USC Repository :: Browsing by Author "Vivas Parra, Aura Elena"

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Mejoramiento de la producción de carotenoides en la cepa Rhodotorula mucilaginosa AJB01 mediante evolución adaptativa en laboratorio
(Universidad Santiago de Cali, 2021) Vivas Parra, Aura Elena; Ramírez Castrillón, Mauricio; Dávalos Vélez, Andrés Felipe
Adaptive laboratory evolution or evolutionary engineering, has been used for many decades to enhance critical features of industrial strains, such as carbon source utilization, tolerance to adverse environmental conditions and resistance to chemical inhibitors, due to its simplicity and effectiveness. In this investigation, an evolutionary directed experiment was carried out in order to improve total carotenoid production in R. mucilaginosa AJB01, by using an oxidative stress as the selective pressure, with increasing periodic doses of hydrogen peroxide (H2O2). The performed experiment allowed a sustained selection of higher carotenoid-producing phenotypes, surpassing the parental concentration of 170,0 mg/g in 20,1% with a total of 205,8 mg/g. This study represents the first approach in the developement of an improvement model for the production of carotenoids in yeasts belonging to Rhodotorula. Based on the achieved results, the continuation of the developed model promises a high potential in the acqusition of widely adapted higher carotenoid-producing phenotypes