Browsing by Author "Falco Restrepo, Aura Dayana (Director)"
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Item Caracterización genética de aislados de Vibrio metschnikovii resistentes a antibióticos provenientes de una planta de tratamiento de lixiviados ubicada en Cali(Universidad Santiago de Cali, 2022) Zúñiga Espitia, Carolina; Falco Restrepo, Aura Dayana (Director)Vibrio metschnikovii is a Gram-negative bacillus that inhabits aquatic environments, including leachates, which are formed from the percolation of waste deposited in landfills. And that due to the high load of contaminants generate a selective pressure on bacteria, leading them to acquire extragenic elements such as plasmids that are disseminated from one bacterium to another through horizontal gene transfer, giving them characteristics that allow them to survive in adverse environments. Due to the high prevalence of V. metschnikovii isolates resistant to antibiotics in one of the lagoons without physicochemical treatment of a Leachate Treatment Plant, located in the municipality of Santiago de Cali, genetic characterization of these isolates was carried out in this work, with the objective of determining the genetic diversity of each one of the isolates. For this purpose, the isolates were genotyped by amplifying the extragenic palindromic repetitive sequences through the polymerase chain reaction (rep-PCR). Additionally, it was determined whether the isolates carried plasmids and whether they had the ability to transfer horizontally. The results indicate that, although rep sequences are found in a wide variety of bacteria, V. metschnikovii does not have them. For this reason, it is suggested that pulsed-field gel electrophoresis studies be carried out to determine the genetic relationship between bacterial isolates. Additionally, it was possible to purify plasmid DNA in an amikacin-resistant isolate, which cannot be transferred by conjugation.