evo 201 UV spectrophotometer was (3.4 and 4.1%) respectively. Comparing these characteristics found in this tuber with other sources of starch commonly used in the industry, it is concluded that it can be used as a promising alternative in the food industry; In addition to having a high amylose content, it is a resistant starch, providing physiological health benefits.
Diseño, síntesis y actividad biológica de péptidos antimicrobianos catiónicos
(Universidad Santiago de Cali, 2019) Vargas Carabalí, Lina Fernanda; Oñate Garzon, Lina Fernanda
Antibacterial resistance to conventional antibiotics is an emerging problem worldwide. This urge to develop alternative antibiotics has motivated researchers to look for new molecules to overcome this resistance problem, such as antimicrobial peptides (PAMs). PAMs are important effector molecules of the innate immune system, which is the main defense mechanism for most living organisms. Alyteserin 1c, is an antimicrobial peptide secreted in the skin of the toad Alytes obstetricans characterized by having a net charge +2 (WT). Based on the WT peptide, an analogue (ΔM) was developed, which presents a +5 net charge as a result of different amino acid substitutions in the polar face of the Alyteserin 1c helix. Both peptides were synthesized using the solid phase peptide synthesis method. The yield of the unpurified synthesis was 29.7% for each peptide. After the process of purification by reverse phase high efficiency liquid chromatography, the yield was 4.95% for the WT and 13.2% for the ΔM. Subsequently, the purity of the peptides was verified using the Bruker Daltonics MALDI-TOF mass spectrophotometer, which was 95% in both peptides. Regarding the antibacterial activity, the WT peptide exhibited a minimum inhibitory concentration (MIC) of 62.5 μM and 250 μM, against the bacteria Staphylococcus aureus and Salmonella typhimuriom, respectively, on the other hand, with the ΔM MICs were obtained from 125 μM and 62.5 μM, for both strains respectively. Regarding the hemolytic activity of the peptide WT and ΔM, it was observed that the minimum hemolytic concentration is 62.5 μM in each one.
Evaluación de las propiedades fisicoquímicas del almidón termoplástico en presencia de ácido láctico
(Universidad Santiago de Cali, 2019) Pastrana Gutiérrez, Isabel
The thermoplastic starch was modified with lactic acid in the molten state, using two types of starches (achira and potato) and two different plasticizers, glycerol and sorbitol. These components were used in a 70:30 ratio (starch: plasticizer) and the addition of acidic agent was carried out in portions of 3, 6 and 9% by weight. These mixtures were obtained in a torque rheometer at 130 ° C for 10 min. The thermal characterization performed showed greater stability for the samples of TPSL> TPS, the values in the temperature of degradation varied between 230 ° C to 280 ° C, while for the thermal transitions corresponding to the temperature of gelatinization and fusion they showed a decrease in the crystallinity for the samples with 6% oleic acid, these results were consistent with the morphological analysis. The results derived from the oscillatory rheological analysis had a viscous behavior in the TPS samples, while the presence of the acid generated viscous to elastic transitions. Finally, a reduction in the hydrophilicity of the modified TPS was evidenced because some of the samples increased the value of the contact angle by up to ~ 46%.
Estudio de las interacciones del péptido Alyteserin 1C y de su análogo catiónico con modelos de membranas
(2019) Sanchez Arias; Kevin; Oñate Garzón; José Fernando
Antimicrobial peptides are part of the innate immunity of most living things and have antimicrobial activity against bacterium, viruses, fungi and parasites. Their cationic characteristics make them selective towards bacterial membranes with anionic surfaces.
Polímeros de coordinación de plata(I), cobalto(II) y manganeso(II) con PVA inmovilizado en quitosano depositado en aluminio y vidrio erosionado
(Universidad Santiago de Cali, 2019) Díaz Canchimbo, Viviana Maria; Avila Torres, Yenny Patricia
The aim of this research work was the development of low and medium molecular weight chitosan (Q) membranes embedded with polyvinyl alcohol, which were enriched with the metal ions of: copper (II), cobalt (II), manganese ( II) and silver (I) as new bactericidal materials. The method used was electrospinning, establishing optimal deposit conditions (DC 11 cm, VB 0.01 mL / hr, V 16 Kv, T 70 min and 16% PVA) on two aluminum surfaces and eroded glass. The antibacterial activity was carried out against Staphylococcus aureus and Eschericia Coli by comparing the activity in solution and its corresponding supported membrane. The method used was Kirby - Bauer modified discs, implementing as positive controls amoxicillin at 25000 ppm and the metal salts at 50000 ppm by each transition metals. The preliminary results show bactericidal and bacteriostatic activity mediated by the nature of the metal ion, further studies are proposed in order to understand the diffusion process and the effect on the stability of the coordination polymers with respect to biological activity.
