Medicina Veterinaria
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Item Manejo terapéutico para ehrlichiosis monocítica canina: revisión sistemática(Universidad Santiago de Cali, 2024) Fernández Rueda, María del Mar; Vásquez González, Michelle; Gil Aranzazu, César Hernando (Director)Canine Monocytic Ehrlichiosis (CME) is an infectious disease caused by the gram-negative bacterium Ehrlichia canis, transmitted by the tick Rhipicephalus sanguineus. This disease affects dogs of any age, breed, or gender and is prevalent in various regions of the world, including Colombia. Currently, there are several therapeutic approaches to address CME, including both traditional antibiotic treatments, with doxycycline being the most effective, followed by options such as rifampicin, minocycline, and azithromycin, as well as innovative alternative therapeutic options like plant extracts and homeopathic therapies. Additionally, for disease prevention, strategies are employed to control the biological vector, such as the use of essential oils and biological agents. This review aims to provide veterinary professionals with updated information on best practices for the prevention and treatment of CME, in order to reduce its prevalence and increase the life expectancy of affected dogs.Item Uretrostomía pre púbica en felinos domésticos como una alternativa de tratamiento quirúrgico para obstrucciones uretrales post uretrostomia perineal, revisión sistemática(Universidad Santiago de Cali, 2024) Zorrilla Aragón, Juan Camilo; Pipicano Rodríguez, Isabella; Londoño Rojas, Andrea (Directora)It is taken into account that male felines, due to their lower urinary tract anatomy, have a greater predisposition to suffer from obstructive diseases that are directly associated with sediments, stones, tumors, stenosis, among others, which has led veterinary doctors to opt for Surgicalalternatives to improve quality of life, perineal urethrostomy is a treatment option, however it usually presents some complications, which result in surgical reinterventions such as prepubic urethrostomy. The general objective of this literary review is to identify how prepubic urethrostomy can be an alternative to surgical treatment for the correction of a failed perineal urethrostomy, where indexed literature, review articles, databases such as scopus, sciencedirect, among others, will be reviewed; where post-pelvic urethrostomy is suggested, as the first alternative to correct the initial failed intervention or with complications, but this alternative is difficult to access since it must also intervene in the orthopedic area, prepubic urethrostomy is an option In practice, although it may present complications such as scald dermatitis, incontinence and stenosis, it is usually used as a definitive treatment in case the obstruction persists and treatment is exhausted due to the complications that may arise.Item Variación en los niveles de cortisol en felinos domésticos asociado a enriquecimiento ambiental: revisión sistemática(Universidad Santiago de Cali, 2024) Chacón Viteri, Juliana; Díaz Caycedo, Mariana; Guarín Patarroyo, Camilo Ernesto (Director)Cortisol is a hormone produced by the adrenal glands and is known as a stress marker or “stress hormone” in both humans and animals; the domestic cat is one of the most sensitive animals to changes in their environment (internal or external), being subjected to stressful situations more easily. The objective of this review project is to gather information on the analysis of cortisol levels in shelters with domestic cats; cortisol is a substance produced by the adrenal glands, it is a crucial hormone in the response to stress that also participates in the regulation of metabolism and has immunological functions. In accordance with the above and considering that cats have undergone a different domestication process than dogs (where they have tried to keep their instincts intact), it is important to evaluate whether the environment provided in shelters influences the levels of the aforementioned hormone. It is proposed as a research methodology to gather and analyze scientific publications that gather information on the proposed topic; it is expected that the results of this review will help to identify whether the environment and lifestyle of cats can favor their response to stress by directly relating to cortisol levels.Item Evaluación del bienestar animal en una fundación al oriente de Cali, mediante el marco de las cinco libertades(Universidad Santiago de Cali, 2025) Márquez Orozco, María Camila; López Reyes, Alejandra; Guarín Patarroyo, Camilo Ernesto (Director)Animal welfare is defined as the state of harmony