Browsing by Author "Caicedo Bejarano, Luz Dary"
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Item Características fisicoquímicas y actividad antimicótica del extracto de tomillo sobre cepas Fusarium oxysporum(Universidad Santiago de cali, 2013-08-26) Balanta, Juan Fernando; Ramirez, Leo; Caicedo Bejarano, Luz DarySe determinó la composición química y la actividad antimicótica del aceite esencial y extracto alcohólico de las hojas y tallos del tomillo (Thymus vulgaris) planta de la familia Lamiaceae, por medio del método de Concentración Mínima Inhibitoria [CMI], NoItem Degradación del polietileno de baja densidad mediada por hongos filamentosos: Una revisión sistemática(Universidad Santiago de Cali, 2023) Banguera Ortiz , Adriana Sofia; Rangel Hoyos, Patricia; Rojas Barreto, Julián Alonso; Caicedo Bejarano, Luz DaryLow-density polyethylene (LDPE), is used in the food, clothing, transportation, construction, and medical industries. It is a high-consumption material and due to its physicochemical nature is extremely durable, recalcitrant, relatively unbreakable, and could be present for more than 50 years in the environment. Their accumulation generates serious pollution damage affecting natural resources, threatening fauna, wildlife, and even public health. In most landfills plastic waste represents the largest fraction, reaching up to 25% by weight in some sites. Degradation of LDPE can occur by chemical, physical, and biological methods, which can be affected by temperature, UV light, and enzymatic actions of both bacterial and fungal microorganisms. In biodegradation studies, fungi are considered potential candidates for LDPE degradation, due to their ability to adhere to the polymer surface and the production of intra- and extracellular enzymes. This review reports fungi isolated from LDPE-rich landfills with LDPE biodegradative capacity. Some fungi reported were of the genus Aspergillus, Penicillium, Rhizopus, among others. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) techniques are used to measure biodegradation and although other reviews have been analyzed, there is little information on fungi extracted from landfills used for LDPE biodegradationItem Etiopatogenía de la Adiaspiromicosis(Universidad Santiago de cali, 2012-09-30) Caicedo Bejarano, Luz DaryLa adiaspiromicosis es una enfermedad pulmonar que se conoce desde 1942, está asociada a pequeños mamíferos de las familias Rodentia, Carnívora y Mustelidae y rara vez se presenta; desde 1964 se han reportado aproximadamente setenta casos, principalmente en Brasil. El agente etiológico es Emmonsia crescens, un hongo geofílico, que se adquiere por inhalación de las conidias, el cual aumenta considerablemente su tamaño y se convierte en adiaspora en el tejido pulmonar o “in vitro” a 37°C; son estructuras de paredes muy gruesas, que no se multiplican ni diseminan e inducen la formación de granulomas epiteliodes rodeadas de una reacción eosinofílica. El diagnóstico se realiza por histopatología y cultivos y el tratamiento no es aún claro.Item Evaluación de procesos fotoquímicos de oxidación avanzada para la inactivación y/o reactivación de una cepa de Candida albicans(Universidad Santiago de Cali, 2024) Zamora Adrada, Emerson Yesid; Guerrero Rivera, Daniela; Caicedo Bejarano, Luz Dary; Castro Narváez, Sandra PatriciaCandida albicans is a priority fungal pathogen for the OMS, opportunistic, causing diseases in skin, mucous membranes, and invasive fungal infections with very high mortality rates. This study determined the effect of some advanced oxidation processes on the inactivation and reactivation rate of a C. albicans strain. For this, 9 inactivation assays (PDS, PMS, H2O2, UVC, PDS/UVC, PMS/UVC, H2O2/UVC, Photo-Fenton pH 3.4, Photo-Fenton pH 7.29) and 4 reactivation assays (UVC, PDS/UVC, PMS/UVC, H2O2/UVC) were performed. The best results were obtained with the UVC/PMS and UVC/H2O2 combinations with 100% removal at 30 seconds (s), followed by UVC/PDS from 45s and UVC light at 60s. The oxidants PMS achieved 98% removal at 60s; PDS 89% at 80s; H2O2 60% at 80s. The Photo-Fenton process showed a maximum removal of 41% and 50% at pH 3.4 and 7.29, respectively, at 60s. Regarding the reactivation of the C. albicans strain, it was determined that the minimum exposure time for the inhibition of C. albicans without reactivation was 45s for PMS/UVC and 60s for UVC, PDS/UVC, and H2O2/UVC. In conclusion, these advanced oxidation processes were effective methods for the inhibition of a C. albicans strain, with the PMS/UVC assays being the most efficient, followed by H2O2/UVC, PDS/UVC, UVC light, PMS, PDS, and finally, H2O2Item Evaluación