Browsing by Author "Minayo Sanchez, Jhon Esneider"
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Item Identificación del potencial antimicrobiano del metilglioxal presente en la miel natural comercializada en la ciudad de Cali(Universidad Santiago de Cali, 2025-12-04) Minayo Sanchez, Jhon Esneider; Mercado Gonzalez, Sandra DanielaThis study evaluated the physicochemical properties, methylglyoxal (MGO) concentration, and antimicrobial activity of two multifloral honeys from the Macizo Colombiano, produced and marketed by the Apimacizo Association, an organization certified as a Green Business and holder of the País Sana sustainability seal. The samples included a liquid honey (ML) collected in the low-altitude area of La Vega, mainly derived from floral nectar, and a creamy honey (MC) obtained from the highlands of Almaguer and Santa Rosa, produced largely from the sap exudate of oak trees (Quercus humboldtii). Physicochemical parameters such as pH, density, color, and °Brix were determined, while methylglyoxal content was quantified using quantitative proton nuclear magnetic resonance (q¹H NMR). Antimicrobial activity was assessed through the agar well diffusion method against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. Both honeys exhibited an acidic pH (4.19–4.77) and a high soluble solids content (82–88 °Brix). MGO levels were low in both samples, with the liquid honey (4.67 mg/kg) containing significantly higher concentrations than the creamy honey (1.25 mg/kg). In antimicrobial assays, the liquid honey showed greater inhibitory capacity, particularly against S. aureus, while Gram-negative bacteria showed limited inhibition and C. albicans was not susceptible. In conclusion, the liquid honey from the Macizo Colombiano demonstrated superior physicochemical quality and antibacterial performance, suggesting a promising therapeutic potential against Gram-positive bacteria and reinforcing its added value within sustainable apiculture practices in the region.