between the environment and the animal's response. For its evaluation, five freedoms have been established as the foundation for respect and progress in this field. The objective of this study was to evaluate welfare conditions in a foundation according to the standards of the five freedoms framework. The methodology involved creating a checklist to assess animal welfare at the Comedog Foundation during the current year. This evaluation system assigns a maximum score of 20% to each freedom, totaling 100%, which standardizes the measurement of animal welfare across different contexts. The results showed that the foundation achieved 10% in "Free from hunger, thirst, and malnutrition" (50% of the maximum score for this freedom), 10% in "Free from fear and distress" (50%), 13% in "Free from physical and thermal discomfort" (65%), 16% in "Free from pain, injury, and disease" (80%), and 12% in "Free to express natural behavior" (60%). Overall, the foundation scored 1,325 points, equivalent to 66%, classifying it within the High Animal Welfare level. The results indicate that, while the foundation maintains an acceptable level of animal welfare, there are specific areas that could be improved to achieve a more comprehensive fulfillment of the five freedoms.Item Eficacia y rapidez en la cicatrización de heridas con miel de abejas en caninos y felinos. Revisión sistemática(Universidad Santiago de Cali, 2025) Monroy Morales, Karen Andrea; Restrepo Santamaría, Nicolle; Guarín Patarroyo, Camilo Ernesto (Director)The use of honey in veterinary medicine has been experiencing a boom in recent years. However, research on the benefits of this substance is recent and scarce in relation to others. The present review aimed to compile and analyze the most recent articles available, in order to verify the efficacy of honey in the treatment and recovery of wounds in dogs and cats. To select the articles, parameters of language, year of publication and concordance with the main objective were established. Keywords such as honey, wound, dogs, cats and healing were used. The search was performed in four databases. The selected publications were systematized in a matrix, which allowed for a rigorous analysis of the data, as well as a The selected publications were systematized in a matrix, which allowed a rigorous analysis of the data, as well as contrasting and classifying the information. The results indicate that honey possesses physicochemical properties with anti-inflammatory, antibacterial and regenerative potential, which makes it a therapeutic option for the treatment of wounds. Although some commercial pharmaceuticals may show greater efficacy in certain aspects, honey stands out as an economical and effective alternative. Moreover, when combined with other substances, its therapeutic effect is enhanced, improving results compared to its isolated use. In addition, when combined with other substances, its therapeutic effect is enhanced, improving the results compared to its isolated use. Even so, honey alone has been shown to be effective in the healing and scarring of lesions, providing positive results in different veterinary contexts. This work suggests that future research is needed to validate with greater scientific rigor its use and explore its potential in the development of innovative treatments in veterinary medicine.Item Hallazgos clínicos, diagnóstico y evolución de casos atendidos de primates no humanos en un Centro de Atención y Valoración de Fauna Silvestre del Suroccidente colombiano, 2023-2024(Universidad Santiago de Cali, 2025) Velasco Vargas, Carol Gisel; Figueroa Henao, Luis Alejandro; Bermeo Sierra, Marta Liliana (Directora)The wildlife rescue center are entities in charge of ensuring the health and welfare of the animals that get into their facilities, being victims of different negative situations such as trafficking and illegal possession, burns from forest fires, injuries of anthropic origin, among others. Non-human primates are a widely trafficked taxonomic group, because they extract them from their natural habitat to keep them as pets, affecting their physical, mental and social state. Documenting this problem at a qualitative and quantitative level it is an useful tool to determine which are the most affected species, the main clinical findings with which they enter, their diagnosis and evolution. This study presents the data collected from the clinical cases of non-human primates treated during the years 2023 and 2024, in the wildlife rescue center DAGMA, located in Cali, Valle del Cauca.Item El enriquecimiento ambiental y su efectividad en zoológicos y centros de recepción de fauna silvestre en