Browsing by Author "Caicedo Bejarano, Luz Dary (Directora)"
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Item Actividad antifúngica de Alpinia zerumbet frente a mohos y levaduras de importancia clínica(Universidad Santiago de Cali, 2020) Cruz Alemán, Lady Stephanie; Benavides Gálvez, Laura Isabel; Caicedo Bejarano, Luz Dary (Directora); Castro Narváez, Sandra Patricia (Directora)Invasive fungal diseases (EFI) diseases to susceptible hosts, cause high impact morbidity and mortality, associated with the high resistance to antimycotics of the agents etiological associated with this pathology. The antifungal activity of extracts of Alpinia zerumbet, vs. C. albicans, C. parapsilosis, C. krusei, Aspergillus fumigatus, A. terreus and Fusarium oxysporum was determined. The minimum inhibitory concentration (MIC) method and minimum inhibitory fungal concentration (CFI) based on NTC 2455 was used. As control sample, Fluconazole standard is considered and 96% Ethanol as a control. The alcoholic and hydroalcoholic extracts of the leaves and flowers affected good fungistatic and fungicidal activity when compared to the control and standard sample. It is concluded that the leaves compounds and flowers of A. zerumbet are promising for future research looking for compounds with antimicrobial activity for use in agriculture, food, and treatment of systemic mycosis.Item Biota Fúngica en fosas nasales de trabajadores de tres edificios de la Universidad Santiago de Cali y su relación con síntomas de alergias respiratorias(Universidad Santiago de Cali, 2019) Castillo Téllez, Beatriz Angélica; Caicedo Bejarano, Luz Dary (Directora)Allergenic fungi enter through the air to nostrils and may be responsible for some respiratory allergies. The objective of the present study was to determine the relationship between allergies diagnosed and undiagnosed according to the results obtained from the adapted survey of ISAAC (International Study of Asthma and Allergies in Childhood) and the fungal load of the nostrils of workers of three buildings of the Santiago de Cali University. The samples taken from the nostrils were made with sterile swabs and the subsequent sowing was done on Sabouraud dextrose agar, sunflower seed agar and Candida CHROMagar. In 100% of the workers (126) who participated in the study, allergenic fungi were isolated. The highest prevalence of allergenic fungi in the workers of the different buildings was obtained by the workers of block 1, finding significant statistical differences, for the genus Fusarium sp, (p=0,0018 Kruskal Wallis) and Penicillum sp, (p=0,0080 Kruskal Wallis), but not for Cladosporium sp, Alternaria sp and Aspergillus sp; likewise, the biggest fungal load was presented by the workers of this building. When analyzing the fungal 6 load found in the nasal passages and its relation with the presence of symptoms of respiratory allergies, it was established that there was significant statistical association with the genus Fusarium sp (p=0,0430 Wilcoxon Mann Whitney) and not for Cladosporium sp (p=0,1918, Wilcoxon Mann Whitney) which was the most frequently isolated genus.Item Caracterización fisicoquímica, sensorial y micológica del Kéfir de agua en jugos de frutas tropicales(Universidad Santiago de Cali, 2019) Pedraza Morales, Yiham Stephanie; Ramírez Navas, Juan Sebastián (Director); Caicedo Bejarano, Luz Dary (Directora)Kefir contains beneficial properties that promote health, which is why microorganisms isolated from kefir are studied for the development of functional foods. In the present study, the physicochemical, sensory and growth characteristics and phenotypic characterization of fungi present in water kefir in tropical fruit juices were determined. This experiment was done under a factorial design corresponding to the combination of two factors with three blocks and three replicas: type of substrate (pear juice, passion fruit and water with panela) and sucrose concentration (25%, 50% and 100%). The response variables were: acidity, pH, Brix degrees. For the microbiological analyzes, decimal dilutions were made, they were counted on the second and fifth day of incubation. The identification was made with API 20C and Vitek 2 (Biomérieux). Three yeast strains found from higher to lower number of CFU/mL Saccharomyces cerevisiae 1, Saccharomyces cerevisiae 2 and Zygosaccharomyces spp were finally isolated with the sensory analysis it was determined that the passion fruit drink was the one that had the best acceptance and preference on the