Estudio de la micota ambiental de tres edificios de la universidad Santiago de Cali, Colombia
(Universidad Santiago de Cali, 2019) Lasso Ceballos, Ana María; Caicedo Bejarano, Luz Dary
The quantification of the environmental load in the indoors of the buildings (1, 3 and 4) of the University Santiago De Cali, Colombia and in their corresponding responses to the results to obtain a load value. A volumetric method and Dicloran Bengal chloramphenicol (DRBC) and CHROMagar Cándida culture media were used for sampling. The identification of the fungi was carried out based on macro and microscopic characteristics. Parallel to the sampling the relative humidity and temperature of each space studied was determined. This study was conducted for quarterly periods during twelve months (March 2018 to march 2019). In the study of environments of the three buildings was detected a total of 82393 colony forming units (CFU/m3) of which 60053 CFU/m3correspond to indoor environment. The results obtained showed that it was not possible to establish a correlation between the temperature and the CFU/m3 found inside the three buildings, but for the relative humidity in relation to building 4.The genera Cladosporium, Fusarium, Penicillium and Aspergillus were identified as the most prevalent allergenic fungi in this study. The results in the quantification revealed that the fungal burden exceeds the limits accepted for internal environments (500 CFU/m3) according to the World Health Organization (WHO).
Estudio estructural y supramolecular por medio de difraccion de rayos x y superficies de hirshfeld del compuesto ácido 2-(e)-((4-hidroxifenil) diazenil) benzoico
(Universidad Santiago de Cali, 2019) García Carreño, Camila Andrea; D´Vries Arturo, Richard Fernando
The crystalline structure of 2-(E)-((4-hydroxyphenyl) diazenil) benzoic acid compound, was resolved by means of the intrinsic phase method using single-crystal X-ray diffraction data. It was find that the molecule crystallizes in the ortorombic crystalline system with Pbca space group. Within its unit cell there are four molecules per asymmetric unit, which are molecular conformers and form beta helix arrangement along [0 1 0] direction. Hirshfeld surface calculation was performed to determine the intermolecular interactions that contribute the most to crystalline packaging. It was possible to observe that the main contribution to the general surface is given by the contacts H…H (34.8%), followed by interactions O…H/H…O (27%) and C…H/H…C (18.6%). The energy interaction networks were calculated at a HF/3-2 level, allowing to quantify the high participation of dispersion interactions (-57.5 kJ/mol) in the formation of crystalline packaging. Keywords: crystallography, Hirshfeld surfaces, X ray, cell unit, intrinsic phase
Biota fúngica en fosas nasales de trabajadores de tres edificios de la universidad Santiago de Cali y su relación con síntomas de alergias respiratorias
(Universidad Santiago de Cali, 2019) Castillo Tellez, Beatriz Angélica; Caicedo Bejarano, Luz Dary
Allergenic fungi enter through the air to nostrils and may be responsible for some respiratory allergies. The objective of the present study was to determine the relationship between allergies diagnosed and undiagnosed according to the results obtained from the adapted survey of ISAAC (International Study of Asthma and Allergies in Childhood) and the fungal load of the nostrils of workers of three buildings of the Santiago de Cali University. The samples taken from the nostrils were made with sterile swabs and the subsequent sowing was done on Sabouraud dextrose agar, sunflower seed agar and Candida CHROMagar. In 100% of the workers (126) who participated in the study, allergenic fungi were isolated. The highest prevalence of allergenic fungi in the workers of the different buildings was obtained by the workers of block 1, finding significant statistical differences, for the genus Fusarium sp, (p=0,0018 Kruskal Wallis) and Penicillum sp, (p=0,0080 Kruskal Wallis), but not for Cladosporium sp, Alternaria sp and Aspergillus sp; likewise, the biggest fungal load was presented by the workers of this building. When analyzing the fungal 6load found in the nasal passages and its relation with the presence of symptoms of respiratory allergies, it was established that there was significant statistical association with the genus Fusarium sp (p=0,0430 Wilcoxon Mann Whitney) and not for Cladosporium sp (p=0,1918, Wilcoxon Mann Whitney) which was the most frequently isolated genus.
