Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
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Date
2015-09-01
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Publisher
Universidad Santiago de Cali
Abstract
An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation containing 0.5% hypericin was developed and assayed in vivo in a hamster model of cutaneous leishmaniasis. Results demonstrate that hypericin induces a significant antiamastigote effect in vitro against L. panamensis by decreasing the number of parasites inside infected cells. The topical formulation of 0.5% hypericin allows healing of L. panamensis-induced lesions upon a topical application of 40 mg/day plus visible-light irradiation (5 J/cm 2 , 15 min), twice a week for 3 weeks. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
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Keywords
Animals, Cell Line, Cricetinae, Humans, Leishmania, Leishmaniasis, Cutaneous, Perylene, Photochemotherapy, Photosensitizing Agents, U937 Cells, Wound Healing, drug absorption, drug efficacy, cytotoxicity, amphotericin B deoxycholate, epidermal growth factor, glyceraldehyde 3 phosphate dehydrogenase