Microbiología

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Comparación de dos técnicas para la captación de esporas fúngicas ambientales en un campus universitario
(Universidad Santiago de Cali, 2019) Delgado Ñañez, Cristhian; Caicedo Bejarano, Luz Dary
The concordance of two fungal spore uptake techniques in the environments of three buildings of the Santiago de Cali University campus was evaluated. The gravimetric method of spore uptake was compared with the Omeliansky formula and the volumetric method (Air Ideal 3P). The environmental sampling was carried out quarterly for a period of 12 months using Dicloran Rose Bengal Chloramphenicol agar (DRBC). 64497 CFU / m3 were accounted for, of which 34930 CFU / m3 were isolated with the volumetric method and 29567 CFU / m3 with the gravimetric method. No significant statistical differences were observed with the two techniques used (p = 0.0739), with a significance level α = 0.05. The predominant genera obtained with both methods were Cladosporium, Penicillium, Aspergillus and Fusarium, which suggests that although the gravimetric method is not as efficient as the volumetric method, it is reliable for estimating the degree of environmental fungal contamination in a similar way to the volumetric method.