Microbiología
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Browsing Microbiología by Author "Aranaga Arias, Carlos Andrés"
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Item Determinación de la actividad antifúngica de extractos vegetales de Piper aduncum y Thevetia peruviana(Universidad Santiago de Cali, 2022) Cossio Banguero, Alejandra; Aranaga Arias, Carlos AndrésThe yeast Candida albicans is an opportunistic pathogen capable of causing serious systemic infections in immunocompromised individuals. Several studies have demonstrated the antimicrobial properties of plant extracts, due to the ability of plants to synthesize a wide variety of bioactive compounds. In this research, the antifungal activity of dichloromethanolic, methanolic and ethyl acetate extracts of Piper aduncum and Thevetia peruviana plants on the growth of C. albicans strains ATCC 14053 and ATCC 10231 was evaluated by agar dilution and broth microdilution to determine the Minimum Inhibitory Concentration (MIC) and establish whether the extracts have fungistatic or fungicidal activity. It was evidenced that the dichloromethane extract of P. aduncum was the only one to present inhibitory activity against the strains at an extract concentration of 1000 µg/mL and presented MICs that were in a range between 256->1024 µg/mL, finally, it was established that the extract presented fungistatic activity as a result of presenting a MIC:MFC (Minimum Fungicidal Concentration) ratio higher than 4. Finally, the presence of medium polarity molecules in the P. aduncum plant with inhibitory activity against C. albicans was indirectly evidencedItem Evaluación del efecto inhibitorio de los pigmentos extraídos de Aureobasidium spp frente a Lactobacillus spp. aislados de la fermentación alcohólica(Universidad Santiago de Cali, 2022) Samboni Tenorio, Alejandro; Parrado, Darly Silvana; Aranaga Arias, Carlos AndrésMelanin is one of the most widely distributed pigments in nature and among other functions, it has been attributed to a great antibacterial activity. In the present study, the production of the melanin-type pigment was determined in crude extracts obtained from four environmental isolates of yeast-like fungi of the genus Aureobasdium (LC 112, YAM1, YAM2 and YAM4), as potential inhibitors of bacteria of the genus Lactobacillus contaminating the alcoholic fermentation and the yeast of Saccharomyces cerevisiae alcohol producer. For the production of the pigment, the Aureobasdium isolates were grown in an inducing medium (KH2PO4 0.1%, (NH4)2 SO4 0.05%, MgCl2-6H2O 0.05% and glucose 5%), with constant agitation for 120 hours. To obtain the extract, the cells were harvested, lysed and acetone was used as extraction solvent. Once the extracts were obtained, the Minimum Inhibitory Concentration (MIC) was determined by microdilution assay in broth with resazurin. The results show a higher extraction efficiency in the isolate YAM2 (2.1 g/L) followed by YAM1 (1.66 g/L), YAM4 (1.06 g/L) and LC112 (1.03 g/L).), while the MIC for all tested microorganisms was greater than 2 mg/ml. Since no extract showed antimicrobial activity at the maximum concentration established in this work, it is proposed to quantify the concentration of melanin present in each extract. In this way, a theoretical concentration could be established to carry out microbial inhibition tests that allow establishing the real potential of the use of Aureobasdium extracts in the inhibition of contaminating microorganisms in the alcoholic industry