Browsing by Author "Caicedo Grueso, Kelly Vanessa"
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Item Aislamiento y cuantificación de microorganismos nativos con capacidad celulolítica y amilolítica presentes en el bagazo y la cachaza empleados como materias primas utilizadas en la producción de abonos orgánicos, procedentes de un ingenio azucarero del Valle del Cauca(Universidad Santiago de Cali, 2024) Caicedo Grueso, Kelly Vanessa; Oviedo Mossos, Angela (Directora); Corredor Sáenz, Viviana (Directora)The study characterized the native microbial population with cellulolytic and amylolytic capacity present in bagasse and press mud, by-products of a sugar mill in Valle del Cauca for the production of organic fertilizers. To quantify the hydrolytic activity of the isolates present in bagasse and press mud, they were evaluated following the methods of Teather and Wood, as well as that of Lugol, using culture media with carboxymethylcellulose (CMC) and starch at 1% (w/v). The investigation revealed that the press mud harbors a greater microbial diversity, with a cellulolytic bacterial population (50%), while in the bagasse, cellulolytic bacteria (46%) predominated over amylolytic bacteria. Serial dilutions of 10-6 and 10-7 were optimal for the isolation of cellulolytic microorganisms in both substrates. In terms of enzymatic activity, bagasse showed higher amylolytic activity (7.70 Lg UCF/g), while press mud stood out in cellulolytic activity (7.70 Lg CFU/g). These findings contribute to the knowledge of the potential in the bioconversion of agroindustrial by-products for their use in the production of more efficient organic fertilizers.