Facultad de Ciencias Básicas
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Item Ensamblaje de Novo del genoma de un aislado de pseudomonas SP como bacteria promotora del crecimiento de las plantas (PGPB) basado en la tecnología de secuenciación Minion(Universidad Santiago de Cali, 2023) Perlaza Barragán, Julián Andrés; López Álvarez, Diana Carolina; Corrales Ducuara, Alba RocíoModern agriculture has forced an increase in the production of agricultural crops by providing the intensive use of chemical fertilizers that have had a devastating effect on soil quality. In addition, climate change has generated a negative impact on the quality of soils used for agriculture, thus putting food security at risk. PGPRs are promoted as a sustainable application that will reduce the use of chemical fertilizers and pesticides. Through nanopore sequencing of the MinION platform, the sequence and de novo assembly of the genome of a Pseudomonas protegens isolate from Galleria mellonella larvae found in plantain soils was obtained. The chromosome had a size of 7,055,130 (bp) with a total of 6399 protein-coding genes. On the other hand, a total of 9 genes involved in promoting plant growth were identified, of which genes that encode phytohormones, phosphorus solubilizers and denitrifying enzymes were present. In addition, 25 genes involved in the control and suppression of phytopathogens were found, being mostly siderophores, antibiotics and biocontrol factors. The results showed that the isolated Pseudomonas protegens is a possible candidate as a biocontrol agent for use in crops of agricultural interest