Facultad de Ciencias Básicas
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Browsing Facultad de Ciencias Básicas by Author "Agudelo Sánchez, Luis Eduardo"
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Item Análisis del riesgo microbiológico asociado a coliformes fecales y Salmonella spp en el cultivo de rábano con aplicación de lodos residuales y biosólidos(Universidad Santiago de Cali, 2024) Peláez Giraldo, Angie Daniela; Agudelo Sánchez, Luis Eduardo; Cárdenas Talero, José Luis (Director)Domestic wastewater treatment plants generate by-products, including residual sludge and biosolids, which contain a large amount of organic matter and nutrients. This material is generally not utilized and is discarded without adding value, increasing its final disposal in landfills. Therefore, this research analyzed the microbiological risk associated with the use of residual sludge and biosolids applied to a radish crop (Raphanus sativus L.). Initial microbiological analyses (before treatment preparation) were conducted on the soil, sludge, and biosolids, as well as microbiological analyses of the treatments used at harvest, including the vegetable. The study was carried out using the most probable number (MPN) technique for the determination of fecal coliforms, based on Standard Methods, and the Colombian technical standard NTC 4574 was used to identify the presence of Salmonella spp. The results showed that the initial microbiological characteristics of biosolids A and B met the established regulations and could be used for the study. In the case of residual sludge, the microbial load was high because they are unstabilized by-products. However, microbiological results for the experimental treatments at the applied doses showed a significant decrease in microbial load for fecal coliforms and Salmonella spp. Nevertheless, radishes harvested from the different treatments, except for the treatment (T1: Soil-Biosolid A), presented high concentrations of fecal coliforms, making them unsuitable for consumption. The biosolids originated in the PTARca, when mixed with soil doses, generate a by-product with great potential for soil application, but it is not recommended to cultivate directly consumable foods as it was shown that microorganisms develop mechanisms to adhere to, survive, and multiply in the radish.