Browsing by Author "Materon Luna, Daniela"
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Item Disminución de la resistencia a ampicilina en cepas de Staphylococcus aureus resistentes a meticilina a través de nanoliposomas funcionalizados con polímeros de Hidroxipropilmetilcelulosa y Carboximetilcelulosa(Universidad Santiago de Cali, 2023) Sáenz, María Valentina; Materon Luna, Daniela; Oñate Garzon, José Fernando (Director)The methicillin-resistant strain of Staphylococcus aureus (MRSA) causes infections that are resistant to most antibiotics, becoming a public health problem. The implementation of the delivery of ampicillin, a broad-spectrum antibiotic, was evaluated to avoid enzymatic degradation and achieve localized delivery that increases biological activity. For this, nanoliposomes coated with a non-ionic polymer such as hydroxypropylmethylcellulose (HPMC) and an anionic polymer such as carboxymethylcellulose (CMC) were used, which were prepared by the ethanol injection method and coated with the layer-by-layer method. Subsequently, the physicochemical characterization was carried out, using the Z-sizer equipment, to determine the size and find the surface charge (z potential). Then, by ultracentrifugation, the encapsulated ampicillin was separated and the encapsulation efficiency was extended. Then, the microbial susceptibility test was performed on two Staphylococcus aureus strains: sensitive ATCC25923 (MSSA) and resistant ATCC43300 (MRSA) to determine the minimum inhibitory concentration (MIC). Finally, variations in the size of the nanoliposomes functionalized with ampicillin-loaded polymers will be changed, which have various sizes (400-1000 nm), polydispersity from 0.4 to 0.7, zeta potential (-51 to -79 mV) and an encapsulation efficiency of about 92% in HPMC and 77% for CMC. However, the antibacterial effect was not as expected, since the nanosystems do not allow ampicillin to be fully released and can effectively reduce the bacterial resistance of the Staphylococcus aureus (MRSA) strain.