Water Quality, Heavy Metals, and Antifungal Susceptibility to Fluconazole of Yeasts from Water Systems
| dc.contributor.author | Caicedo Bejarano, Luz Dary | |
| dc.contributor.author | Osorio Vanegas, Lizeth Stefania | |
| dc.contributor.author | Ramírez Castrillón, Mauricio | |
| dc.contributor.author | Castillo, Jorge Enrique | |
| dc.contributor.author | Martínez Garay, Carlos Andrés | |
| dc.contributor.author | Chávez Vivas, Mónica | |
| dc.date.accessioned | 2025-07-04T19:29:28Z | |
| dc.date.available | 2025-07-04T19:29:28Z | |
| dc.date.issued | 2023-02 | |
| dc.description | Los ambientes acuáticos podrían ser reservorios de levaduras patógenas con resistencia antifúngica adquirida. Se evaluó la susceptibilidad a antifúngicos de levaduras presentes en aguas residuales y naturales de la ciudad de Cali. Se tomaron muestras de dos tipos de agua: potable (río Meléndez, planta de tratamiento de agua potable «Puerto Mallarino» en el río Cauca) y residual (Canal Sur del río Cauca, planta de tratamiento de aguas residuales «Cañaveralejo-PTAR»). Los parámetros fisicoquímicos, la concentración de metales pesados y los niveles de levaduras se determinaron utilizando procedimientos estándar. Las levaduras se identificaron usando API 20 C AUX (BioMérieux) y análisis de secuencia de las regiones ITS1-5.8S-ITS2 y D1/D2 de la subunidad grande del ribosoma. Se determinaron los ensayos de susceptibilidad frente a fluconazol y anfotericina B mediante la prueba de concentración inhibitoria mínima (CIM) utilizando el método de microdilución. La influencia de los parámetros fisicoquímicos y de los metales pesados se estableció mediante el análisis de componentes principales (ACP). Los recuentos de levaduras fueron mayores en la PTAR y menores en el río Meléndez, como era de esperar. Se identificaron un total de 14 géneros y 21 especies de levaduras, y el género Candida estuvo presente en todas las localizaciones. Las pruebas de susceptibilidad mostraron un perfil de resistencia al fluconazol del 32,7% en el orden ETAP «Puerto Mallarino = EDAR “PTAR” > Canal Sur »Navarro". | |
| dc.description.abstract | Aquatic environments could be reservoirs of pathogenic yeasts with acquired antifungal resistance. The susceptibility to antifungal agents of yeasts present in the wastewater and natural waters of the city of Cali was evaluated. Samples were taken from two types of water: drinking water (Meléndez River, drinking water treatment plant “Puerto Mallarino” in the Cauca River) and wastewater (South Channel of the Cauca River, “Cañaveralejo-PTAR” wastewater treatment plant). Physico-chemical parameters, heavy metal concentration, and yeast levels were determined using standard procedures. Yeasts were identified using API 20 C AUX (BioMérieux) and sequence analysis of the ITS1-5.8S-ITS2 and D1/D2 regions of the large subunit of the ribosome. Susceptibility assays against fluconazole and amphotericin B using the minimum inhibitory concentration (MIC) test were determined using the microdilution method. The influence of physico-chemical parameters and heavy metals was established using principal component analysis (PCA). Yeast counts were higher at WWTP “PTAR” and lower at Melendez River, as expected. A total of 14 genera and 21 yeast species was identified, and the genus Candida was present at all locations. Susceptibility tests showed a 32.7% resistance profile to fluconazole in the order DWTP “Puerto Mallarino = WWTP “PTAR” > South Channel “Navarro”. | |
| dc.identifier.issn | 16617827 | |
| dc.identifier.uri | https://repositorio.usc.edu.co/handle/20.500.12421/7177 | |
| dc.language.iso | en | |
| dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | |
| dc.subject | antifungals | |
| dc.subject | drinking water | |
| dc.subject | heavy metals | |
| dc.subject | susceptibility | |
| dc.subject | wastewater | |
| dc.subject | water quality | |
| dc.subject | yeasts | |
| dc.title | Water Quality, Heavy Metals, and Antifungal Susceptibility to Fluconazole of Yeasts from Water Systems | |
| dc.type | Article |