Latinoamérica: Revisión sistemática(Universidad Santiago de Cali, 2025) Mina Escobar, Melisa Andrea; Guzmán Salinas, Tatiana; Casilimas Cabal, Jorge Alejandro (Director)Environmental enrichment is a fundamental, necessary and valuable resource for all animals under human care, since it contributes to improve their health and welfare, through the implementation of activities or modifications in the environment that allow an individual to express behaviors typical of its species. Wild animals under professional care require to a greater extent the application of different types of enrichment such as nutritional, sensory, structural and social, in order to reduce their stress levels, and consequently, reduce the presentation of stereotypies and infectious diseases due to immunosuppression. This paper focuses on a literature review on the mentioned subject, investigating the environmental enrichment protocols implemented by different zoos and wildlife reception centers in Latin America, contrasting the degree of effectiveness and the impact they have had on the welfare of the animals. Methodologically, information was collected from scientific articles obtained through different databases and then the information was analyzed to identify in which taxonomic groups more research is reported, which types of environmental enrichment are the most implemented, which strategies or enrichment agents are the most used and which of them have had a positive response from the animals. The purpose of this work is to serve as a reference for the design of environmental enrichment protocols or programs, and additionally, to identify the gaps in knowledge on the subject.Item Uso de la acupuntura tradicional y electro acupuntura como tratamiento para el manejo del dolor en caninos y felinos: Revisión sistemática(Universidad Santiago de Cali, 2025) Cobo Capote, Jessica Juliana; Delgado Diago, Geraldine; Guarin Patarroyo, Camilo Ernesto (Director)Acupuncture and electroacupuncture have been proposed as effective complementary techniques for pain management in veterinary medicine. However, in Colombia, their use has been limited due to the lack of local scientific evidence and the scarce specialized training in these techniques. The aim of this study was to evaluate the effectiveness of acupuncture and electroacupuncture in canines and felines, comparing them with conventional medicine in the treatment of pain. A systematic review of the scientific literature was performed, reviewing studies published between 2019 and 2024. Studies were selected from databases such as PubMed, ScienceDirect, Google Scholar, Scopus and Sage Journals, using inclusion criteria that focused on research on acupuncture applied to pain management in pets and studies conducted with non-conventional animals. Papers that did not meet the methodological standards required for this study were excluded. The results revealed that acupuncture and electroacupuncture are effective and promising techniques for the treatment of chronic, neuropathic and post-surgical pain in canines and felines. These therapies were successful in most studies in reducing pain through the stimulation of specific anatomical acupuncture points. However, the need to develop more research in Colombia to study this complementary medicine in a local context was highlighted, since the extent of its efficiency is still not very clear and there is a need for clinical studies with a more representative sampling.Item Enfermedad periodontal en perros: Identificación de etapas, tratamiento y prevención: Revisión sistemática(Universidad Santiago de Cali, 2025) Salcedo Alarcón, Nallely; Chaves Obando, María Camila; Londoño Rojas, Andrea; Guarin Patarroyo, Camilo Ernesto (Director)Periodontal disease (PD) is one of the most frequent causes of dental disease in dogs, because it is a pathology produced by an accumulation of bacterial plaque, which initially produces gingivitis and later irreversible damage such as periodontitis. Periodontal disease is an alteration that is normally not considered by the guardians, causing irreversible damage at the oral level and provoking cardiac, hepatic and/or renal diseases. Taking this into account, it is important to be able to evaluate in which stage of the disease the animal is, to be able to give adequate treatment and then continue with prevention to avoid future relapses and diseases. It is proposed as a methodology to compile and review scientific publications that gather information about periodontal disease, its stages, treatment and prevention. This research article aims to identify the main causes of periodontal disease, identify the stages that can occur