Caracterización molecular de aislados de Enterobacter cloacae resistentes a carbapenemes portadores de los genes blaKPC, blaNDM, blaVIM y blaOXA-48, provenientes de la red de laboratorios públicos y privados del Valle del Cauca
(Universidad Santiago de Cali, 2019) Guerrero De La Cruz, Daniela Andrea; Restrepo Falco, Aura
The production of beta-lactamase enzymes by some species of enterobacteria is a serious public health problem because it causes the loss of therapeutic efficacy to beta-lactam antibiotics, which are currently used as the first alternative for the management of infections caused by Gram-negative bacteria. In this work, the molecular characterization of 32 phenotypically resistant Enterobacter cloacae clinical isolates of cephalosporins and carbapenemes, from a private and a public entity, located in the city of Cali, was carried out. In order to detect the genes involved in resistance to these antibiotics, the polymerase chain reaction technique was used to amplify the genes encoding carbapenemases: KPC, NDM, VIM and OXA-48. It was obtained that 50% (16/32) of the isolates are carriers of the blaKPC gene, while the presence of blaNDM, blaVIM and blaOXA-48 genes was not detected. The results of this study indicate that the allele that circulates in the isolates is KPC-2 and that they are not genetically related. The results indicate that 81% of patients infected with the enterobacteria carrying KPC carbapenemase come from private medical centers, 81% are male and 44% of those affected are older adults (60 years and older). Knowing the genes involved in carbapenemes resistance in these clinical isolates is an important step towards the development of specific strategies to prevent the spread of this multi-drug resistant pathogen in the municipality of Cali.
Diseño de un sistema de gestión ambiental para la empresa Análisis Ambiental S.A.S., basado en la norma NTC 14001: 2015
(Universidad Santiago de Cali, 2019) Mosquera Mosquera, Gloria Stephanía; Angulo Piñeros, Nathalia; Garzón García, Guillermo; Robles Pardo, Edilberto
La empresa ANALISIS AMBIENTAL S.A.S. presta servicios de Ingeniería Ambiental y Sanitaria que incluyen asesorías en plantas de tratamiento de aguas, monitoreos en agua, aire y suelos; como también servicios de análisis de Laboratorio fisicoquímicos y microbiológicos. Estos servicios generan impactos sobre el medio ambiente, y siendo conscientes de ello, se ha diseñado un Sistema de Gestión Ambiental basado en la Norma NTC - ISO 14001 de 2015 para conocer su naturaleza, y de esa forma mitigar y/o controlarlos de manera efectiva.
Caracterización fisicoquímica, sensorial y micológica del kéfir de agua en jugos de frutas tropicales
(Universidad Santiago de Cali, 2019) Pedraza Morales, Yiham Stephanie; Ramírez Navas, Juan Sebastián
Kefir contains beneficial properties that promote health, which is why microorganisms isolated from kefir are studied for the development of functional foods. In the present study, the physicochemical, sensory and growth characteristics and phenotypic characterization of fungi present in water kefir in tropical fruit juices were determined. This experiment was done under a factorial design corresponding to the combination of two factors with three blocks and three replicas: type of substrate (pear juice, passion fruit and water with panela) and sucrose concentration (25%, 50% and 100%). The response variables were: acidity, pH, Brix degrees. For the microbiological analyzes, decimal dilutions were made, they were counted on the second and fifth day of incubation. The identification was made with API 20C and Vitek 2 (Biomérieux). Three yeast strains found from higher to lower number of CFU/mL Saccharomyces cerevisiae 1, Saccharomyces cerevisiae 2 and Zygosaccharomyces spp were finally isolated with the sensory analysis it was determined that the passion fruit drink was the one that had the best acceptance and preference on the part of consumers.
Generación de carbón activado a partir del residuo de cosecha de Ananas comosus para la remoción de metales pesados Plomo (Pb+2) y Cadmio (Cd+2)
(Universidad Santiago de Cali, 2019) Nidia Marcela Velásquez Varón; Zambrano Salgado, Yesid Fabián
In this study, activated charcoal was prepared from the leaves of Ananas comosus; by chemical activation, from two different pyrolysis temperatures (500 ° C and 600 ° C) and different concentrations of activating agent (20%, 40%,42,5% and 60%) in a 6: 1 impregnation ratio, with which yield percentages were obtained which varied from 47.15% to 54.82%. The surface chemistry of the coals was studied by FTIR infrared spectroscopy. The adsorbent properties of heavy metals (Pb2 + and Cd2 +) were measured from aqueous solutions, obtaining a removal percentage above 80% for both solutions. The adsorption isotherm was performed, in which the linearized experimental data fit very well to the Langmuir adsorption isotherm model, where the chemotherapy of lead and cadmium heavy metals is produced from the aqueous solutions.
