Repository logo
  • English
  • Español
  • Log In
    New user? Click here to register. Have you forgotten your password?
Repository logo
  • Communities & Collections
  • All
  • English
  • Español
  • Log In
    New user? Click here to register. Have you forgotten your password?
  1. Home
  2. Browse by Author

Browsing by Author "Ramirez Castrillón, Mauricio"

Now showing 1 - 1 of 1
Results Per Page
Sort Options
  • No Thumbnail Available
    Item
    Biodecolourisation and biodegradation of leather dyes by a native isolate of Trametes villosa
    (Institution of Chemical Engineers, 2017-05-04) Ortiz Monsalve, Santiago; Dornelles, Juliana; Poll, Eduardo; Ramirez Castrillón, Mauricio; Valente, Patricia; Gutterres, Mariliz
    Dyeing is an important step in the leather manufacture process. Effluent from this stage contains some types of synthetic dye that may be a threat to the environment and human health. Biological treatment of dye-containing wastewaters by microorganisms has been presented as a cost effective and promising environmentally friendly alternative. In the present work, the potential of Brazilian native white-rot fungi strains, collected and screened to produce extracellular ligninolytic enzymes, was evaluated for the biodecolourisation and biodegradation of different azo tannery dyes. The strain SCS-10 showed high activity of ligninolytic enzymes and allowed the colour removal of dyes in solid media. This isolate was characterised morphologically and identified as Trametes villosa, based on a molecular analysis of the internal transcribed spacer (ITS) region sequences. T. villosa SCS-10 showed high biodecolourisation efficiency for the dyes assessed, achieving 95.71 ± 1.29, 92.76 ± 0.99 and 96.84 ± 1.39% for Acid Red 357, Acid Black 210 and Acid Blue 161, respectively, at 100 mg L−1, 30 °C, pH 5.5 and 150 rpm, within 168 h of treatment. Remarkable peaks of laccase activity (1150–1550 U L−1) were observed during specific periods in the biodecolourisation process. The complete inhibition of Lac activity by sodium azide (NaN3, 0.1 mM) led to biodecolourisation values of 13.29 ± 0.93, 12.30 ± 0.46 and 20.05 ± 2.08% for AR357, AB210 and AB161, respectively. These results confirmed the main role of laccase in colour removal, although biosorption also had a minor involvement in biodecolourisation. In vitro assays also showed the efficiency of decolourisation of the leather dyes. The enzymatic crude extract produced by T. villosa allowed 85.45 ± 3.43 (AR357), 76.96 ± 1.39 (AB210) and 90.17 ± 0.97% (AB161) of biodecolourisation when enhanced by the use of the redox mediator 1-hydroxybenzotriazol (HBT, 1 mM). UV–vis and FTIR spectral analyses confirmed the occurrence of enzymatic biodegradation as the mechanism responsible for colour removal. T. villosa SCS-10 was able to tolerate high concentrations of the dyes (200–1000 mg L−1) and a wide range of pH (4.0–8.0) during biodecolourisation. The native isolate T. villosa SCS-10 is considered a suitable candidate for the treatment of dye-polluted wastewater from the leather industry due to the mechanisms of enzymatic biodegradation and biosorption.

Higher Education Institution subject to inspection and surveillance by the Ministry of National Education.
Legal status granted by the Ministry of Justice through Resolution No. 2,800 of September 2, 1959.
Recognized as a University by Decree No. 1297 of 1964 issued by the Ministry of National Education.

Institutionally Accredited in High Quality through Resolution No. 018144 of September 27, 2021, issued by the Ministry of National Education.

Ciudadela Pampalinda

Calle 5 # 62-00 Barrio Pampalinda
PBX: +57 (602) 518 3000
Santiago de Cali, Valle del Cauca
Colombia

Headquarters Centro

Carrera 8 # 8-17 Barrio Santa Rosa
PBX: +57 (602) 518 3000
Santiago de Cali, Valle del Cauca
Colombia

Palmira Section

Carrera 29 # 38-47 Barrio Alfonso López
PBX: +57 (602) 284 4006
Palmira, Valle del Cauca
Colombia

DSpace software copyright © 2002-2025 LYRASIS

  • Cookie settings
  • Privacy policy
  • End User Agreement
  • Send Feedback

Hosting & Support