in dogs and offer treatments according to their stage; it also highlights the importance of prevention of periodontal disease to avoid complications later.Item Importancia clínica en el diagnostico de mastocitoma cutáneo canino: Revisión sistemática(Universidad Santiago de Cali, 2025) Calderón Vallejo, Evelin Tatiana; Ocampo Torres, Jessica; Guarin Patarroyo, Camilo Ernesto (Director)Mastocytomas are neoplasias generated by the abnormal proliferation of mast cells, classified as the most common hematopoietic neoplasia in dogs. There are breeds that are predisposed to this pathology and it is usually associated with geriatric animals. There are no studies that accurately demonstrate incidence according to sex. This work reviews the literature on the diagnosis of cutaneous mastocytoma in canines, its casuistry, clinical signs, treatment and diagnostic methods, in which we find the Patnai method to classify the histological stage and the kiupel method that allows us to know the degree at which it can remain (high or low).Item Impacto de la proteína animal en la salud y bienestar de perros (Canis familiaris) y gatos (Felis catus): Revisión sistemática(Universidad Santiago de Cali, 2025-10-09) Echeverri Gil, Melany Tatiana; Ospina Varón, Carlos Mario (Director)Nutrition is a fundamental aspect of dog and cat health, especially in relation to the quality and quantity of animal proteins in their diet. The aim of this monograph was to analyze the impact of these proteins on the well-being and physiological functioning of these species through a systematic review of scientific literature published between 2020 and 2025, following the PRISMA guidelines, where indexed databases were consulted, applying previously defined inclusion and exclusion criteria. Of a total of 247 records identified, 50 were included in the qualitative synthesis. The review identified important differences between species, both in their nutritional requirements and in their physiology and protein metabolism. Cats, as obligatory carnivores, need higher levels of specific amino acids such as taurine and arginine, while dogs, although more flexible as facultative carnivores, also show better physiological responses to diets containing animal protein. Common protein sources, their digestibility, biological value, and impact on the metabolism and overall health of these species were analyzed. The results indicate that diets adequate in animal protein improve multiple functions in the body, such as immune response, muscle mass maintenance, development, growth, and the prevention of clinical disorders. Conversely, protein imbalances can have significant consequences such as weight loss, digestive problems, and metabolic disorders, thus demonstrating their importance in overall health and making them a relevant clinical tool in veterinary practice.Item Prevalencia de Parvovirosis en caninos previamente inmunizados en una Clínica de Santiago de Cali en 2023(Universidad Santiago de Cali, 2025-10-13) Delgado Chimunja, Yelisabel ; Diez López, Melissa; Guarín Patarroyo, Camilo (Director); Saavedra, Camila (Directora)Canine parvovirus (CPV) is a disease caused by the DNA virus of the species Carnivorous Proto parvovirus 1, which affects canines (Canis lupus familiaris) of all ages. This study aims to identify the prevalence of CPV in previously immunized canines that attended the veterinary clinic in Cali, year 023. A descriptive, observational, cross-sectional design will be used with a sample of 80 immunized patients to evaluate the effectiveness of the vaccines. , vaccination schedules and associated factors. The data obtained are compared with previous studies to generate useful conclusions for veterinary practice, contributing to the optimization of parvovirus prevention strategies.Item Efectos de la nutrición mineral sobre la integridad óseas en cerdas durante la gestación y lactancia(Universidad Santiago de Cali, 2025-11-03) Tobar Pino, Sonia Viviana; Gutiérrez Gómez, Juan David (Director); Cabrera Matajira, Carlos Emilio (Director)La revisión analizo la problemática de los efectos de la nutrición mineral sobre la integridad Oseas en cerdas durante la gestación y lactancia los cuales afectan la salud maternal, la mineralización ósea, la calidad del calostro y la viabilidad de los lechones. Tuvo como objetivo analizar las causas nutricionales relacionadas con la aparición de enfermedades óseas en cerdas asociadas a deficiencia de minerales, los efectos de diferentes estrategias nutricionales sobre el metabolismo, la inmunidad y el rendimiento reproductivo de las cerdas modernas. Se aplico la metodología PRISMA para la búsqueda y selección de estudios