Detection of carbapenemase-producing Pseudomonas aeruginosa: Evaluation of the carbapenem inactivation method (CIM)
| dc.contributor.author | Gutiérrez, Sergio | |
| dc.contributor.author | Correa, Adriana | |
| dc.contributor.author | Hernández Gómez, Cristhian | |
| dc.contributor.author | De La Cadena, Elsa | |
| dc.contributor.author | Pallares, Christian | |
| dc.contributor.author | Villegas, María Virginia | |
| dc.date.accessioned | 2020-02-09T18:45:36Z | |
| dc.date.available | 2020-02-09T18:45:36Z | |
| dc.date.issued | 2019-03-18 | |
| dc.description.abstract | Introduction: The carbapenem inactivation method (CIM) is a cost-effective assay for detecting carbapenemases. However, its interpretation is unclear for Pseudomonas spp. We evaluate its accuracy when meropenem is changed to imipenem. Methods: We analyzed 266 P. aeruginosa isolates. The CIM method consists of: resuspend bacterial colonies (a full 10 μL loop) in 400 μL water, in which a 10 μg disk of meropenem/imipenem is immersed. After 2 h of incubation (35 °C), remove the disk, place it onto a Mueller-Hinton agar plate previously inoculated with Escherichia coli (ATCC 25922), and incubate at 35 ̊C between 18-24 h. Interpretation criteria (mm of inhibition zone): ≤19 mm, positive; ≥25 mm negative; 20–24 mm, undetermined. Results: Imipenem improves the sensitivity and specificity of CIM when compared to meropenem (99.4% and 98.9%, vs. 91.9% and 94.7%, respectively). Conclusions: The accuracy of CIM for carbapenemase detection in P. aeruginosa is increased with the use of imipenem. | es |
| dc.identifier.issn | 0213005X | |
| dc.identifier.uri | https://repositorio.usc.edu.co/handle/20.500.12421/2638 | |
| dc.language.iso | en | es |
| dc.publisher | Elsevier Doyma | es |
| dc.subject | Carbapenemases | es |
| dc.subject | Screening test | es |
| dc.subject | Carbapenemases phenotypic detection | es |
| dc.subject | Carbapenem inactivation method | es |
| dc.subject | Pseudomonas aeruginosa | es |
| dc.title | Detection of carbapenemase-producing Pseudomonas aeruginosa: Evaluation of the carbapenem inactivation method (CIM) | es |
| dc.type | Article | es |
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