The oleaginous yeast Meyerozyma guilliermondii BI281A as a new potential biodiesel feedstock: Selection and lipid production optimization
| dc.contributor.author | Ramírez-Castrillón, Mauricio | |
| dc.contributor.author | Jaramillo-García, Victoria P. | |
| dc.contributor.author | Rosa, Priscila D. | |
| dc.contributor.author | Landell, Melissa Fontes | |
| dc.contributor.author | Vu, Duong | |
| dc.contributor.author | Fabricio, Mariana F. | |
| dc.contributor.author | Ayub, Marco Antônio Záchia | |
| dc.contributor.author | Robert, Vincent A.R.G. | |
| dc.contributor.author | Henriques, João Antonio Pêgas | |
| dc.contributor.author | Valente, Patrícia | |
| dc.date.accessioned | 2019-07-08T22:15:28Z | |
| dc.date.available | 2019-07-08T22:15:28Z | |
| dc.date.issued | 2017-09-22 | |
| dc.description.abstract | A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast, Meyerozyma guilliermondii BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38% for glucose or 34.97% for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a biodiesel refinery. The lipid profile showed monounsaturated fatty acids (MUFA) varying between 56 or 74% in pure or raw glycerol, respectively. M. guilliermondii BI281A bears potential as a new biodiesel feedstock. © 2017 Ramírez-Castrillón, Jaramillo-Garcia, Rosa, Landell, Vu, Fabricio, Ayub, Robert, Henriques and Valente. | en_US |
| dc.identifier.issn | 1664302X | |
| dc.identifier.uri | https://repositorio.usc.edu.co/handle/20.500.12421/277 | |
| dc.language.iso | en | en_US |
| dc.publisher | Frontiers Media S.A. | en_US |
| dc.subject | Biodiesel | en_US |
| dc.subject | Fatty acids | en_US |
| dc.subject | Lipid production | en_US |
| dc.subject | Nile Red | en_US |
| dc.subject | Raw glycerol | en_US |
| dc.subject | Critical micelle concentration | en_US |
| dc.subject | Meyerozyma guilliermondii | en_US |
| dc.subject | Gas chromatography | en_US |
| dc.subject | Optical density | en_US |
| dc.subject | Nonhuman | en_US |
| dc.subject | Lipogenesis | en_US |
| dc.title | The oleaginous yeast Meyerozyma guilliermondii BI281A as a new potential biodiesel feedstock: Selection and lipid production optimization | en_US |
| dc.type | Article | en_US |
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