Browsing by Author "Pantoja Pulido, Kriss Dayana (Directora)"
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Item Extracción de alcaloides presentes en las semillas de Lupinus mutabilis y su actividad antibacteriana(Universidad Santiago de Cali, 2020) Martínez Correa, Kelly Alejandra; Acosta Quiñonez, Roberth Yair; Pantoja Pulido, Kriss Dayana (Directora); López Parra, Lizbeth Lorena (Directora)Lupinus mutabilis is a plant with high nutritional properties and high agronomic potential derived from the protein content of its seeds and growing conditions in the soil. This plant biosynthesizes different secondary metabolites with antioxidant and antibacterial properties, making it potential for the development of antibacterial products. Due to the above, this research aims to extract alkaloids present in Lupinus mutabilis seeds grown in Valle del Cauca, to evaluate their antibacterial activity and compare them with seeds of the same type grown in Ecuador. For this, the Liquid-liquid acid extraction of the seeds was carried out and the presence of flavonoids, steroids, saponins and alkaloids was determined by phytochemical screening. As the main result, lupanin was evidenced as the most abundant alkaloid, followed by sparteine and 3β-hydroxylupanine. In addition, it was found that the crude alkaloid extract of seeds grown in Valle del Cauca, presents more alkaloid content 95.8% compared to 50.0% of seeds grown in Ecuador.Item Identificación y evaluación de actividad antibacteriana contra Staphylococcus aureus de Metabolitos Secundarios a partir de Extractos de Eunicea sp y Plexaurella sp(Universidad Santiago de Cali, 2020) Mejía Gómez, Diego Alejandro; López Parra, Lizbeth Lorena (Directora); Pantoja Pulido, Kriss Dayana (Directora)The purpose of this work is to identify metabolites with antibacterial activity from two species of octocorals of the genus Eunicea and Plexaurella collected in Providencia, Colombia. For the extraction of metabolites, two solvents (methanol and hexane) were used together with the use of a rotary evaporator and column fractionation. The identification of metabolites was carried out through the application of liquid chromatography coupled to mass spectrometry, where it was seen that the compounds found were mostly fatty acids. The antibacterial activity was carried out by the sensidisk method, using a chloramphenicol disc as a positive control, where finally inhibition of Staphylococcus aureus could be seen in the complete extract.Item Moluscos de el Charco Nariño: Una mirada desde la exploración bioquímica(Universidad Santiago de Cali, 2020) Caicedo Perlaza, Yaneth Angélica; Nieto Ramírez, Luisa María (Directora); Pantoja Pulido, Kriss Dayana (Directora)Mollusks are non-segmented soft-bodied invertebrates protected by a shell and constitute the basis of the diet of many communities. Our objective was to determine the biochemical composition of two species of mollusks Littorina zebra (piacuil) and Anadara tuberculosa (piangua), which are an important nutritional source in El Charco, Nariño. In total, 30 samples of L. zebra with average sizes of approximately 3cm were collected, and 30 samples of A. tuberculosa differentiated by sex, identifying 13 females with average lengths of 8.4cm and 17 males with average lengths of 6.3cm. Mud samples were also collected from the mollusk habitat, from which the percentage of humidity and pH was determined. The bodies of the mollusks were separated from their shells and then lyophilized to carry out the determination of proteins by the method of BCA (Bicinconinic Acid) and polyacrylamide electrophoresis, extraction of total lipids by the Folch method and evaluation of these by layer chromatography fine (TLC). Finally, the total DNA extraction was carried out using a commercial kit and the quantification and evaluation of its quality using the Nanodrop equipment. In the soil samples a humidity of 23.4% and a pH of 6.25 were observed on average. An average value of total proteins of 3.27 g (± 0.65) was obtained for male A. tuberculosa, 3.73 g (± 0.14) for female A. tuberculosa and 4.69 g (± 0.11 ) for L. zebra per 100 g of lyophilized tissue, values that are lower than those reported in the literature. The amount of total lipids extracted was determined, obtaining an average of 2.1 g (± 0.8) for L. zebra, 2.45 g (± 0.3) for female A. tuberculosa and 2.35 g (± 0.7) for Male A. tuberculosa per 100 g of lyophilized tissue, values very similar to those found in the literature. A qualitative evaluation of lipids was carried out by TLC (Thin Layer Chromatography), where the presence of colored bands that demonstrated the oxidation of organic compounds and the presence of active compounds was observed. Finally, the DNA for L. zebra and A. tuberculosa male was successfully extracted, however, for the female it was not possible to obtain a DNA solution of satisfactory quality, for which the use of another methodology is recommended to eliminate pigments that affect Nanodrop quantification. This work was able to corroborate the ancestral knowledge of the Pacific communities that this type of species has a high protein content, particularly the L. zebra species and nutritional value; Therefore, it will be possible to bring documented information to the communities that allows them to validate their ancestral beliefs and to make good use of these natural resources.Item Síntesis de análogos del derivado dibenzotiofenoimidazol-UBMC como potenciales moléculas anti-Leishmaniasis mediante reacciones de acoplamiento con Pd(Universidad Santiago de Cali, 2023) Triana Muñoz, Andrés Camilo; Pantoja Pulido, Kriss Dayana (Directora); Flórez López, Edwin (Director)The products obtained in this research work were 4,5-bis (dibenzo [b, d] thiophen-2-yl) -2-phenyl-1H-imidazole (4) and 1,4,5-tris (dibenzo [b, d] thiophen-2-yl) -2-phenyl-1H-imidazole (6) which were synthesized under the reaction of Suzuki Miyaura in 3 different methodologies; Conventional heating ∆, US ultrasound and MW microwaves; obtaining 12.12%, 11.13% and 10.13% yield for the product (4) in the ∆, US and MW methodologies respectively and 5.18%, 30.23% and 8.27% for the product (6) under the same methodologies respectively. These products were characterized by mass spectrophotometry, infrared (IR) and nuclear magnetic resonance (NMR) of carbon 13 (13C), hydrogen (1H), COSY, HMBC and HSQC.