recientes que evaluaron la suplementación con minerales, vitaminas, probióticos, nucleótidos, ácidos grasos. Fibras y compuestos funcionales en dietas para cerdas gestantes y lactantes. Los resultados abordaron que la restricción de calcio y fosforo afecto la mineralización ósea, mientras que su adecuada suplementación mejoro la salud esquelética y la calidad del calostro. Los probióticos y nucleótidos aumentaron la capacidad antioxidante, el consumo de alimento y la vitalidad de los lechones. La reducción de la relación ω6:ω3 y la inclusión de fibras disminuyeron el estrés oxidativo y térmico, mejorando el confort materno. La vitamina D3 y el selenio orgánico mostraron mayor biodisponibilidad y eficiencia reproductiva que los inorgánicos. Se concluyo que la nutrición materna desempeño un papel determinante en la productividad y la supervivencia neonatal. Una nutrición con una formulación precisa que combine minerales esenciales, vitaminas y aditivos funcionales permitió optimizar la eficiencia metabólica, la respuesta inmune y el bienestar general de las cerdas y sus lechones en sistemas de producción intensiva.Item Contaminación con Fumonisinas y Aflatoxinas en maíz para porcicultura: implicaciones en salud pública(Universidad Santiago de Cali, 2025-11-04) Rassa Pinto, Gabriela; Guarín Patarroyo, Camilo Ernesto (Director); Cabrera Matajira, Carlos Emilio (Director)Mycotoxins, specifically fumonisins and aflatoxins, have represented a significant problem for swine production and public health, as they are frequently found in corn and its derivatives. The purpose of this study was to critically analyze the most recent scientific literature related to the proliferation of mycotoxins in corn, their effects on swine health and productivity, the risks they pose to human consumers, and proposed mitigation strategies. To this end, a systematic review was conducted of twenty-five articles published between 2019 and 2025 in academic databases, which included both experimental and observational studies. The information obtained was organized into a matrix and subsequently classified into different thematic areas. The findings showed that the combination of high humidity and high temperatures, coupled with poor post-harvest handling, favors the proliferation of mycotoxins in grains. Furthermore, in pigs, prolonged exposure to these substances caused immunosuppression, hepatotoxicity, reproductive disorders, and a decrease in production indicators, even when the doses were subclinical. Regarding human health, bioaccumulation was identified in frequently consumed tissues, and potential carcinogenic and mutagenic effects were noted, although epidemiological evidence remains limited. Finally, mitigation measures highlighted included the use of resistant varieties, the use of adsorbents in the diet, the proper application of agricultural practices, and the development of rapid detection technologies, the effectiveness of which still needs validation under field conditions. Thus, mycotoxins constitute a multidimensional problem that affects animal productivity, food safety, and public health. Therefore, it is necessary to strengthen applied research and consolidate integrated prevention and surveillance policies.Item Perfil leucocitario característico del leucograma de estrés en gatos(Universidad santiago de cali, 2025-11-04) Benavides Rodriguez, Carolina; Vargas Camargo, Oscar Julián (Director)The main objective of this project is to determine the characteristics of the stress leukogram in cats, considering that, currently, not only is there less documentation on this hematological phenomenon compared to other species such as dogs, but certain variations in the leukocyte profile associated with stress have also been identified, which makes it difficult to standardize parameters and diagnoses. Similarly, this research is based on the understanding that stress, rather than state of mind, is a psychological and physiological response of feline organisms to factors considered threats or changes in their normal environment (Buffington & Bain, 2021). Therefore, the project aims to categorize the main characteristics found in the stress leukogram in cats through a qualitative, descriptive, and cross-sectional documentary review, in which the latest articles and clinical cases published on the subject in the last semester of 2024 were reviewed. Thus, the main findings of this article revealed that, despite the existence of different stressors, the hematological response of the cats studied coincided in the three studies analyzed, demonstrating that lymphopenia, eosinopenia, and leukocytosis due to neutrophilia are key factors