Browsing by Author "Moreno Murillo, Karen Yiseth"
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Item Implementación de un tren de filtración al vacío para mejorar la eficiencia y optimización del proceso de filtración por membrana en la identificación de Coliformes totales y Escherichia coli presentes en agua potable(Universidad Santiago de Cali, 2024) Moreno Murillo, Karen Yiseth; Realpe Lopera, Sandra Lorena (Directora); Aranaga Arias, Carlos Andrés (Director)This study addresses the optimization of the microbiological analysis of drinking water through the implementation of a vacuum train for membrane filtration. It was proposed that this technique improves the detection of total coliforms and Escherichia coli essential to evaluate water safety. The methodology included tests of selectivity, sterility and efficiency in Chromocult and PCA culture media, as well as the standardization of the filtration method with validation through control strains. The results obtained showed that there were no statistically significant differences between the conventional filtration system and the filtration train since in the results of the variance analysis a calculated F value of 0.04 was reached for the reproducibility test of the method used by two different analysts, as well as for the reproducibility test using the two filtration systems under the same conditions, a calculated F value of 0.45 was obtained, therefore it can be concluded that the implementation of the filtration train is effective and shows agreement with the results in Comparison with the conventional filtration system, and can be operated by two analysts Different under the same conditions.