Browsing by Author "Ortega, Juan David"
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Item Obtención de ácido peracético mediante un método alternativo basado en bio-blanqueo para lavado y desinfección de prendas hospitalarias(Universidad Santiago de Cali, 2025) Tobar Acosta, Valeria; Ortega, Juan David; Ríos Acevedo, Jhon Jairo (Director)The availability of Peracetic Acid (APA) in the presence of Hydrogen Peroxide (H₂O₂) was evaluated within an alternative bleaching and disinfection system (Triacetin (Tri)/ H₂O₂ catalyzed by Arylesterase (Ar)) for hospital garments (PUH) of the company Lavaclinicas S.A. For the quantification of APA, the permanganometric/iodometric technique was used. The research was carried out in two scenarios after confirming the positive availability of APA through the action of an enzyme that performs the perhydrolysis reaction. The first scenario consisted of determining the time interval of highest reaction yield, identified between 10 and 15 minutes. The second evaluated the reduction of PCA and DNA stains compared to the currently used system (TAED/ H₂O₂), achieving partial removal of PCA and total removal of DNA. In the first scenario, several experimental systems were designed in which variables such as quantities, pH adjustments, chaotropic agents, and ammonia and phosphate solutions were adjusted to hyperactivate the Ar enzyme. The results indicated that the phosphate solution achieved a maximum efficiency of approximately 60%. In the second scenario, stains were treated with and without the addition of detergent (WITH and WITHOUT, respectively). Although the detergent partially denatures the enzyme and affects the generation of APA, it facilitates bleaching and the removal of difficult-to-remove stains, such as PCA. The latter, produced by chlorhexidine gluconate and sodium hypochlorite, are visually similar to DNA but are significantly more difficult to remove than PUH. The alternative system demonstrated comparable efficiency to the current system. Therefore, it is recommended to add the detergent after the time interval of highest reaction efficiency to avoid interference with APA generation.