in characterizing stress leukogram in domestic cats.Item Beneficios de los probióticos en la salud intestinal porcina e implicaciones para la seguridad alimentaria humana(Universidad Santiago de Cali, 2025-11-04) Ospina Torres, Valeria; Cabrera Matajira, Carlos Emilio (Director); Guarín Patarroyo, Camilo Ernesto (Director)Se llevó a cabo una revisión sistemática de la literatura científica con el propósito de evaluar los beneficios de los probióticos en la salud intestinal porcina y sus implicaciones para la seguridad alimentaria humana. El proceso de investigación se llevó a cabo mediante una revisión bibliográfica en plataformas electrónicas como PubMed, Science Direct, Google Scholar, Scopus y Web of Science, durante 2020 y 2025, con un enfoque particular en artículos publicados en el idioma inglés. El presente estudio se centró exclusivamente en las publicaciones realizadas en revistas indexadas, con una clasificación Qualis A1-B2 o SCImago Q1-Q2. La estrategia de búsqueda se fundamentó en la combinación de descriptores relacionados con probióticos, microbiota intestinal, porcicultura y seguridad alimentaria. Se eliminaron los duplicados y la selección de datos se realizó siguiendo las directrices PRISMA (Proposal Registration of Systematic Review). La gestión bibliográfica se realizó mediante el uso del software Mendeley®. El análisis realizado incluyó un total de 15 artículos, siendo China el país con el mayor número de publicaciones. Los hallazgos confirman que el uso de probióticos representa una alternativa eficaz y sostenible al uso de antibióticos en la producción porcina. Los beneficios se manifiestan en tres dimensiones principales: (i) se observa una mejora en la salud intestinal, el bienestar animal y los parámetros productivos, (ii) se evidencia una disminución en la prevalencia de infecciones gastrointestinales y la disbiosis, y (iii) se contribuye a la seguridad alimentaria humana al reducir la carga de patógenos zoonóticos y los residuos antimicrobianos en la carne. A pesar de la ausencia de consenso en cuanto a la selección de cepas óptimas y la estandarización de dosis, la evidencia disponible respalda la integración de probióticos en la porcicultura moderna como un componente esencial para la sostenibilidad productiva y la inocuidad alimentaria.Item Relación entre la disbiosis intestinal y el desarrollo de las alergias alimentarias en perros, revisión sistemática(Universidad Santiago de Cali, 2025-11-04) Rosales Jiménez, Ligia Nicole; Correa Toro, Nelson William (Director)The canine gut microbiome is a complex ecosystem that plays essential roles in digestion, metabolism, and immune regulation. Under eubiosis, the main bacterial phyla contribute to homeostasis by producing short-chain fatty acids (SCFAs), which strengthen the epithelial barrier and promote the induction of regulatory T lymphocytes. However, factors such as antibiotic use, ultra-processed diets, and aging favor dysbiosis, characterized by a loss of microbial diversity, altered proportions of key taxa, and an increase in pro-inflammatory bacteria. These changes are associated with the development of food allergies, mediated by exacerbated Th2 responses and inadequate IgA production. Recent studies show that diet, mode of delivery, and early microbial exposure significantly influence immune system maturation, supporting the hygiene hypothesis. Targeted therapies, such as fecal microbiota transplantation, probiotics, and postbiotics, aim to restore the microbiota and induce immune tolerance. Understanding the interaction between microbiota and immunity allows for the development of preventative and therapeutic strategies to improve intestinal health and well-being in dogs.Item Precisión diagnostica comparativa de la citología, histopatología e inmunofluorescencia directa en el diagnóstico del pénfigo canino(Universidad Santiago de Cali, 2025-11-04) Fernández Aramburo, William Andrés; Guarín Patarroyo, Camilo (Director)This systematic review evaluated the comparative diagnostic accuracy of cytology, histopathology, and direct immunofluorescence in canine pemphigus through the analysis of 62 studies identified in five databases following PRISMA guidelines. The results demonstrated that each technique presents specific advantages and limitations: cytology showed high specificity but variable sensitivity depending on the lesion stage; histopathology confirmed its central role, although its effectiveness is compromised by sample selection; while direct immunofluorescence emerged as the most reliable method for confirmation, albeit with significant practical limitations. Therefore, it can be concluded that optimal diagnosis