Browsing by Author "Peralta Loboa, Camilo"
Now showing 1 - 1 of 1
Results Per Page
Sort Options
Item Evaluación de la actividad antimicrobiana de péptidos obtenidos a partir de la hidrólisis enzimática de la proteína de Chenopodium quinoa frente a Staphylococcus aureus y Escherichia coli(Universidad Santiago de Cali, 2023) Penagos Montaña, Nataly; Peralta Loboa, Camilo; Oñate Garzón, José Fernando; Cardona Cardona, Yudy VanesaPeptides are amino acid polymers with bioactive properties. In this regard, quinoa (Chenopodium quinoa) is a good alternative for peptide production due to its high protein composition and abundance in the national territory. However, the high cost of synthesis has led to the search for other alternatives such as enzymatic hydrolysis, where commercial enzymes obtained from plants, like actinidin, a cysteine-protease present in kiwi (Actinidina deliciosa), with proteolytic activity on vegetable substrates, are used. In this study, enzymatic hydrolysis of quinoa protein was performed using actinidin after an alkaline extraction. The obtained peptides were quantified using the Bradford method. Finally, their antimicrobial activity against S. aureus ATCC 25923 and E. coli ATCC 25922 was evaluated. The results indicated that the obtained peptides exhibited inhibition up to 0.000509 mg/mL for S. aureus ATCC 25923 and 0.004008 mg/mL for E. coli ATCC 25922