Browsing by Author "Flórez Elvira, Liliana Janeth"
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Item High Prevalence of Salmonella spp. in Ready-to-Eat Artisanal Pork Sausages Sold at Food Outlets in Quindío, Colombia(Multidisciplinary Digital Publishing Institute (MDPI), 2025-01-04) Jaramillo Bedoya, Elizabeth; Flórez Elvira, Liliana Janeth; Ocampo Ibáñez, Iván DaríoNon-typhoidal salmonellosis is a foodborne disease caused by Salmonella spp. Most outbreaks of this disease are commonly associated with consuming contaminated meat products, hence the importance of monitoring ready-to-eat artisanal pork sausages for the presence of these bacteria. A total of 494 samples of grilled and smoked barbecue artisanal pork sausages were collected at food outlets from 12 municipalities of the Department of Quindío, Colombia, between 2017 and 2022. Salmonella spp. was identified using VIDAS® Easy SLM and confirmed through API® 20 E. Salmonella spp. was detected in 260 samples (52.6%), and the highest rates of contamination were found in Armenia (65.7%), Salento (65.2%), Circasia (57.7%), and Calarcá (56.4%). The highest proportion of these samples positive for Salmonella spp. was ready-to-eat smoked barbecue artisanal sausages (68.8%) sold by street vendors (58.4%) from 12 municipalities of the Department of Quindío. A significant association was observed between the municipality and contamination of samples with Salmonella spp. However, no link was found between the sampling year and the presence of the bacteria. This is the first study aimed at monitoring the presence of Salmonella spp. in artisanal pork sausages sold in the municipalities of the Department of Quindío over a 6-year period, and findings revealed very high percentages of contamination. Although Colombian legislation establishes sanitary and safety requirements for meat production, the presence of Salmonella spp. in artisanal sausages remains a persistent public health threat in developing countries.Item Presence of Listeria monocytogenes in fresh artisanal cheese marketed in Cali-Colombia(Universidad Nacional de Colombia, 2019-04-09) Ocampo Ibáñez, Iván Darío; González, Carlos; Moreno, Sara Lucia; Calderón, Cristina; Flórez Elvira, Liliana Janeth; Beatriz Olaya, María; Rivera Sánchez, Sandra Patricia; Lesmes, María CristinaListeria monocytogenes is the causative agent of the foodborne illness called listeriosis. The invasive form of this disease can cause meningoencephalitis, septicemia and spontaneous abortions. The consumption of food contaminated with this bacterium is the main route of infection, and the fresh artisanal cheeses cause the greatest concern in public health. In this study, different types of fresh artisanal cheeses (campesino, costeño, cuajada, doble crema, mozarella and pastuso) were collected in market squares in Cali (Alameda, El Cortijo, La Floresta and Santa Elena) and analyzed using the VIDAS® and VITEK®2 platforms to detect and identify L. monocytogenes. According to the results obtained from 126 fresh artisanal cheeses analyzed in the different market squares, 27% were positive for L. monocytogenes. Among the positive samples, cuajada had the highest number of positive samples for L. monocytogenes with 44.1%. Alameda with 62% of positive samples for L. monocytogenes had the highest number of positive samples among all market squares evaluated. This study represents the first evaluation of the presence of L. monocytogenes in fresh artisanal cheeses marketed in the most important market squares of Cali. Accordingly, this study pretends to generate an alert about the urgent need to implement surveillance and control mechanisms of L. monocytogenes in the manufacture and commercialization of food, considering that Colombian legislation does not currently establish the obligatory for surveillance of L. monocytogenes in fresh cheeses. © 2019 Universidad Nacional de Colombia. All rights reserved.