de propiedades físicas, químicas y biológicas de un suelo después de ser usado como sustrato en humedales artificiales(Universidad Santiago de cali, 2015-09-20) Lugo Cardona, Angélica María; Plaza Alzate, Laura Catalina; Cisneros Rojas, Carlos A.; Caicedo Bejarano, Luz DaryEl suelo a pesar de ser indispensable para el desarrollo de la vida es un recurso no renovable por esta razón es importante el análisis de sus propiedades para determinar su fertilidad. Se evaluaron algunas propiedades físicas, químicas y biológicas de un suelo comercial usado como sustrato en humedales artificiales de flujo subsuperficial para la biorremediación de aguas residuales domésticas, estimando la intervención del Penicillium sp. y la planta Stipa ichu. Los resultados se sometieron a análisis de varianza (ANOVA) y prueba Tukey con una significación del 95%. Se encontró que el tratamiento con Stipa ichu presentó mayor remoción de N y proliferación de hongos solubilizadores de fosfato (HSF). Por otra parte, la Stipa ichu y el Penicillium sp favorecieron la capacidad de intercambio catiónico (CIC) y la concentración de Fe, además beneficiaron las propiedades físicas (densidades y porosidad) y proporcionaron un pH óptimo para la disponibilidad de P y Mg. En cuanto a los metales, presentaron en todos los tratamientos pérdidas por lixiviación de Ca, K y Mg y un aumento en la concentración de Fe.Item Study of titanium–silver monolayer and multilayer films for protective applications in biomedical devices(2021) Mina Aponzá, Sebastián; Castro Narváez, Sandra Patricia; Caicedo Bejarano, Luz Dary; Bermeo Acosta, FranklinThe search for coatings that extend the useful life of biomedical devices has been of great interest, and titanium has been of great relevance due to its innocuousness and low reactivity. This study contributes to the investigation of Ti/Ag films in different configurations (monolayer and multilayer) deposited by magnetron sputtering. The sessile droplet technique was applied to study wettability; greater film penetrability was obtained when Ag is the external layer, confer-ring high efficiency in cell adhesion. The morphological properties were characterized by SEM, which showed porous nuclei on the surface in the Ag coating and crystals embedded in the Ti film. The structural properties were studied by XRD, revealing the presence of TiO2 in the anatase crystalline phase in a proportion of 49.9% and the formation of a silver cubic network centered on the faces. Tafel polarization curves demonstrated improvements in the corrosion current densi-ties of Ag/Ti/Ag/Ti/Ag/Ti/Ag/Ti and Ti/Ag compared to the Ag coating, with values of 0.1749, 0.4802, and 2.044 nA.m−2, respectively. Antimicrobial activity was evaluated against the bacteria Pseudomonas aeruginosa and Bacillus subtilis and the yeasts Candida krusei and Candida albicans, revealing that the Ti/Ag and Ag/Ti/Ag/Ti/Ag/Ti/Ag/Ti coatings exhibit promise in biomedical material applications.Item Water Quality, Heavy Metals, and Antifungal Susceptibility to Fluconazole of Yeasts from Water Systems(Multidisciplinary Digital Publishing Institute (MDPI), 2023-02) Caicedo Bejarano, Luz Dary; Osorio Vanegas, Lizeth Stefania; Ramírez Castrillón, Mauricio; Castillo, Jorge Enrique; Martínez Garay, Carlos Andrés; Chávez Vivas, MónicaAquatic environments could be reservoirs of pathogenic yeasts with acquired antifungal resistance. The susceptibility to antifungal agents of yeasts present in the wastewater and natural waters of the city of Cali was evaluated. Samples were taken from two types of water: drinking water (Meléndez River, drinking water treatment plant “Puerto Mallarino” in the Cauca River) and wastewater (South Channel of the Cauca River, “Cañaveralejo-PTAR” wastewater treatment plant). Physico-chemical parameters, heavy metal concentration, and yeast levels were determined using standard procedures. Yeasts were identified using API 20 C AUX (BioMérieux) and sequence analysis of the ITS1-5.8S-ITS2 and D1/D2 regions of the large subunit of the ribosome. Susceptibility assays against fluconazole and amphotericin B using the minimum inhibitory concentration (MIC) test were determined using the microdilution method. The influence of physico-chemical parameters and heavy metals was established using principal component analysis (PCA). Yeast counts were higher at WWTP “PTAR” and lower at Melendez River, as expected. A total of 14 genera and 21 yeast species was identified, and the genus Candida was present at all locations. Susceptibility tests showed a 32.7% resistance profile to fluconazole in the order DWTP “Puerto Mallarino = WWTP “PTAR” > South Channel “Navarro”.