Microbiología

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Caracterización molecular de cepas de Escherichia coli extraintestinal (ExPEC) resistentes a cefalosporinas de tercera generación aisladas de centros hospitalarios en la ciudad de Cusco-Perú
(Universidad Santiago de Cali, 2022) Escandón Bonilla, Laura Isabel; Muñoz Delgado, Juan Diego; Correa Bermúdez, Adriana María del Socorro; Aguilar Ancori, Elsa Gladys
Escherichia coli (E. coli) is part of the gastrointestinal microbiota and is the cause of gastrointestinal and extraintestinal infections (ExPEC). Virulence factors (VF) allow differentiation of ExPEC when they contain at least two of the following genes: papA/papC/sfa/foc/afa/draBC/kpsMII/iutA. In Peru, E. coli is among the 5 most important bacteria and has resistance to cephalosporins associated with the presence of extended-spectrum beta-lactamases (ESBL) around 30% of cases. According to the above, the objective of the study was to characterize E.coli resistant to third-generation cephalosporins isolates such as ExPEC. For this, 77 isolates were analyzed using CHROMID® ESBL, a synergy test was done to confirm ESBL and PCR to identify VF. It was found that 75/77 isolates were ESBL producers and the virulence profiles were associated with the iutA, fyuA, fimH, kpsMTII, ompT genes. Only 22 of the isolates could be characterized as ExPEC.