Microbiología
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Browsing Microbiología by Author "Aranaga Arias, Carlos Andrés"
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Item Determinación de los cambios que genera la presencia de los Iones Mg2+, Ca2+, y Na+en la proliferación de salmonelófagos líticos(Universidad Santiago de Cali, 2024) Guazá Balanta, María José; Aranaga Arias, Carlos Andrés; Falco Restrepo, Aura DayanaOne of the most common foodborne diseases worldwide is salmonellosis, which is caused by the bacterial genus Salmonella spp. The resistance to antibiotics that this bacteria has conferred begins the search for alternatives for its treatment, phage therapy being one of these. The adhesion of bacteriophages to the cell surface is the first and one of the main stages in viral infection. There are ions such as Mg+2, Ca+2 and Na+ that work as cofactors for the adhesion of bacteriophages to their hosts, resulting in an increase in the viral population. That is why in this work, based on the determination of the viral titer of a bacteriophage infective for Salmonella (4x109 PFU/mL), the changes in its proliferation in the presence of Mg+2, Ca+2 and Na+and ions were determined. The Mg+2 ion was the one in which changes were found that can potentiate the obtaining of the bacteriophage from a complex sample such as a solid environmental matrixItem Evaluación de Antibióticos para el Control de Bacterias Ácido-Lácticas y Ácido Acéticas en el proceso de producción de alcohol carburante en el Valle del Cauca(Universidad Santiago Cali, 2020) Gomez Capote, Jennifer Lorena; Rojas Mora, Angélica María; Aranaga Arias, Carlos Andrés; Silva Aguas, SantiagoThe antimicrobial capacity of the bacteriocin nisin and the antibiotics neomycin and oxytetracycline was evaluated against the control of contamination caused by Lactic Acid Bacteria (LAB) and Acetic Acid Bacteria (AAB) in fermentative processes. For this, the Minimum Inhibitory Concentration (MIC) of each one was determined and fermentation tests were carried out on a laboratory scale at a concentration of 40 ppm. These tests showed that the evaluated molecules managed to decrease the contamination caused by LAB and AAB during the 48 hours of the test. During fermentation the cellular viability of Saccharomyces cerevisiae was maintained at over 98 percent, using oxytetracycline. For this reason they are proposed as a model for the control of bacterial contamination by LAB and AAB in fermentations.Item Evaluación de la actividad antimicrobiana de un desinfectante de extractos naturales, sobre el crecimiento de bacterias y hongos provenientes de ambientes industriales e institucionales(Universidad Santiago de Cali, 2020) Cañón Castro, Sofía; Palacio Muñoz, Nicolás; Aranaga Arias, Carlos AndrésCleaning and disinfection in hospital, industrial and institutional environments is an essential procedure for controlling the risk of disease transmission, as well as contaminating microorganisms. However, many chemical disinfectants pose a risk to the environment because they can form toxic compounds that are difficult to handle. The objective of this work was to determine the antimicrobial efficacy of a disinfectant, whose active ingredients are natural extracts, which minimizes the environmental impact. For this, this disinfectant was validated under Colombian Technical Standards 5150 and 5817. Additionally, its activity was verified on isolates of lactic acid and acid-acetic bacteria contaminating fermentation industries, as well as environmental yeasts of an educational institution. The results obtained show that the disinfectant is effective for the disinfection of bacteria and fungi found in institutional and industrial environments, being an alternative to the use of chemical disinfectantsItem Evaluación de la capacidad inhibitoria de extractos de curuba y moléculas bioactivas sobre el crecimiento de mycobacterium smegmatis mc2155.(Universidad Santiago de Cali, 2019) Anacona Sánchez, Paola Andrea; Aranaga Arias, Carlos AndrésThe intrinsic resistance that mycobacteria show to some disinfectants and antibiotics makes them a growing public health problem. Due to the fact that natural products and their derivatives have been shown to be a source of new antimicrobials, the effectiveness of hexanic, ethanolic and methanolic extracts of cascara and pulp of the fruit of the curuba (Passiflora mollisima) and some chalcone molecules synthesized by the Research Group of Heterocyclic Compounds of the Universidad del Valle (Colombia), on the growth of Mycobacterium smegmatis mc2155. The results showed that the hexanic extract of peel and a chalcone molecule, were able to inhibit the growing mycobacterium at concentrations of 1 mg/ml and 100 μM, respectively. This demonstrates the potential of curuba, to obtain molecules that, like the chalcones, serve as the basis for obtaining and designing new drugs.