Browsing by Author "Gaviria Cantin, Tania Cristina (Directora)"
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Item Evaluación del efecto antibacteriano de la actinidina del kiwi sobre el crecimiento de bacterias implicadas en la formación de caries(Universidad Santiago de Cali, 2020) Balanta Vidal, Milton Andrés; Gaviria Cantin, Tania Cristina (Directora)Dental caries is one of the most prevalent health problems of those affecting humans, however, for a long time it was not determined as a public health problem, as it does not generate direct mortality. Conventional treatment for the elimination of caries using low or high speed rotary instruments, often causing unfavorable reactions in patients, due to discomfort due to noise or the sensation of pressure on the oral cavity. For this reason, other treatment options have been sought that provide chemicalmechanical methods that offer more comfort. The goal of alternative fruit-based methods such as papaya containing proteolytic enzymes is to remove affected dentin by using manual instruments, to use remineralization and repair of the affected dentin. Taking this concept, in this study the antibacterial effect of actinidin enzyme extract from the kiwi fruit was evaluated. The growth of Streptococcus mutans and Lactobacillus casei bacteria involved in caries formation, was evaluated through in vitro assays. A previously used bacteria growth inhibition assay was performed using a kiwi extract (KiwiEnzyme ™ Actinidin Active P200) produced in New Zealand that provides standardized activity of the enzyme actinidin. The activity of actinidine on caries removal was also determined in an in vitro model by biofilm formation. A minimum inhibitory concentration (MIC) of 50 mg / ml was obtained for S. mutans and 170 mg / ml for L. casei, in both cases a bactericidal effect was affected because it managed to eliminate> 99.9% of the bacteria used in this study. The ability of actinidine to remove the biofilms formed by both bacteria was evident with significant differences between the control samples and the samples treated with the enzyme. In conclusion, the proven antimicrobial activity and removal of biofilms formed on the polystyrene surface by L. casei and S. mutans, allows us to propose the use of kiwi actinidin as a natural alternative for the treatment of caries.