part of consumers.Item Comparación de diferentes medios de cultivo para el aislamiento de hongos alergénicos a partir de ambientes y fosas nasales(Universidad Santiago de Cali, 2019) Concha Alarcón, Héctor Raúl; Caicedo Bejarano, Luz Dary (Directora); Chávez Vivas, Mónica (Directora)Different culture media were evaluated for the collection of environmental samples (Agar Dicloran Rosa de Bengala Cloranfenicol, CHROMagar Candida, Avena Agar and , Sabouraud Dextrosa Agar + 5% NaCl) and nasal fossa samples (Sunflower seed agar, SDA and CHROMagar Candida) of workers from three University buildings Santiago from cali. It was found that there were statistical differences, p>0.05, when CHROMagar Candida and Agar Dicloran Rosa de Bengala Cloranfenicol were compared with the volumetric method. , Sabouraud Dextrosa Agar + 5% NaCl recovered the lowest number of UFC / m3, p <0.0001 when compared with the other media used with the gravimetric method and in the study of nostrils it was found that SDA was the culture medium where the higher counts P<0.0001. In this study, the Agar Dicloran Rosa de Bengala Cloranfenicol culture medium demonstrated that it is the medium of choice for the isolation of fungi from environmental samples and Sabouraud agar for clinical samples.Item Comparación de dos técnicas para la captación de esporas fúngicas ambientales en un campus universitario(Universidad Santiago de Cali, 2019) Delgado Ñañez, Cristhian; Caicedo Bejarano, Luz Dary (Directora)The concordance of two fungal spore uptake techniques in the environments of three buildings of the Santiago de Cali University campus was evaluated. The gravimetric method of spore uptake was compared with the Omeliansky formula and the volumetric method (Air Ideal 3P). The environmental sampling was carried out quarterly for a period of 12 months using Dicloran Rose Bengal Chloramphenicol agar (DRBC). 64497 CFU / m3 were accounted for, of which 34930 CFU / m3 were isolated with the volumetric method and 29567 CFU / m3 with the gravimetric method. No significant statistical differences were observed with the two techniques used (p = 0.0739), with a significance level α = 0.05. The predominant genera obtained with both methods were Cladosporium, Penicillium, Aspergillus and Fusarium, which suggests that although the gravimetric method is not as efficient as the volumetric method, it is reliable for estimating the degree of environmental fungal contamination in a similar way to the volumetric method.Item Dermatofitos en suelos del zoológico de la ciudad de Cali, Colombia(Universidad Santiago de Cali, 2022) Quintero Barragán, Daniel; Grisales Toro, Anyelly; Caicedo Bejarano, Luz Dary (Directora); Ramírez Castrillón, Mauricio (Director)The dermatophyte fungi present in the soils of the animal houses of the Zoological of the city of Cali, Colombia, were studied. The objective of the study was to determine the presence of dermatophyte fungi in these habitats and the variation of their presence according to some physicochemical parameters of the soil. The Vanbreuseghem hair-hook technique was used, and measurements of pH, conductivity, organic matter, ash, and moisture percentage were also taken. 37/60 (61.6%) of the samples were positive for the Nannizzia gypsea complex; no other dermatophyte was isolated. Soil pH values ranged from 4.63 to 9.36 and the isolation rate of the fungus was higher in soils with a pH range of 5.5 to 6.9 and this difference was statistically significant. The other physicochemical parameters were not significant for the presence of the fungus.Item Estructura de comunidades bacterianas con resistencia a los antibióticos en los sistemas de aguas (Una revisión narrativa, aportes para la vigilancia epidemiológica)(Universidad Santiago de Cali, 2021) Sánchez Baena, Ana María; Caicedo Bejarano, Luz Dary (Directora); Chávez Vivas, Mónica (Directora)Item Estudio de la Micota Ambiental de tres edificios de la Universidad Santiago de Cali, Colombia(Universidad Santiago de Cali, 2019) Lasso Ceballos, Ana María; Caicedo Bejarano, Luz Dary (Directora)The quantification of the environmental load in the indoors of the buildings (1, 3 and 4) of the University Santiago De Cali, Colombia and in their corresponding responses to the results to obtain a load value. A volumetric method and Dicloran Bengal chloramphenicol (DRBC) and CHROMagar Cándida culture media were used for sampling. The identification of the fungi was carried out based on macro and microscopic characteristics. Parallel to the sampling