Microbiología

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Efecto del estrés celular en la producción de pigmentos en la cepa Rhodotorula mucilaginosa/alborubescens AJB01 aislada en el Caribe Colombiano
(Universidad Santiago de Cali, 2020) García Cortés, Alejandra; García Vásquez, Julián Andrés; Ramírez Castrillón, Mauricio (Director); Aranguren Díaz, Yani Cristina (Directora)
Most of the dyes or pigments used in the industry are toxic and potentially mutagenic, with some carcinogenic effects, so their replacement and disposal of industrial effluents is a priority task. Therefore, searching microbial bio-pigments is of great interest in the industry. The objective of this work was to obtain the overproduction of carotenoid pigments in a naturally pigmented yeast through the application of different sources of cellular stress. The strain AJB01 was isolated from a sample of food obtained in Barranquilla, Colombia and its main pigment is 𝛽-Carotene. This strain was subjected to different conditions, such as osmotic stress with different salts, physical stress from exposure to UV light and light stress from exposure to different photoperiods. The best culture conditions for obtaining carotenoids were: one minute of ultraviolet light, 0.5 mg/L of magnesium sulfate and 18:6 hours of photoperiod, respectively, generating a yield of 118,33 µg/g carotenoids.