Browsing by Author "Jaramillo Ruiz, Felipe Antonio"
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Item Detección molecular de Leptospira spp en roedores plaga del Zoológico de Cali(Universidad Santiago de Cali, 2020) Jaramillo Ruiz, Felipe Antonio; Nieto Ramírez, Luisa María (Directora)Leptospirosis is a zoonosis caused by bacteria of the genus Leptospira spp, with rodents being its best known reservoir. The Zoo of Cali controls rodent plagues which potentially keep the circulation and presence of this bacterium active. To prove the presence of Leptospira spp and its pathogenic species among the plague rodents of the Cali Zoo, a total of 32 rats were captured between May and August 2019. Using samples of renal tissue, it was carried out the amplification by PCR of rrs and lipL32 genes, detecting a positivity of 12.5% for both genes. lipL32 also constitutes a molecular marker of pathogenicity whose product was also analyzed by Sanger sequencing, identifying L. interrogans species with a similarity of 100%. The results achieved allow confirming the presence of pathogenic species Leptospira spp in the renal tissue of rodents that circulate in the area of Cali Zoo, generating an evidence of the potential risk of transmission to other animal species, including visitors or workers of the institution.