the relative humidity and temperature of each space studied was determined. This study was conducted for quarterly periods during twelve months (March 2018 to march 2019). In the study of environments of the three buildings was detected a total of 82393 colony forming units (CFU/m3) of which 60053 CFU/m3correspond to indoor environment. The results obtained showed that it was not possible to establish a correlation between the temperature and the CFU/m3 found inside the three buildings, but for the relative humidity in relation to building 4.The genera Cladosporium, Fusarium, Penicillium and Aspergillus were identified as the most prevalent allergenic fungi in this study. The results in the quantification revealed that the fungal burden exceeds the limits accepted for internal environments (500 CFU/m3) according to the World Health Organization (WHO).Item Estudios de Chlorella spp en Colombia. Revisión sistemática: Métodos de cultivo y sus aplicaciones(Universidad Santiago de Cali, 2022) Banguera Obando, Julián; Varela Santiago, Daniel; Caicedo Bejarano, Luz Dary (Directora); Ortiz Monsalve, Santiago (Director)The world's population is constantly increasing, and it is expected that by the year 2050 there will be approximately 9 billion people. Industrialization, pollution, consumption of food, energy, and greenhouse gases, is a problem that must be faced with the commitment to reduce carbon dioxide by 50% to 80%, which would allow preserving climate and food security. In Colombia, the cultivation of microalgae has already been implemented in several industrial sectors, agribusiness, the agricultural sector and in the environmental area. The species of the Chlorella genus are among the most used in the country, to supply consumption needs and for wastewater treatment, due to their capacity to bioaccumulate and biodegrade heavy metals, herbicides, phenols, among other compounds, which is why they are used in phycoremediation processes. It has also been used in research based on the fixation of atmospheric carbon dioxide with positive results. To know the biotechnological use of microalgae in Colombia, a systematic review of the species of Chlorella spp. was carried out to know the cultivation conditions and applications of these microalgae on a national scale. The alternative use of Chlorella spp. is effective compared to other conventional processes used in multiple applications and good results are obtained under correct cultivation conditions. The information obtained in the selected articles offers some bases for the continuity and improvement in applications such as phycoremediation, protein production, biofuels, pigments, among others. The most used Chlorella species in Colombia, such as C. vulgaris and C. sorokiniana, and the multiple cultivation conditions are also presented, establishing that there is no standardization in these processes that would allow achieving the maximum use on an industrial scale of this promising group of microalgae.Item Hongos saprófitos potencialmente patógenos en ambientes internos del Zoológico de Cali, Colombia(Universidad Santiago de Cali, 2025) Sánchez Guerra, Anny Marcela; Domínguez Pérez, Daniela; Caicedo Bejarano, Luz Dary (Directora); Martínez Garay, Carlos Andrés (Director)The study describes for the first time the fungal load of indoor and outdoor environments of the animals of the Zoo of the city of Cali, Colombia. Bimonthly samples were taken for 4 months in 14 animal habitats and 5 in outdoor (visitor) environments, simultaneously recording the temperature and relative humidity of the environment. For sampling, the horizontal gravitational deposition method was applied with the Omeliansky formula and the culture media used were DRBC Agar, Chromagar Candida, and SDA Agar. Pathogenicity tests were performed on the isolated yeasts. The analyses showed no significant differences in the number of CFUs/m3 of air between the indoors and outdoors, but there are differences between the sampled interiors. The results indicate that the fungal load exceeds 1000 CFU/m³ in several areas, posing a risk to public health. High fungal diversity was observed. The most frequently isolated fungi were yeasts and species of Cladosporium, Penicillium, Aspergillus, Fusarium. The presence of Aspergillus fumigatus was determined in 5 of the interiors studied. It is concluded that the conditions of the Cali Zoo, due to its abundant vegetation and presence of organic matter, may favor the proliferation of fungi with pathogenic