Evaluación del efecto de la plata coloidal, en la remoción de escherichia coli y la obtención de un agua salubre a partir de filtros caseros
(Universidad Santiago de Cali, 2019) Carabalí Ramos, Juan José; Pérez Vidal, Andrea; Rivera Sánchez, Sandra Patricia; Pérez Vidal, Andrea
Water is a natural and indispensable resource for life, for this reason it is very important to supply it, in a drinkable, affordable and good quality way to be supplied to the community in general, fulfilling the physicochemical characteristics, Microbiological and sanitary concept, established in the standard, Resolution 2115 of 2007. However, despite drinking water being a right, many communities, especially rural ones, still do not have access to quality water; presenting Diarrheal Watered Diseases (EDA) and Foodborne Diseases (ETA) which are induced by the intake of unhealthy water, among other factors that help to spread the disease. For this reason, alternative techniques and / or processes for water treatment have been sought, among which techniques such as home filtration systems for obtaining drinking water have been highlighted. These filters are composed of various materials, such as colloidal silver, activated carbon, which possess antimicrobial potential and / or retention capacity of substances and microorganisms. In order to mitigate the EDA and ETA, the REPLACOL microenterprise developed and provided the EKOFIL filtration systems impregnated with colloidal silver, and was sought as: Objective: To evaluate the effect of colloidal silver on the removal of Escherichia coli in obtaining a safe water from homemade filters. Methodology: The process of water purification was carried out in the facilities of the Santiago de Cali University, subjecting the home filters to certain concentration of synthetic water, that is; 103, in accordance with the inoculation provided from E. coli strain ATCC 25922 employed by membrane filtration (FM) for the diagnosis of water quality at certain times of 1, 8 and 24 hours of silver contact with filtered effluent and residual silver. Through statistical analysis (Kruskal-Wallis and post anova) they were obtained as: Results: The determination of the best contact time in conjunction with the best filtration rate by home filters therefore determined the filter defined as E 1.0 which managed to remove up to 3.9 logarithmic units from the initial synthetic water. Conclusion: The efficiency of removal of the E 1.0 filter with respect to the other systems is observed, determining that the best contact time occurs at a time of 8 hours with a filtration rate of 1L / h where the removal reaches up to 100 % of effectiveness
Caracterización de bacterias asociadas a la rizosfera de plantas de yuca (manihot esculenta crantz) sometidas a estrés por déficit hídrico
(Universidad Santiago de Cali, 2019) Galindo Espinal, Diana Marcela; Zapata Gualtero, Tatiana
Cassava (Manihot esculenta Crantz) is one of the most important energy sources in the tropical and subtropical regions of the world. During the last years, its production has resulted in extreme environmental conditions. Plant growth promoting rhizobacteria (PGPR) are a type of bacteria that colonize the roots and a symbolic relationship with the plant, favoring their growth and resistance even in conditions of abiotic stress. In the present study, 124 bacterial components of cassava plants M. esculenta are characterized, of which 26 rhizobacteria are identified in plants over a period of 20 days. The analyzes of the abundance of the most predominant genera are: Bacillus, Achromobacter, Pseudomonas, Sphingomonas and Streptomyces. These results are important for bacterial communities such as cassava cultivation and its relationship with the response of plants to abiotic stress conditions.
Comparación de dos técnicas para la captación de esporas fúngicas ambientales en un campus universitario
(Universidad Santiago de Cali, 2019) Delgado Ñañez, Cristhian; Caicedo Bejarano, Luz Dary
The concordance of two fungal spore uptake techniques in the environments of three buildings of the Santiago de Cali University campus was evaluated. The gravimetric method of spore uptake was compared with the Omeliansky formula and the volumetric method (Air Ideal 3P). The environmental sampling was carried out quarterly for a period of 12 months using Dicloran Rose Bengal Chloramphenicol agar (DRBC). 64497 CFU / m3 were accounted for, of which 34930 CFU / m3 were isolated with the volumetric method and 29567 CFU / m3 with the gravimetric method. No significant statistical differences were observed with the two techniques used (p = 0.0739), with a significance level α = 0.05. The predominant genera obtained with both methods were Cladosporium, Penicillium, Aspergillus and Fusarium, which suggests that although the gravimetric method is not as efficient as the volumetric method, it is reliable for estimating the degree of environmental fungal contamination in a similar way to the volumetric method.
