Microbiología

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Exploración de la selectividad de colistina hacía Escherichia coli y de daptomicina hacia Staphylococcus aureus mediante espectroscopia infrarroja con transformada de Fourier
(Universidad Santiago de Cali, 2023) Gómez Martínez, Andrés; Vélez Bueno, Isaac Eduardo; Oñate Garzón, José Fernando; Ausili, Alessio
Colistin and daptomycin are clinically important antimicrobial peptides (AMPs) which are bactericidal depending on whether it is a Gram-positive or Gram-negative bacterium; both peptides are characterized by altering the permeability and organization of the cell membrane, therefore, the evaluation of the interaction between both PAMs and E. coli 25922 and S. aureus 25923 cells was carried out, analyzing membrane functional groups by Fourier Transform Infrared Spectroscopy (FTIR). In this work we report band shifts and vibrations in methyl, methylene and phosphate functional groups of the cell membrane in both strains as a consequence of interaction with colistin, unlike daptomycin which only caused representative changes in S. aureus. This establishes FTIR as a tool to explore the activity and selectivity of various PAMs in strains to combat antimicrobial resistance