Browsing by Author "Varela Miranda, Rubén Eduardo (Director)"
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Item Identificación de péptidos aislados del caracol gigante africano (Achatina fulica) y estudio de su actividad antimicrobiana(Universidad Santiago de Cali, 2020) Martínez Esquivel, Richard Andrés; Varela Miranda, Rubén Eduardo (Director); Oñate Garzón, Jose Fernando (Director)Snails, due to their potential in bioprospecting, have been studied in different settings, one of which is obtaining peptides with therapeutic activity from African snail slime, since antitumor and cosmetic peptides have been identified in this species. The rapid spread of antibiotic-resistant microorganisms in recent decades and the poor development of new antimicrobial drugs has represented a significant public health problem worldwide. Therefore, it is necessary to look for alternatives for the design of new drugs, for example, antimicrobial peptides from snail slime type (PAMs). For the extraction of peptides from the African snail slime, the TRI Reagent®, reagent was used as a total protein extraction method, with a previous treatment with N-acetylcysteine for the elimination of the mucin present in the slime and thus improve the sample quality. Subsequently, the proteins extracted and filtered with AMICON were visualized with SDS gels with tricine at 14 and 16%, obtaining small bands mainly below 10 KDa, confirming the presence of peptides, despite this, the extract evaluated did not show antimicrobial activity. Finally, the data from the CIC (Cancer Research Center) of Spain also reported the isolation of peptides in the samples; however, its identification and synthesis are pending for the evaluation of its antimicrobial activity with pure peptides and in adequate concentrations. These results validate that the methodology used is appropriate for the isolation of peptides from the African snail slime in isolates from the city of Cali.Item Síndrome post covid-19, implicaciones funcionales y factores relacionados(Universidad Santiago de Cali, 2024-08-23) Daza Arana, Jorge Enrique; Varela Miranda, Rubén Eduardo (Director)COVID-19 is a viral infection that has generated a high burden of morbidity and mortality during the pandemic worldwide, becoming a disabling disease in the population that persists with symptoms after 12 weeks of SARS-CoV infection. 2 and that no alternative diagnosis is attributed. The functional implications of Post COVID-19 Syndrome include the domains of functional impairment, fatigue, quality of life, mental and/or cognitive function problems, and low exercise capacity. Considering that there are only three studies in the country in relation to this syndrome, it was necessary to investigate the municipality of Palmira because it is one of the territories with the highest number of cases in the department of Valle del Cauca and one of those that has greater lethality. A descriptive observational epidemiological study with an analytical component was carried out that allowed us to describe and know the frequency of persistent symptoms, the functional implications and the burden of the affected systems, in relation to the sociodemographic and clinical characteristics. It was found that the population was mainly female, middle and low socioeconomic stratum, had a single event of COVID-19, more than 5 symptoms in the acute stage and received outpatient management. The majority presented a complete vaccination schedule against COVID-19, but only 26.9% had vaccination prior to infection. It was evident that cognitive symptoms represented 88.5% of the persistent symptoms, followed by general (84.6%), musculoskeletal (82.7%) and neurological symptoms (75.8%). In functional status, 70% present minimal to severe limitations. Chronic fatigue had a prevalence of 36.5% (38/104). Regarding quality of life, it was observed that half (50.0%) of the population reports a mild to moderate level of pain or discomfort and around a third (34.6%) a level of anxiety and depression. Cognitive mental function was found with 55.8% at a reduced level. The most affected areas were memory with 88.5%, followed by the visuospatial/executive area with 71.2% and attention 61.5%. It was evidenced that a single episode of acute COVID-19, without critical illness, can develop Post-COVID-19 Syndrome and subsequent vaccination does not seem to be a protective factor. After evaluating the results obtained in this population, the implementation of prevention and management strategies is recommended that facilitate comprehensive rehabilitation in a timely manner with the aim of reducing functional implications.