Química Farmacéutica
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Browsing Química Farmacéutica by Author "Castillo Ayala, Jorge Enrique (Director)"
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Item Determinación del contenido de compuestos fenólicos y actividad antioxidante del extracto etanol/acuoso de la pulpa de arazá (Eugenia stipitata)(Universidad Santiago de Cali, 2025) Peña Ardila, Leidy Carolina; Montenegro Mora, Estefanía; Castillo Ayala, Jorge Enrique (Director)The objective of this work is to determine the antioxidant capacity of the phenolic compounds present in the pulp of arazá (Eugenia spititata Mc Vaugh), for this the extraction of bioactive compounds was carried out in three different media (aqueous, hydroethanolic 50:50 , ethanolic) in which a yield percentage of 2.9%, 3.5%, 3.3% respectively was obtained. Next, a phytochemical test was carried out to qualitatively identify the presence of bioactive compounds present in the extracts. Subsequently, the content of phenolic compounds was quantified by the folin-Ciocalteu method for each of the extracts taking gallic acid as a reference standard, where it was observed that the ethanolic extract had a higher content of phenolic compounds with a value of 506 .2500 equivalents in mg/L of gallic acid (GAE) in the extract. Finally, the antioxidant capacity of each of the extracts was evaluated by the ABTS method, resulting in an inhibitory concentration of 50 (IC50) for the ethanolic extract of 10.01mg/L, the hydroethanolic extract of 16.65mg/L, and finally the aqueous of 19.11mg/L, these results were compared with the reference standard and the antioxidant capacity equivalent to Trolox was calculated.Item Validación del método analítico por cromatografía de gases para la cuantificación de acetaldehído en ácido acético natural usado en la industria farmacéutica(Universidad Santiago de Cali, 2025-10-09) Cañas Cabrera, Jeniffer Sofía; Castillo Ayala, Jorge Enrique (Director)A gas chromatography method with flame ionization detection (GC-FID) was developed and validated for the quantification of acetaldehyde as an impurity in natural acetic acid. The validation followed USP <1225>, AOAC (2016), and ISO/IEC 17025 guidelines, assessing parameters such as selectivity, limit of detection (LOD), limit of quantification (LOQ), linearity, accuracy, precision (repeatability and intermediate precision), and residual analysis. The method exhibited excellent linearity in the range of 0.0021 to 0.0069 % w/w, with a correlation coefficient of 0.9997. Recovery values ranged from 104.5% to 108.3%, meeting AOAC acceptance criteria for the concentration range studied. Residual analysis confirmed the statistical validity of the linear model. Precision showed coefficients of variation below 5%. In conclusion, the method is sensitive, accurate, precise, robust, and suitable for quality control of natural acetic acid.