requires the integration of these techniques, considering their constraints and the specific clinical context. Thus, highlighting the need to establish more robust diagnostic guidelines for these techniques.Item Precisión diagnóstica y la utilidad clínica de los métodos microscópicos, moleculares (PCR) y serológicos en el diagnóstico de la coccidiosis en perros y gatos. Revisión sistemática(Universidad Santiago de Cali, 2025-11-04) Hernández Bravo, Bryan; Guarín Patarroyo, Camilo Ernesto (Director)Coccidiosis is a common intestinal parasitic disease in dogs and cats, mainly caused by protozoa of the Cystoisospora genus. Its diagnosis poses a clinical challenge due to the intermittent excretion of oocysts and the morphological similarity among species. The use of varied diagnostic methods—microscopic, molecular, and serological—aims to increase accuracy and optimize therapeutic decision-making in veterinary medicine. Objective: To evaluate the diagnostic accuracy and clinical utility of microscopic, molecular (PCR), and serological methods used in diagnosing coccidiosis in dogs and cats through a systematic review of recent scientific literature. Methodology: A descriptive and analytical systematic review of empirical studies published between 2020 and 2024 was conducted, using the databases PubMed, EBSCO, ScienceDirect, and Embase. The MeSH descriptors “Coccidiosis,” “Clinical Laboratory Test,” “Cats,” and “Dogs” were applied. Seventy-three articles were identified, of which twenty-eight met the inclusion criteria, and twenty-one were analyzed in depth. Results and Discussion: Fecal flotation microscopy remains the most widely used method due to its low cost and speed; however, its sensitivity barely reaches 60% in mild infections. Molecular techniques, such as PCR and qPCR, demonstrated sensitivities above 90%, allowing detection of subclinical infections and differentiation between species. Nevertheless, their routine use is limited by costs and technical requirements. Serological and coproantigen tests (ELISA, IFAT) provide intermediate tools with high sensitivity and are particularly useful in population studies or breeding facilities. Combining diagnostic methods significantly improves accuracy and clinical decision-making. Conclusions: No single method is ideal. The most effective diagnostic approach combines fecal flotation as an initial test, ELISA for early detection, and PCR for specific confirmation. Integrating these techniques optimizes sensitivity, specificity, and clinical utility in diagnosing coccidiosis in small animals.Item Parásitos gastrointestinales en crías de Zarigüeya común () en un hogar de paso de Santiago de Cali(Universidad Santiago de Cali, 2025-11-22) Molina Diaz, Maria Margarita; Castillo Aguirre, Esteban; Bermeo Sierra, Marta Liliana (Directora)Wildlife supports key ecological processes; the common opossum Didelphis marsupialis participates in seed dispersal and biological control. The purpose of this study is to fill a gap in the national literature on parasitism in newborns of this species and generate useful information for diagnostic, treatment, and management protocols that improve care in rehabilitation centers and prevent zoonotic hazards. The presence of gastrointestinal endoparasites in offspring cared for at the DAGMA foster home in Santiago de Cali and its relationship with the clinical status of admission were characterized. A 3-month descriptive and analytical study was conducted on 30 orphaned offspring weighing 50– 100 g, with standardized registration, stool collection by stimulation, and diagnosis by Sheather flotation with readings at 100×/400×; the analysis was performed in Excel. The results showed 26.7% positivity for protozoa, with a predominance of Trichomonas spp. (20.0%) and detection of Giardia sp. (6.7%; cysts and trophozoites), the majority asymptomatic, with no helminth eggs, and outcomes of 63.3% released and 36.7% dead, with a higher incidence in the urban south. The discussion interpreted a mostly subclinical parasitosis, conditioned by age and pre-patent windows, and emphasized that sucrose flotation is useful for screening, but limited for larvae and certain cysts. Therefore, it is proposed to complement it with specific techniques and serial samples, in addition to consolidating the shelter as a sentinel node for public health and conservation. The conclusions emphasize individualizing therapies based on signs and burden, avoiding mass treatments, and strengthening documentary standardization and the coprodiagnostic algorithm to improve traceability, efficacy, and community education, with declared scientific, social, and environmental impacts.