potential. Monitoring is recommended at least twice yearly to avoid health risks to animals and those caring for them.Item Revisión sistemática: Presencia de Clamidosporas en algunos de los aislamientos del Género Ganoderma (Ganodermataceae)(Universidad Santiago de Cali, 2023) Balanta Mejía, María Camila; Caicedo Bejarano, Luz Dary (Directora)A qualitative systematic review of scientific articles was carried out to verify the presence or not of chlamydospores as a taxonomic character and their morphological characteristics, as a contribution to the differentiation of species of the genus Ganoderma. For the systematic review, Scopus, Science Direct, Taylor & Francys, and Springer databases were searched for articles published between 1965 and 2022. Some elements of the PRISMA (Preferred reporting item for systematic reviews and meta-analyses) methodology were used for this study. This review allowed us to know which species of the genus Ganoderma have as taxonomic character the presence or not of chlamydospores, however, the establishment of taxonomic identities through the presence of these is complex, due to the plasticity of the species and the lack of a consensus in the letters, numbers and terminology used, so it is necessary to carry out new studies to identify the interspecific and intraspecific variability to know the taxonomic plurality of the genus.Item Sensibilidad cutánea (prick test) a hongos alergénicos de los trabajadores de tres edificios de la Universidad Santiago de Cali y su relación con los síntomas de alergias respiratorias(Universidad Santiago de Cali, 2019) Calderón Quintero, Cristina; Caicedo Bejarano, Luz Dary (Directora); Serrano Reyes, Carlos Daniel (Director)An observational epidemiological cross-sectional study was proposed, a relation to the exposure of Fungi in intramural environments and the presence of allergenic fungi in nasal fossae was described. The study involved 126 workers from the Santiago de Cali University, who signed an informed consent and answered the adapted ISAAC survey (The International Study of Asthma and Allergies in Childhood) to determine the prevalence of diagnosed and undiagnosed allergies. 71/126 (56.3%) worked in block 1; 24/126 (19.1%) in block 3 and 31/126 (24.6%) in block 4, The "in vivo" Skin Prick Test (SPT) was performed on 75 workers. A prevalence of SPT + was found for Fusarium oxysporum of 10/75 (13.3%), Aspergillus fumigatus 5/75 (6.7%), Penicillium notatum 5/75 (6.7%), Alternaria alternata 5/75 ( 6.7%) and Cladosporium herbarum 1/75 (1.3%). This study allowed to establish a possible association between the presence of fungi in the labor environment and in the nostrils of workers with the positive response for the SPT test for Fusarium oxysporum.Item Susceptibilidad a los antimicóticos de levaduras aisladas de los sistemas de aguas de la ciudad de Santiago de Cali, Colombia(Universidad Santiago de Cali, 2019) Osorio Vanegas, Lizeth Stefania; Caicedo Bejarano, Luz Dary (Directora); Ramírez Castrillón, Mauricio (Director)Fungal infections increased markedly on the world. Currently, new pathogenic yeast species are reported with more frequence. The most important sources of aquatic isolation are industrial and municipal wastewaters, represented by chemical, physical and biological pollutants. The aim of this study was to characterize the antifungal susceptibility of yeasts isolated from, water systems in Cali, Colombia. Also to assens the correlation between physicochemical parameters, heavy metals, and fluconazole susceptibility, one of the most used antifungal. Two types of water were collected: potable (Río Meléndez, Puerto Mallarino) and residual wastewaters (Canal Sur, Planta de Tratamiento Cañaveralejo - PTAR). Serial dilutions were made and the samples were cultured on Dicloran Rose Bengal Chloramphenicol (DRBC) agar (Merck) and CHRomagarCandida (BBL) to count the CFU/mL. Study of susceptibility to Amphotericin and Fluconazole were made by the plate microdilution technique. Groupings were carried out according to their morphology and the identification of the representative isolates of each morphotype was carried out by biochemical kit (API 20C) and analysis of sequences of the ITS1-5.8S-IST2 region. 15 yeast species were identified with API 20C and six species by molecular biology corresponding to: Candida albicans, Pichia fermentans, Diutina catenulata, Rhodotorula mucilaginosa, Trichosporon coremiiforme and Pichia kluyveri. It was reported two morphotypes corresponding to new species of Candida.