Microbiología

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Verificación de la técnica del método horizontal para la detección y el recuento de listeria monocytogenes en un laboratorio bioindustrial
(Universidad Santiago de Cali, 2024) Corrales Correa, Valeria; Baloco Sanchez, Luis Alexander; Restrepo Aura Falco; María Ximena, Figueroa
Listeria monocytogenes is a foodborne pathogen that causes listeriosis, which makes it a public health problem due to its clinical manifestations such as meningitis, septicemia, abortion, among others. The lack of quality controls in the food production process increases the risk of infection, so a quick detection is essential. In this work, the verification of the horizontal method for the detection and enumeration of L. monocytogenes in the SYNLAB Laboratory was carried out following the ISO 11290 Standard. For this purpose, the strains were standardized, the culture media were tested for productivity and selectivity and the horizontal method was performed. Good precision and accuracy were obtained by achieving reproducibility percentages higher than 73.7% and a value close to 90% in the concordance correlation coefficient, and, finally, a sensitivity and specificity of 100%. No significant figures were observed between the two analysts, demonstrating the effectiveness of the verification