Química Farmacéutica
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Browsing Química Farmacéutica by Author "Aranaga Arias, Carlos Andrés (Director)"
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Item Caracterización fitoquímica del extracto etanólico de piper hispidum y evaluación in vitro de la actividad antimicobacteriana frente a bacterias del género Mycobacterium(Universidad Santiago de Cali, 2024) Mutiz, Naren Julián; González Arias, Catalina; Hernández, Jorge Enrique (Director); Aranaga Arias, Carlos Andrés (Director)The species Piper hispidum (Family Piperaceae) is a species native to Central and South America which is associated with various pharmacological activities such as anti-inflammatory, diuretic, antimicrobial, antifungal, healing, among others. The selection of this species for the work was based on reports of acrontimicrobial activity for plants of the piper genus. The plant material was collected in the Department of Cali, Valle del Cauca, where an ethanolic extract was prepared using the ultrasound-assisted maceration technique with a yield of 20%, where fractions of different polarities were obtained (petroleum ether, ethyl acetate, butanol and water). The extracts and their fractions were characterized Phytochemically and in relation to their in vitro activity against the strains Mycobacterium abscessus, M. fortitum, M. chelonae and M. smegmatis. The presence of phenolic groups, anthracene glycosides, coumarins, steroids and/or triterpenoids was detected, and the absence of alkaloids, saponins and cardiotonic glycosides was evident. In addition, chromatographic profiles were developed by high-efficiency liquid chromatography (HLC) of the extracts and fractions, which showed compounds of medium and high polarity. The antimycobacterial activity of the extracts and fractions was evaluated by the micro-dilution plate method, which revealed that the compounds contained in the ethanolic extract, as well as the petroleum ether and ethyl acetate fractions of Piper hispidum inhibited the growth at a concentration of 2 mg/mL against the mentioned mycobacteria and for the butanol and water fractions, growth greater than 2 mg/mL was evident.