Evaluación de la remoción de salmonella spp. a partir de dos calidades de aguas sintéticas tratadas con filtros caseros
(Universidad Santiago de Cali, 2019) Castaño Hincapié, Ana Valentina; Rivera Sánchez, Sandra Patricia
Water is the main resource that abounds in the Earth. It is not renewable and has great importance, both for human consumption and for the rest of living beings. It is used for domestic uses or other purposes. The low coverage of aqueduct and sewerage in rural areas of Colombia, added to the fact that the implementation of potabilization in these areas entails a high cost, have favored the creation of new technologies that imply minor resources and accessibility, such as the homemade filters.
Cuantificación por HPLC de Trans-resveratrol en la uva Isabella (vitis labrusca) cultivadas en la Unión-Valle del Cauca
(Universidad Santiago de Cali, 2019) Ospina Ramírez, Stephanie; Castillo Ayala, Jorge Enrique
The Isabella grape (Vitis labrusca) is a variety that has been optimally adapted to the climatic conditions of La Unión-Valle del Cauca, and has had good acceptance in local markets. Currently the grape is consumed fresh and its phenolic content is unknown. According to epidemiological studies, the presence of bioactive compounds with antioxidant capacity such as trans-resveratrol, can exert a protective effect against some diseases such as cancer and cardiovascular disorders. For this reason, with this work the quantification of the trans-resveratrol present in the epicarp of the Isabella grape (Vitis labrusca) grown in La Unión-Valle del Cauca is performed, using high efficiency liquid chromatography (HPLC) with ultraviolet detector. It will ensure that the method to follow complies with the evaluation parameters (linearity, precision and accuracy). It is also intended to contribute to a methodological proposal for the extraction (liquid-liquid with acidified methanol) and purification of trans-resveratrol.
Validación secundaria del método de filtración por membrana para la detección de microorganismos indicadores de contaminación en muestras de agua de uso farmacéutico
(Universidad Santiago de Cali, 2019) Guarnizo Mejía, Mariana; Nieto Ramírez, Luisa María
Purified water is supplied in the manufacture of non-parenteral products in the pharmaceutical industry. This input requires a microbiological analysis due to the susceptibility of microbial contamination in the distribution process, thanks to the presence of biofilms, among other factors. The filtration by membrane allows detecting the causes of said contamination and for this reason it is important to verify this methodology in the industry so that it can be used as a method of quality control in the plant located in the urban area of Cali. The present work was carried out in the pharmaceutical company Sanofi Cali, where the challenge organisms used in each stage of the process were evaluated, respectively the strains ATCC (American Type Culture Collection) Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027. The stages of the process were a) inoculum standardization, b) productivity and selectivity tests of the culture media (Plate Count Agar, Chromocult and Cetrimide), c) development of the secondary validation technique of the membrane filtration method. The comparison of the results obtained by two analysts was included during the validation process. The effectiveness of validation for S. aureus and E. coli and non-effectiveness in P. aeruginosa was demonstrated.
Susceptibilidad a los antimicóticos de levaduras aisladas de sistemas de aguas de la ciudad de Santiago de Cali, Colombia
(Universidad Santiago de Cali, 2019) Osorio Vanegas, Lizeth Stefania; Caicedo Bejarano, Luz Dary
Water systems become a high capacity of microorganisms. The objective of this work was to evaluate the susceptibility to the antifungals of yeasts present in the wastewater and natural waters of the city of Cali. Samples were collected from two types of water: potable (Río Melendez, Puerto Mallarino) and residual (Canal Sur, Planta de Tratamiento Cañaveralejo-PTAR). Yeasts were isolated in sabouraud and CHROMagar Candida in the commercial identification with API 20 C AUX (BioMérieux) and in the sequence analysis of the ITS1- 5.8S-ITS2 region. The minimum inhibitory concentration (MIC) of fluconazole and amphotericin was determined by the microdilution method, and the influence of some physicochemical parameters and heavy metals was correlated with the possibility of yeasts and antifungals. With the phenotypic tests, the 15 yeast species are identified and the molecular biology techniques six species, the morphotype of the property that can belong to the new species of the genus Candida. 100% of the strains were sensitive to Amphotericin B, and 44.3% were sensitive, 19.23% were sensitive dose-dependent (DDS) and the remaining 25% were resistant to fluconazole. Finally, a correlation has been found between the minimum inhibitory concentration, some physicochemical parameters and the concentration of heavy metals on the antifungal sensitivity of yeasts associated with aquatic systems in the city of Cali.

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