Facultad de Ciencias Básicas
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Browsing Facultad de Ciencias Básicas by Author "Agudelo Góngora, Helen Astrid"
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Item Evaluación de la actividad antibacteriana de un péptido catiónico frente a bacterias multirresistentes(Universidad Santiago de Cali, 2020) Agudelo Góngora, Helen Astrid; Rivera Sánchez, Sandra Patricia (Directora)The production of β-lactamase enzymes by some species of Enterobacteriaceae and non-fermenting bacteria is a serious public health problem, due to the fact that they cause the loss of therapeutic efficacy of β-lactam antibiotics, which are currently used as the first alternative for the management of infections caused by Gram negative bacteria. In this work, the antibacterial activity of a cationic peptide derived from Cecropin D from Galleria mellonella was evaluated against 15 clinical isolates of Klebsiella pneumoniae and Pseudomonas aeruginosa sensitive and 30 (15 P. aeruginosa and 15 K. pneumoniae) clinical isolates multiresistant phenotypically to different antimicrobial categories. In order to determine the peptide concentration required to inhibit these bacteria, the minimum inhibitory concentration (MIC) was determined using the Broth Microdilution method. The secondary verification of the method was carried out by conducting in vitro peptide assays and using the ISO 17025 and CLSI 2008 standards. Subsequently, MIC tests were carried out to evaluate the antibacterial activity of the peptide against sensitive K. pneumoniae and P. aeruginosa from 2 clinics in Cali, Valle del Cauca, Colombia and multi-resistant ones granted by the Departmental public health laboratory of Valle del Cauca. It was observed that 100% (30/30) of the sensitive clinical isolates were inhibited at peptide concentrations between 4 - 8 μg / mL and that 100% (30/30) of the multi-resistant isolates were inhibited at concentrations between 8 - 16μg / mL. Thus, the results suggest that the peptide has a high antibacterial capacity, therefore, knowing the antibacterial capacity of the peptide is an important step towards the development of new therapeutic alternatives to mitigate the spread and morbidity and mortality caused by these pathogens.