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Browsing by Author "Gaviria Cantin, Tania"

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    First Report of Acanthocheilonema reconditum Outbreak in Canines with Clinical Signs of Anemia from Southwestern Colombia
    (2022-12) Espinosa, Nathaly; Rosero, Angelo; Villegas, Claudia Lucia; Garcia, Isabel Cristina; Gaviria Cantin, Tania; Nieto, Alejandra Peña; Ferro, Beatriz E.; Nieto Ramirez, Luisa Maria
    Different nematodes affect canines, however Acanthocheilonema reconditum was considered mostly a non-pathogenic parasite. Climate change, animal migration, and other factors transformed the dynamics of vector-borne diseases, including filariasis. Since 2016, a sudden increase in the number of dogs with microfilaremia was reported by different veterinary centers in Cali, southwest Colombia. The objective of this study was to molecularly identify the etiologic agent of this filariasis outbreak detected in this city, using PCR–RFLP and evaluating dogs’ clinical signs. From 2018–2019, canine filariasis cases were prospectively evaluated after a microscopic test, recruiting 82 cases and 43 healthy controls from 2971 samples. Acanthocheilonema reconditum (Nematoda, Onchocercidae) was identified in 61.3% of the cases (49/82) by PCR–RFLP. Sanger sequencing of the 5.8S ribosomal RNA gene and internal transcribed spacer-2 fragment was additionally performed on seven cases, confirming A. reconditum in all of them. The filariasis cases are statistically associated with male dogs who have clinical signs of anemia, low levels of hemoglobin and hematocrit (p < 0.0001), and high levels of plasma proteins (p < 0.001). This emerging canine disease constitutes an important public health concern among veterinarians and active surveillance is advised to explore its zoonotic potential.
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    Plasmodium, Trypanosoma y Salmonella: marcadores moleculares
    (Editorial Universidad Santiago de Cali, 2020-04-13) Vera Lizcano, Omaira; Grassini Gomes, Carla Sodré; Gustavo Zalis, Mariano; Tovar Acero, Nubia Catalina; Yasnot Acosta, Maria Fernanda; Castellanos Sánchez, Angélica; Loaeza Reyes, Karen Julissa; Moreno Rodríguez, Adriana; Torres Rosas, Rafael; Argueta Figueroa, Liliana; Zenteno Galindo, Edgar Arturo; Hernandez Cruz, Pedro Antonio; Gallegos Velasco, Itandehui Belem; Pérez Cervera, Tobana; Díez Mejía, Andrés Felipe; Marín Villa, Marcel; Varela Miranda, Rubén Eduardo; Gaviria Cantin, Tania; Balsalobre Parra, Carlos;
    Realizar este libro de revisión con temas de investigación actual, sin mostrar resultados, sino antecedentes del proceso investigativo, nos ha permitido contar de forma extensa los fundamentos teóricos de donde se genera la nueva investigación básica y la fundamentación para la investigación clínica. Este libro ha sido escrito por investigadores que han recopilado la información a través de sus carreras investigativas y que han visto cómo evoluciona el conocimiento gracias a la aplicación de la biología molecular como herramienta en el estudio de las enfermedades infecciosas. Cada capítulo del libro contiene información completa del conocimiento, hasta el día de hoy, de cada uno de los tópicos tratados; el tema en común son los biomarcadores moleculares de tres agentes infecciosos de importancia médica: Plasmodium spp., Trypanosoma spp. y Salmonella spp., agentes causales de enfermedades infecciosas de alta prevalencia en el mundo; las complicaciones que de ellas se derivan, como la sepsis y la falla multiorgánica, son importantes causas de morbilidad y mortalidad en los individuos afectados.

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