Browsing by Author "Samboni Tenorio, Alejandro"
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Item Evaluación del efecto inhibitorio de los pigmentos extraídos de Aureobasidium spp frente a Lactobacillus spp. aislados de la fermentación alcohólica(Universidad Santiago de Cali, 2022) Samboni Tenorio, Alejandro; Parrado, Darly Silvana; Aranaga Arias, Carlos AndrésMelanin is one of the most widely distributed pigments in nature and among other functions, it has been attributed to a great antibacterial activity. In the present study, the production of the melanin-type pigment was determined in crude extracts obtained from four environmental isolates of yeast-like fungi of the genus Aureobasdium (LC 112, YAM1, YAM2 and YAM4), as potential inhibitors of bacteria of the genus Lactobacillus contaminating the alcoholic fermentation and the yeast of Saccharomyces cerevisiae alcohol producer. For the production of the pigment, the Aureobasdium isolates were grown in an inducing medium (KH2PO4 0.1%, (NH4)2 SO4 0.05%, MgCl2-6H2O 0.05% and glucose 5%), with constant agitation for 120 hours. To obtain the extract, the cells were harvested, lysed and acetone was used as extraction solvent. Once the extracts were obtained, the Minimum Inhibitory Concentration (MIC) was determined by microdilution assay in broth with resazurin. The results show a higher extraction efficiency in the isolate YAM2 (2.1 g/L) followed by YAM1 (1.66 g/L), YAM4 (1.06 g/L) and LC112 (1.03 g/L).), while the MIC for all tested microorganisms was greater than 2 mg/ml. Since no extract showed antimicrobial activity at the maximum concentration established in this work, it is proposed to quantify the concentration of melanin present in each extract. In this way, a theoretical concentration could be established to carry out microbial inhibition tests that allow establishing the real potential of the use of Aureobasdium extracts in the inhibition of contaminating microorganisms in the alcoholic industry