Browsing by Author "Vidal Arango, Karen Johana"
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Item Evaluación de bacterias potencialmente degradadoras de polietileno de baja densidad (LDPE)(Universidad Santiago de Cali, 2022) Vidal Arango, Karen Johana; Bomba Ospina, María Isabel; Álzate Calderón, PaolaLow-density polyethylene (LDPE) is a material used to wrap products and to produce packaging. The prolonged use of this material and the inadequate management of the waste generated contribute to the environmental contamination of the planet. The objective of the present study is to find at least one bacterium potentially degrading LDPE-type plastic. The strains were randomly selected, reactivated in nutrient agar by depletion seeding and in vitro assays were carried out with the study material for approximately six months. The plastics used as matrix to evaluate degradation were from the packaging of the following commercial brands: Rendy (toilet paper), Mercamio (bread), Familia (napkins), Diana (rice), Control (ziploc). The results obtained showed that there was an important deterioration in the plastics of the Rendy brand, where 24,0% degradation was evidenced. Gram staining and sowing in the selective media Agar Bacillus and Agar Cetrimide verified the growth of colonies with the typical morphology for each culture medium. Finally, an analysis was carried out in the TEMPO® equipment to identify the bacteria with the greatest degradation potential, which yielded the microorganism Bacillus cereus as the greatest degrader of plastic. It is concluded that this study is of vital importance since it contributes with an alternative that would allow reducing the contamination caused by petroleum-derived plastics and opens doors for future research in the area, where it is expected that more microorganisms with great degradation potential can be found