Browsing by Author "Osorio Vanegas, Lizeth Stefania"
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Item Susceptibilidad a los antimicóticos de levaduras aisladas de los sistemas de aguas de la ciudad de Santiago de Cali, Colombia(Universidad Santiago de Cali, 2019) Osorio Vanegas, Lizeth Stefania; Caicedo Bejarano, Luz Dary (Directora); Ramírez Castrillón, Mauricio (Director)Fungal infections increased markedly on the world. Currently, new pathogenic yeast species are reported with more frequence. The most important sources of aquatic isolation are industrial and municipal wastewaters, represented by chemical, physical and biological pollutants. The aim of this study was to characterize the antifungal susceptibility of yeasts isolated from, water systems in Cali, Colombia. Also to assens the correlation between physicochemical parameters, heavy metals, and fluconazole susceptibility, one of the most used antifungal. Two types of water were collected: potable (Río Meléndez, Puerto Mallarino) and residual wastewaters (Canal Sur, Planta de Tratamiento Cañaveralejo - PTAR). Serial dilutions were made and the samples were cultured on Dicloran Rose Bengal Chloramphenicol (DRBC) agar (Merck) and CHRomagarCandida (BBL) to count the CFU/mL. Study of susceptibility to Amphotericin and Fluconazole were made by the plate microdilution technique. Groupings were carried out according to their morphology and the identification of the representative isolates of each morphotype was carried out by biochemical kit (API 20C) and analysis of sequences of the ITS1-5.8S-IST2 region. 15 yeast species were identified with API 20C and six species by molecular biology corresponding to: Candida albicans, Pichia fermentans, Diutina catenulata, Rhodotorula mucilaginosa, Trichosporon coremiiforme and Pichia kluyveri. It was reported two morphotypes corresponding to new species of Candida.Item Water Quality, Heavy Metals, and Antifungal Susceptibility to Fluconazole of Yeasts from Water Systems(Multidisciplinary Digital Publishing Institute (MDPI), 2023-02) Caicedo Bejarano, Luz Dary; Osorio Vanegas, Lizeth Stefania; Ramírez Castrillón, Mauricio; Castillo, Jorge Enrique; Martínez Garay, Carlos Andrés; Chávez Vivas, MónicaAquatic environments could be reservoirs of pathogenic yeasts with acquired antifungal resistance. The susceptibility to antifungal agents of yeasts present in the wastewater and natural waters of the city of Cali was evaluated. Samples were taken from two types of water: drinking water (Meléndez River, drinking water treatment plant “Puerto Mallarino” in the Cauca River) and wastewater (South Channel of the Cauca River, “Cañaveralejo-PTAR” wastewater treatment plant). Physico-chemical parameters, heavy metal concentration, and yeast levels were determined using standard procedures. Yeasts were identified using API 20 C AUX (BioMérieux) and sequence analysis of the ITS1-5.8S-ITS2 and D1/D2 regions of the large subunit of the ribosome. Susceptibility assays against fluconazole and amphotericin B using the minimum inhibitory concentration (MIC) test were determined using the microdilution method. The influence of physico-chemical parameters and heavy metals was established using principal component analysis (PCA). Yeast counts were higher at WWTP “PTAR” and lower at Melendez River, as expected. A total of 14 genera and 21 yeast species was identified, and the genus Candida was present at all locations. Susceptibility tests showed a 32.7% resistance profile to fluconazole in the order DWTP “Puerto Mallarino = WWTP “PTAR” > South Channel “Navarro”.