Browsing by Author "Cogua Romero, Rosa del Pilar (Directora)"
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Item Determinación de la vigencia, la estabilidad y funcionalidad de medios de cultivo selectivos, no selectivos y fluidos de transporte en un laboratorio de microbiología farmacéutica(Universidad Santiago de Cali, 2025-11-26) Ordoñez Casadiegos, Jose Ricardo; Cogua Romero, Rosa del Pilar (Directora)Determining the shelf life of culture media is essential in the pharmaceutical industry, as it guarantees the reliability of microbiological results and compliance with international standards such as ISO 11133, ISO 17025, and USP <61>–<62>. In response to the dependence on imported media and the need to optimize resources, this study aimed to establish the period of use, stability, and functionality of solid, liquid, and transport media prepared in a microbiological quality control laboratory of a pharmaceutical company. The media were prepared under controlled conditions following the specifications of international pharmacopoeias. Physicochemical parameters such as pH, color, texture, and appearance were evaluated, as well as microbiological parameters such as growth promotion, selective inhibition, and recovery, using certified reference strains. ANOVA and Tukey's post-hoc tests were performed to determine the existence of significant variations between the different evaluation times (days 1, 15, and 30). The results showed that all media retained their physical appearance and pH stability, with variations of less than 0.2 units. In growth promotion tests, non-selective and selective media and transport fluids showed recovery rates of over 70%, meeting the acceptance criteria.