Browsing by Author "Martinez Arroyabe, Daniela"
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Item Caracterización microbiológica y molecular de transconjugantes obtenidos a partir de aislados de Escherichia coli resistentes a cefalosporinas provenientes de la ciudad de Cusco, Perú(Universidad Santiago de Cali, 2025) Martinez Arroyabe, Daniela; Correa Bermúdez, Adriana; Falco Restrepo , Aura DayanaEscherichia coli (E. coli) is a gram-negative, facultative anaerobic bacillus that lives in the intestines of warm-blooded animals and in the environment. In recent years, there has been a worldwide increase in resistance to beta-lactam antibiotics, especially third-generation cephalosporins. This increase has been reported to be associated with the horizontal transfer of the plasmid genes blaTEM, blaSHV, and blaCTX-M, through the process of conjugation, which represents an epidemiological risk to global public health. The frequency of third-generation cephalosporin-resistant E. coli isolates is high in Latin American countries, including Peru. In a previous study, transconjugants of cephalosporin-resistant E. coli isolates from Cusco, Peru, were obtained; however, the genes transferred by conjugation were not characterized. Therefore, in this study, the microbiological and molecular characterization of E. coli transconjugants obtained from third-generation cephalosporin-resistant isolates from Cusco was performed. This allowed us to detect the presence of the blaTEM gene in 7.1% and the blaCTX-M gene in 64.2% of the strains evaluated, with 36% of genes unidentified. In addition, the antibiotic sensitivity profiles of the transconjugants were determined, identifying that 85.71% of the transconjugants showed changes in the MIC with respect to E. coli J53. Based on the results obtained, the implementation of guidelines regarding the donor strain is recommended. These results show that the plasmid conjugation process of E. coli carrying BLEE is more efficient with CTX-M than with other genes, which is associated with the widespread dissemination of this enzyme worldwide