Facultad de Ciencias Básicas
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Browsing Facultad de Ciencias Básicas by Subject "16S rRNA"
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Item Efectos de diferentes dietas en la microbiota intestinal en perros (Canis familiaris) en la ciudad de Cali(Universidad Santiago de Cali, 2025-12-04) Lugo Rojas, Sthefany; Duque Zapata, Juan Diego (Director); Cardona Tobar, Karen Melissa (Director)In recent years, feeding dogs raw diets such as the BARF (Biologically Appropriate Raw Food) model has gained considerable attention among pet owners. This growing interest is driven by the belief that a more natural diet may improve digestive and overall health. However, variations in composition and processing between these raw diets and commercial extruded foods can significantly alter the intestinal microbiota, whose stability is essential for digestion, immune function, and disease prevention. A metabarcoding approach based on 16S rRNA gene sequencing (V3–V4 region) was used to compare the composition of the intestinal microbiota in dogs fed commercial kibble and BARF diets based on meat and chicken. The findings indicated that BARF diets contained a higher abundance of proteolytic genera such as Fusobacterium and Bacteroides, which are associated with protein degradation and the production of short -chain fatty acids, including propionate and butyrate. In contrast, the extruded commercial diets exhibited a mixed-type microbiota, where fermentative genera such as Phocaeicola, related to complex carbohydrate metabolism, coexisted with proteolytic bacteria such as Bacteroides and Fusobacterium. Additionally, genera with important metabolic and immunological roles—such as Megamonas, Clostridium, and Faecalibacterium—were detected. The variat ion observed among diet groups demonstrates that both nutritional composition and food processing directly influence bacterial metabolic pathways and, consequently, the functional balance of the intestinal ecosystem.