Effects of pH and application technique of in-office bleaching gels on hydrogen peroxide penetration into the pulp chamber

dc.contributor.authorBalladares, Luis
dc.contributor.authorAlegría Acevedo, Luisa
dc.contributor.authorMontenegro, Andres
dc.contributor.authorArana Gordillo, Luis Alfonso
dc.contributor.authorPulido, Camilo A
dc.contributor.authorSalazar Gracez, María Teresa
dc.contributor.authorReis, Alessandra
dc.contributor.authorLoguercio, Alessandro D
dc.date.accessioned2020-02-10T01:03:30Z
dc.date.available2020-02-10T01:03:30Z
dc.date.issued2019-11-13
dc.description.abstractObjective: This in vitro study aimed to quantify the penetration of hydrogen peroxide (HP) into the pulp chamber in teeth submitted to in-office bleaching with varied pH and application techniques. The color change and pH of the in-office bleaching product during application was also evaluated. Methods and Materials: Ninety-six human premolars were used and randomly divided into 10 groups (n=9) according to the following combination of factors: pH of in-office bleaching agents (two neutral/alkaline pH: Opalescence Boost 38% and Whiteness HP Blue 35% and three acidic pH: Whiteness HP Maxx 35%, Lase Peroxide Sensy 35%, and Total Blanc Office 35%) and application modes (for 3 × 15 minutes [3×15] and 1 × 45 minutes [1×45]). An additional group of non-bleached teeth (control; n=6) was added. First, all teeth were sectioned 3 mm from the cementoenamel junction and the pulp tissue was removed. An acetate buffer was placed in the pulp chamber of all teeth. After bleaching, this solution was transferred to a glass tube in which HP was allowed to react with other components, resulting in a pink solution. The optical density of this pink solution was measured using ultraviolet-visible spectroscopy and converted into amount of HP. Color change before and 1 week after bleaching was evaluated using a digital spectrophotometer. A pH meter with a 6-mm circular and flat surface was used in contact with the enamel surface to quantify the pH of the bleaching gels during application. Data were analyzed using two-way analysis of variance and Tukey tests (α=0.05). Results: Overall, lower mean HP penetration values were observed for Opalescence Boost 38% and Whiteness HP Blue 35% compared with other bleaching gels (p<0.05). Opalescence Boost 38% and Whiteness HP Blue 35% were not influenced by the application technique (p>0.05). However, lower mean HP penetration values were observed for Whiteness HP Maxx 35%, Total Blanc Office 35%, and Lase Peroxide Sensy 35% when using the 3×15 application technique compared with the 1×45 technique (p<0.05). Significant whitening was detected and no significant difference of color change was observed between groups (p>0.54). The pH did not change during the 3×15 application technique; however, all acidic bleaching gels significantly decreased in pH when applied for 1×45 (p<0.01). Conclusions: The amount of HP that reaches the pulp chamber was lower when neutral/alkaline pH gels were used, independently of the application technique. When considering acidic pH gels, it is preferable to use the 3×15 application technique, mainly because longer application time (1×45) results in lower pH. No difference was observed between groupses
dc.identifier.issn1559-2863
dc.identifier.urihttps://repositorio.usc.edu.co/handle/20.500.12421/2694
dc.language.isoenes
dc.publisherIndiana University School of Dentistryes
dc.subjectHydrogen peroxidees
dc.subjectTooth bleaching agentes
dc.subjectDental Pulp Cavityes
dc.subjectGelses
dc.subjectHydrogen Peroxidees
dc.titleEffects of pH and application technique of in-office bleaching gels on hydrogen peroxide penetration into the pulp chamberes
dc.typeArticlees

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