Microbiología
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Browsing Microbiología by Author "Candelo Montaño, Javier"
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Item Estudio del crecimiento de Pseudomonas fluorescens en sustratos biopolímericos sintetizados a partir de residuos agroindustriales de Manihot esculenta(Universidad Santiago de Cali, 2024) Candelo Montaño, Javier; Palencia Luna, Sixta; Garcés Villegas, VivianaCurrently, plastic pollution from single-use plastics in water and soil is on the rise, causing damage to the ecosystem. Therefore, the development of new technologies for the manufacture of more environmentally friendly materials is a promising alternative to mitigate this damage. The manufacture of bioplastics from organic materials has enormous potential due to the use of residual biomass from various agro-industrial processes. Therefore, the objective of this research was to evaluate the growth of Pseudomonas fluorescens ATCC13525 on two bioplastics synthesized from starch and residues from the cultivation of Manihot esculenta, specifically leaves and stems. The strain was isolated and morphologically studied, and growth was evaluated from the exposure of the substrates to two different culture media. The results determined that the strain had an ascending growth with an average cell density of 18.6 x 108 CFU/ml at 48 hours. Likewise, the percentage of mass loss was higher in the bioplastics with 50%. Finally, optical microscopy at 10x confirmed changes in all samples with the appearance of opacity, which translates to the presence of an attached biofilm, which is associated with microbial activity. These biofilms could have excellent application since P. fluorescens, being a rhizobacterium, could metabolize biomass and at the same time synthesize plant growth-promoting substances, therefore, the negative impact of the bioplastic on the environment would be mitigated. The evaluation through FTIR allowed to observe changes along the spectrum and in the range of 1800 to 1300 cm-1 where signals corresponding to the ⱱd C=O and N-H bonds and ⱱt N-H of peptidoglycan deposited as a biofilm in the different polymeric materials were identified