Browsing by Author "Polo Osorio, Edgar Allan"
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Item Extracción de compuestos fitoquímicos de Tradescantia zebrina y de Juglans neotropica mediante combinación de microextracción en fase sólida y un dispositivo de disco rotatorio(Universidad Santiago de Cali, 2024) Polo Osorio, Edgar Allan; López Parra, Lizbeth Lorena (Directora); Ríos Acevedo, John Jairo (Director)In this research, a novel technique was applied to separate phytochemicals based on the combination of Solid Phase MicroExtraction (SPME) and a rotary disk device (Rotating Disk Sorptive Extraction - RDSE), on the Juglans neotropica (walnut) and Tradescantia zebrina (Panamanian) plants. In order to find the phytochemicals of these plants, the analysis method of gas chromatography coupled to mass spectrometry (GCMS) was used. Thus, the methodology used consisted of first finding out the situable solvent, which corresponding to the principles of green chemistry, that is, ecological, and second, probing the optimal extraction conditions, specifically: temperature, agitation and time. Therefore, the solvents tryed out were: ethanol, methanol, water and mixtures of ethanol-water and methanol-water. The leachates of both plants were obtained in methanol as a suitable solvent, in 60 minutes, 40°C and 600 rpm. In such leachates the main components for Juglans neotropica were: juglone, neophytadiene and farnesene; and the main components, for Tradescantia zebrina were: neophytadiene and phytol. Regarding the extraction with SPME, methanol-water (70-30) was better as a suitable solvent, also in 60 minutes, 40°C and 600 rpm, with which, for Juglans neotropica, the following were extracted: nerolidol, phytol, geranil acetone, caryophyllene, squalene, beta elemene and palmitic acid. For Tradescantia zebrina, the following were extracted: nerylacetone, neophytadiene, squalene, methyl linoleate, methyl linolenate and palmitic acid. Finally, Juglans neotropica and Tradescantia zebrina were used for a preliminary test of biological activity on Pseudomonas aeruginosa, verifying that no antimicrobial activity was produced. The fundamental contribution of this work is the information obtained from the application of the combination of RDSE-SPME-GC-MS on Juglans neotropica and Tradescantia zebrina.