Browsing by Author "Montehermoso Ramos, Kyara Stefania"
Now showing 1 - 1 of 1
Results Per Page
Sort Options
Item Determinación de la capacidad de formación de biopelículas en aislados de Escherichia coli resistentes a betalactámicos(Universidad Santiago de Cali, 2024) Montehermoso Ramos, Kyara Stefania; Aranaga Arias, Carlos Andrés (Director)Escherichia coli is a bacterium capable of forming biofilms on invasive medical devices such as catheters, urinary catheters, artificial respirators, etc. When the infection is caused by isolates of E. coli resistant to beta-lactam antibiotics, therapeutic options are severely limited, increasing patient morbidity and mortality. The main mechanism of resistance to beta-lactams is the production of beta-lactamase enzymes that hydrolyze and inactivate them. In Colombia, the blaKPC gene, encoding for KPC carbapenemase (Klebsiella pneumoniae Carbapenemase), is endemic in the country and, along with other enzymes like VIM and NDM, is primarily responsible for beta- lactam resistance. The objective of this study was to determine the biofilm formation capacity in isolates of beta-lactam-resistant E. coli. To achieve this, the presence of the blaKPC gene was detected using polymerase chain reaction, while the ability to form biofilms was assessed using the crystal violet assay. The results indicate that the blaKPC gene was present in 88% of the isolates evaluated, and 100% could form biofilms. These findings underscore the complexity of infections caused by beta-lactam-resistant E. coli and contribute to the development of more effective public health policies by hospital infection committees, tailored to the evolving bacterial resistance landscape, with the aim of reducing the prevalence of resistant strains at the hospital level.