Microbiología
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Browsing Microbiología by Subject "ACPM"
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Item Bacterias potencialmente degradadoras de hidrocarburos aisladas en Santiago de Cali, Valle del Cauca(Universidad Santiago de Cali, 2020) Machacado Salas, Maryuris; Rache Arce, Diana Carolina; Rosero García, Doris AmandaOil hydrocarbon pollution is one of the main problems around the world since it generates negative ecological, economic, and social impacts. Colombia has been affected by hydrocarbon spills that contaminate different environments such as water and soil. Bacteria have been reported with the ability to degrade hydrocarbons, becoming an excellent strategy for designing studies that contribute solutions to this problem. In the present study, a methodology was standardized to isolate hydrocarbon degrading bacteria in six biological oxidation lagoons and a pure leachate from a treatment plant located in the Old Navarro Landfill at Santiago de Cali city. Three tests were conducted: leachate contamination, disk test and tube test, and 17 bacterial morphotypes with degrading potential of gasoline, diesel and ACPM were found. The bacteria identified were Serratia marcescens (morphotype 97), Bacillus sp. (morphotypes 90, 117 and 263), Bacillus cereus (morphotype 120) and Exiguobacterium sp. (morphotypes 123 and 137). This work carried out to standardize a methodology to isolate bacterial morphotypes in the Old Navarro Landfill and allowed future studies to study the degradation mechanisms in bacteria, and solutions that contribute to the reduction of hydrocarbon contamination.