Facultad de Ciencias Básicas
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Browsing Facultad de Ciencias Básicas by Author "Alférez Osorio, Luisa Fernanda"
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Item Potencial farmacológico del extracto Diclorometánico de Piper Aduncum en la búsqueda de moléculas con actividad antimicobacteriana(Universidad Santiago de Cali, 2022) Alférez Osorio, Luisa Fernanda; Gómez Viera, Santiago; Aranaga Arias, Carlos Andrés (Director)Clinically important non-tuberculous mycobacteria (NTM) are a diverse group of microorganisms that cause a wide variety of infections in the skin, soft tissues, respiratory tract, among others. These mycobacteria present intrinsic and/or acquired resistance to a wide variety of antibiotics, requiring the development of new drugs for their control. A previous study conducted at the Universidad Santiago de Cali demonstrated the antimycobacterial potential of three plants of the genus Piper (P. aduncum L, P. auritum Kunth and P. peltatum L). In the present study, the pharmacological potential of Piper aduncum leaf dichloromethanolic extract was evaluated on clinical isolates of M. abscessus, M. massiliense, M. bolletii, M. fortuitum, M. chelonae and strain M. smegmatis mc2155 by broth microdilution method. The results show that the dichloromethanolic leaf extract of P. aduncum presents a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) between 128 and 512 µg/mL on the different clinical isolates used. In addition, the extract was fractionated by open column chromatography, obtaining 14 fractions of different polarities, demonstrating that the fractions of greater polarity (F12, F13 and F14) present inhibitory activity on M. smegmatis mc2155, obtaining a MIC and MIC between 136.50 and 929.50 µg/mL, possibly demonstrating the phenomenon of synergy between the bioactive compounds present in each fraction, which designates the mutual potentiation of several active principles. This study is expected to serve as a basis for the development of new antimycobacterial drugs.